Western Blot Electrophoresis Tank
Compact semi-dry electroblotting system for protein transfer from polyacrylamide gels to membranes, featuring 110×90mm transfer area and integrated temperature control.
Louise Corscadden, PhD
Director of Science · ConductScience
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Key Specifications
Full details →- Model fit
- Configured during quote
- SKU family
- BIO-0641
- Sizing
- 13.0 x 15.0 x 12.0 cm
- Ordering
- Online checkout and quote request available
- Category
- Gel & Protein Analysis
- Build notes
- Confirm accessories, station layout, and support needs before purchase
The Western Blot Electrophoresis Tank is a compact protein transfer system designed for semi-dry electroblotting applications in molecular biology and biochemistry laboratories. This system facilitates the transfer of separated proteins from polyacrylamide gels to membrane substrates for subsequent immunodetection analysis. The tank accommodates transfer gel areas up to 110×90mm with a 700ml buffer capacity, providing sufficient volume for consistent transfer conditions while maintaining a minimal laboratory footprint.
The system includes a complete transfer core assembly with positioning clips and cushioning components to ensure uniform protein transfer across the membrane surface. The integrated ice box component allows for temperature control during extended transfer protocols, preventing thermal degradation of heat-sensitive proteins and maintaining transfer efficiency.
How It Works
The electrophoretic transfer process utilizes an electric field to drive proteins from polyacrylamide gel matrices onto binding membranes through electrokinetic migration. Proteins maintain their relative positions from gel electrophoresis while being transferred perpendicular to the gel plane. The transfer buffer provides ionic conductivity and pH buffering to maintain protein stability during the migration process.
The semi-dry configuration positions the gel-membrane sandwich between parallel electrodes with buffer-saturated sponge cushions providing electrical contact. This design minimizes buffer volume requirements compared to wet transfer systems while maintaining uniform current distribution across the transfer area. The transfer core assembly ensures consistent pressure distribution and prevents air bubble formation that could disrupt protein migration patterns.
Features & Benefits
Automation Level
- manual
Transfer Gel Area(W*L)
- 110*90mm
Buffer Volume
- 700ml
Standard Accessories
- Body tank*1, Cap*1, Transfer core*1 set, Transfer clip*2, Sponge cushion*4, lce box*1
External Size(L*W*H)
- 150*120*130mm
Package Size(L*W*H)
- 290*290*200mm
Brand
- ConductScience
Research Domain
- Analytical Chemistry
- Cancer Research
- Cardiovascular
- Cell Biology
- Immunology
- Neuroscience
- Pharmaceutical QC
Weight
- 1.3kg
Weight
- 1.3 kg
Dimensions
- L: 13.0 mm
- W: 15.0 mm
- H: 12.0 mm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Transfer Area | 110×90mm | Entry-level models often offer smaller transfer areas | Accommodates standard mini-gel formats without requiring gel trimming or size adaptation. |
| Buffer Volume | 700ml capacity | Basic semi-dry units may require larger buffer volumes | Optimized volume reduces buffer waste while maintaining stable transfer conditions. |
| Temperature Control | Integrated ice box | Many basic models lack active cooling | Prevents thermal protein degradation during extended transfer protocols. |
| Complete Accessory Set | Includes tank, core, clips, cushions, ice box | Some systems require separate accessory purchases | Immediate operational capability without additional component requirements. |
This system combines the efficiency of semi-dry transfer with integrated temperature control and complete accessory inclusion. The optimized buffer capacity and transfer area dimensions provide reliable protein migration for standard laboratory gel formats.
Practical Tips
Pre-equilibrate all sponge cushions in transfer buffer for 10-15 minutes before assembly.
Why: Ensures uniform buffer saturation and prevents air bubble formation during transfer setup.
Clean the transfer core contacts with ethanol between uses and inspect for corrosion or deposits.
Why: Maintains electrical conductivity and prevents transfer artifacts from poor electrode contact.
If transfer efficiency appears reduced, verify gel-membrane contact pressure and eliminate air bubbles in the sandwich assembly.
Why: Air gaps prevent protein migration and create blank areas in the final blot.
Monitor buffer temperature throughout the transfer protocol and replenish ice as needed.
Why: Excessive heat can denature proteins and reduce transfer efficiency, particularly for large molecular weight targets.
Ensure all buffer levels are adequate before applying power and never operate with exposed electrodes.
Why: Prevents electrical hazards and maintains consistent current distribution across the transfer area.
Test transfer efficiency with molecular weight markers on each new membrane batch.
Why: Verifies consistent transfer performance and identifies any membrane lot-to-lot variations.
Setup Guide
What’s in the Box
- Body tank
- Tank cap
- Transfer core assembly
- Transfer clips (2 pieces)
- Sponge cushions (4 pieces)
- Ice box
- User manual (typical)
- Power cable (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering defects in materials and workmanship, with technical support for operational guidance and troubleshooting assistance.
Compliance
What membrane types are compatible with this transfer system?
The system accommodates standard protein transfer membranes including nitrocellulose, PVDF, and nylon membranes up to 110×90mm dimensions.
What voltage and current parameters should be used for optimal transfer?
Transfer parameters depend on protein molecular weight and membrane type; consult protocol literature for specific voltage, current, and timing recommendations for your target proteins.
How long does the ice box maintain cooling during transfer?
Cooling duration depends on ambient temperature and ice quantity; monitor buffer temperature throughout the transfer protocol and replenish ice as needed for extended procedures.
Can this system handle multiple gels simultaneously?
The single transfer core is designed for one gel per operation; multiple transfers require sequential processing or multiple units.
What cleaning protocols are recommended between uses?
Rinse all components with deionized water, clean with mild detergent if needed, and ensure complete drying before storage to prevent contamination.
Is this system compatible with chemiluminescent detection?
Yes, transferred proteins can be processed for any standard detection method including chemiluminescence, fluorescence, or colorimetric detection systems.
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