Information

Flat bottom restrainers are manufactured from perspex cylinders mounted on a flat black perspex base. All the models have free access through both extremes, by opening the respective tilting door that is fixed by a screw at the upper part of the restrainer.
Animals can be immobilized by sliding the doors along the restrainer. A slot in the base of the door allows for the whole tail to be available for the pulse transducer and cuff installation, the i.v drug administration, or the blood extraction.
The easy operation of the doors and their disposition on the extremes of the restrainer makes the manipulation of the animals convenient and avoids being dragged backward, protecting them from injuries in the feet.

Features

• Both head and tail can be adjusted.
• More exposure to animal areas in comparison to any other kind of restrainer.
• Screw and rod system provided for adjusting and nose piece is more in comparison to single knob system as in case of broom type restrainer.

Specifications

Protocol

• Grab the subject by the base of the tail with your dominant hand.
• Place the flat bottom restrainer on a smooth even surface, and orient it so that its open end makes an angle of 45° with the tabletop.
• Guide the subject into the restrainer, forelimbs first. Apply and maintain pressure to the subject’s rump so that it cannot retract back.
• Once the subject is in the tube, place the tailgate into the appropriate slot that fits the animal size to hold the subject into the flat bottom restrainer.

Benefits

• Front slit and pocket make it simple to feed and anesthetize the animal.
• Transparent material helps to analyze the animals visually.
• Our design eases the blood flow to almost every region of the animal’s body.
• The open bottom slot also facilitates the drainage of wastes.
• The front slits also provide sufficient ventilation.
• The animal is firmly gripped within the inner chamber (evoking minimum stress) so that efficient drug delivery to specific sites through specified routes is accomplished.

References

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C Mastronardi, G J Paz-Filho, E Valdez, J Maestre-Mesa, J Licinio, M-L Wong. (2011). Long-term body weight outcomes of antidepressant–environment interactions. Molecular Psychiatry, 16, 265–272.

Leonie A M Welberg, K V Thrivikraman, Paul M Plotsky. (2005). Chronic maternal stress inhibits the capacity to up-regulate placental 11-hydroxysteroid dehydrogenase type 2 activity. J Endocrinol,186(3):R7-R12.

Kristina M. Wright, Alyssa DiLeo, Michael A. McDannald. (2015). Early adversity disrupts the adult use of aversive prediction errors to reduce fear in uncertainty. Front Behav Neurosc, 27;9:227.

Alexander Oderhowho, Maria Victoria Tejada-Simon. (2015). Periconceptional stress in C57BL/6J female mice leads to altered behavioral responses in their offsprings. Annals of Neuroscience and Psychology.

Martin Williamson, Brenda Bingham, Megan Gray, Leyla Innala, Victor Viau. (2010). The Medial Preoptic Nucleus Integrates the Central Influences of Testosterone on the Paraventricular Nucleus of the Hypothalamus and Its Extended Circuitries. Journal of Neuroscience, 30 (35) 11762-11770.

Rachel K Rowe, Benjamin M Rumney, Hazel G May, Paska Permana, P David Adelson, S Mitchell Harman, Jonathan Lifshitz, Theresa C Thomas. (2016). Diffuse traumatic brain injury affects chronic corticosterone function in the rat. Endocr Connect, 5(4): 152–166.