ConductScience Cell Strainers 70um, Sterile (Bulk 10-Pack)
$52.00 per unit | 10 units per pack Bulk pricing: Bulk 10-Pack at $520.00 ($52.00 per unit)
| Automation Level | manual |
The ConductScience Cell Strainers 70um are sterile, single-use filtration devices designed for tissue digestion and cell isolation workflows. These nylon mesh strainers feature 70 micrometer pore size openings that effectively separate cellular suspensions from debris, undigested tissue fragments, and cell aggregates while allowing individual cells to pass through.
These strainers are essential tools for preparing single-cell suspensions from solid tissues in cell culture applications. The sterile packaging and construction maintain aseptic conditions during cell preparation procedures, making them suitable for primary cell isolation from various tissue types including neural, hepatic, and immune tissues.
How It Works
Cell strainers operate on the principle of size-exclusion filtration using a precisely woven nylon mesh. The 70 micrometer pore size allows individual cells (typically 10-30 micrometers in diameter) to pass through while retaining larger debris, cell clumps, and undigested tissue fragments. The nylon mesh provides consistent pore geometry and chemical resistance to common tissue digestion enzymes and buffers.
During use, tissue homogenates or enzymatically digested samples are applied to the strainer surface. Gentle agitation or pipetting forces the cell suspension through the mesh, while debris is retained on the upper surface. The filtered cell suspension collected below contains predominantly single cells suitable for downstream applications such as cell counting, viability assessment, or culture establishment.
Features & Benefits
Automation Level
- manual
Research Domain
- Cancer Research
- Cell Biology
- Developmental Biology
- Histopathology
- Immunology
- Neuroscience
Weight
- 30.92 kg
Dimensions
- L: 30.0 mm
- W: 25.0 mm
- H: 20.0 mm
Comparison Guide
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Pore Size Options | 70 micrometer nylon mesh | Standard options typically range from 40-100 micrometers | Optimal size for most mammalian cell separation while retaining tissue debris and aggregates. |
| Sterility Assurance | Individually sterile packaged | Some alternatives require user sterilization | Ready-to-use design saves preparation time and ensures consistent sterility. |
| Material Construction | Nylon mesh construction | Materials vary including stainless steel and polymer meshes | Chemical compatibility with tissue digestion enzymes and low protein binding properties. |
| Package Quantity | 50 units per package | Varies by model from individual to bulk packaging | Convenient quantity for routine cell culture workflows without excessive inventory. |
These cell strainers provide reliable single-cell suspension preparation with sterile packaging and chemical-resistant nylon construction. The 70um mesh size offers optimal separation for most mammalian cell types while maintaining high cell recovery rates.
Practical Tips
Pre-wet the mesh with sterile buffer before applying cell suspension to improve filtration efficiency and reduce cell adhesion.
Why: Hydrated mesh provides smoother flow and minimizes cell loss during processing.
Use gentle agitation or multiple small volume applications rather than forcing large volumes through at once.
Why: Prevents mesh clogging and reduces mechanical stress on cells during filtration.
Handle strainers using aseptic technique and dispose in biohazard waste containers after use.
Why: Maintains sterility and prevents contamination of subsequent cell culture work.
If flow rate decreases during filtration, rinse debris from mesh surface or use a fresh strainer for remaining sample.
Why: Accumulated debris can block pores and reduce filtration effectiveness.
Count cells before and after straining to assess recovery efficiency for your specific tissue type.
Why: Establishes baseline performance data for protocol optimization and quality control.
Store unused strainers in original sterile packaging at room temperature in a dry environment.
Why: Maintains sterility and prevents degradation of packaging integrity before use.
Setup Guide
What’s in the Box
- 50 individually packaged sterile cell strainers
- Product documentation and lot-specific information
Warranty
ConductScience provides standard quality assurance for cell culture consumables with replacement coverage for manufacturing defects. Technical support available for application-specific questions.
Compliance
What cell types can be processed with 70um mesh strainers?
70um mesh is suitable for most mammalian cell types including primary neurons, hepatocytes, immune cells, and cancer cells. Larger cells or cell aggregates will be retained while individual cells pass through.
Can these strainers be used with enzymatic digestion protocols?
Yes, the nylon mesh is compatible with common tissue digestion enzymes including collagenase, trypsin, and dispase without degradation or interference.
How should cell viability be assessed after straining?
Standard viability assays such as trypan blue exclusion or propidium iodide staining can be performed on filtered cell suspensions. Gentle filtration technique minimizes mechanical cell damage.
What is the maximum sample volume that can be processed?
Processing volume depends on tissue density and debris content. Multiple strainers may be required for large tissue samples to prevent mesh clogging.
Can the strainers be pre-wetted before use?
Pre-wetting with sterile buffer or culture medium can improve filtration efficiency and reduce cell adhesion to the mesh surface.
How do 70um strainers compare to other mesh sizes?
70um mesh provides intermediate filtration between 40um (finer, removes smaller debris) and 100um (coarser, faster flow but less debris removal) options.
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