ConductScience PCR Plate Sealing Film, Optical Clear (Case of 10)
$85.00 per unit | 10 units per pack Bulk pricing: Case of 10 at $850.00 ($85.00 per unit)
| Automation Level | manual |
Optical-grade adhesive sealing film designed for secure closure of PCR plates during thermal cycling and real-time PCR applications. The ultra-clear polyester construction provides excellent optical clarity for fluorescence-based detection systems while maintaining a strong adhesive seal throughout temperature cycling protocols.
Manufactured under controlled conditions to ensure DNase and RNase-free surfaces, preventing nucleic acid contamination that could compromise PCR amplification or quantification results. The optical transparency allows for direct fluorescence monitoring through the film during real-time PCR without requiring plate opening or seal removal.
How It Works
The sealing film consists of a transparent polyester base with a pressure-sensitive adhesive layer formulated for PCR applications. The adhesive creates a hermetic seal when applied to standard PCR plate surfaces, preventing sample evaporation during thermal cycling while maintaining optical clarity for fluorescence detection.
The ultra-clear polyester material exhibits minimal autofluorescence and maintains transparency across the visible and near-infrared spectrum used in real-time PCR systems. The adhesive formulation withstands repeated thermal cycling between ambient temperature and 95°C without delamination or seal failure, ensuring sample integrity throughout extended PCR protocols.
Features & Benefits
Automation Level
- manual
Research Domain
- Clinical Diagnostics
- Environmental Monitoring
- Food Science
- Microbiology
- Molecular Biology
- Pharmaceutical QC
Weight
- 30.92 kg
Dimensions
- L: 43.2 mm
- W: 36.0 mm
- H: 28.8 mm
Comparison Guide
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Optical Clarity | Ultra-clear polyester construction for excellent transparency | Standard plastic films may have reduced optical clarity | Enables direct fluorescence monitoring during real-time PCR without seal removal, improving workflow efficiency |
| Surface Treatment | DNase/RNase-free manufacturing process | Basic films may not guarantee nuclease-free surfaces | Prevents nucleic acid degradation that could compromise PCR results and data quality |
| Application Method | Pressure-sensitive adhesive for manual application | Heat-sealing films require specialized equipment | Simplifies sealing process and reduces equipment requirements for routine PCR protocols |
| Thermal Stability | Maintains seal integrity through PCR thermal cycling | Some adhesive films may fail at high temperatures | Ensures sample containment throughout complete thermal cycling protocols without seal failure |
This optical-grade sealing film combines ultra-clear transparency for real-time PCR monitoring with DNase/RNase-free manufacturing and reliable thermal cycling performance. The pressure-sensitive adhesive enables convenient manual application while maintaining hermetic seal integrity.
Practical Tips
Apply firm, even pressure across the entire film surface using a roller or applicator tool to ensure uniform adhesion.
Why: Uneven pressure can create air pockets or weak seal areas that may lead to sample evaporation during thermal cycling.
Avoid touching the adhesive surface during handling to prevent contamination and maintain sterility.
Why: Contact with skin oils or environmental contaminants can introduce PCR inhibitors or nucleases that compromise assay performance.
Store unused film in original packaging in a clean, dry environment at room temperature.
Why: Proper storage conditions maintain adhesive performance and prevent contamination of sterile surfaces.
If air bubbles form during application, work them out toward the edges before thermal cycling begins.
Why: Trapped air can expand during heating cycles and potentially compromise the seal integrity.
Verify optical clarity by checking for visible defects or scratches before sealing plates for real-time PCR.
Why: Surface imperfections can interfere with fluorescence detection and affect quantification accuracy.
Use appropriate personal protective equipment when handling the film to maintain aseptic conditions.
Why: Contamination prevention is critical for sensitive molecular biology applications and reliable PCR results.
Setup Guide
What’s in the Box
- 100 sheets of optical clear sealing film (typical)
- Product documentation with application guidelines (typical)
Warranty
ConductScience provides standard manufacturer warranty coverage for consumable products with technical support for application guidance and troubleshooting assistance.
Compliance
References
Background reading relevant to this product:
What temperature range can the sealing film withstand during PCR thermal cycling?
The film maintains seal integrity through thermal cycling protocols up to 95°C, which covers standard PCR denaturation temperatures, though specific temperature tolerance should be verified in the product datasheet.
Does the film interfere with fluorescence detection in real-time PCR systems?
The ultra-clear polyester construction and low autofluorescence properties minimize interference with fluorescence-based detection, allowing direct monitoring through the film without removal.
Is the film compatible with automated liquid handling systems?
The film is designed for manual application and may require removal before automated pipetting access, depending on the liquid handling system clearance specifications.
How should unused sealing film be stored to maintain performance?
Store in original packaging in a clean, dry environment at room temperature away from direct sunlight to prevent adhesive degradation and maintain sterility.
Can the film be reused or repositioned after initial application?
The pressure-sensitive adhesive is designed for single-use application, and repositioning may compromise seal integrity and introduce contamination risk.
What PCR plate formats are compatible with this sealing film?
The film is designed for standard 96-well and 384-well PCR plates from major manufacturers, though exact dimensional compatibility should be verified for specific plate models.
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