Endpoint methods library
Startle reflex endpoint

Acoustic startle amplitude

Peak or integrated movement response to a startling acoustic pulse, usually measured with a force plate, accelerometer, or startle chamber.

Unit
arbitrary units, grams-force, or volts
Readout
Peak or integrated startle response after an acoustic pulse
Assays
Acoustic startle, fear-potentiated startle, habituation, PPI baseline pulse trials

Decision summary

Use acoustic startle amplitude when the experiment needs a reflexive sensorimotor or hyperarousal readout. It can support questions about startle reactivity, habituation, threat sensitivity, or drug effects, but interpretation depends on hearing, body weight, chamber calibration, baseline movement, and trial history.

Primary valuePeak or integrated startle response after an acoustic pulse
Common unitsSystem-specific amplitude units, grams-force, volts, or normalized response
Compatible assaysAcoustic startle, fear-potentiated startle, habituation, PPI baseline pulse trials
Required boundaryPulse intensity, background noise, response window, and calibration method
Do not infer aloneFear, anxiety, hearing acuity, sensorimotor gating, or emotional state

Measurement notes

Define whether amplitude is peak response, average response, area under the response curve, or baseline-corrected movement. Include no-stimulus and pulse-alone trials so movement artifact and habituation can be separated from reflex amplitude.

Interpretation limit

Higher amplitude may reflect stronger reflex reactivity or hyperarousal, but it can also reflect body weight, restraint posture, chamber resonance, hearing threshold, baseline movement, sensitization, or trial-order effects.

Data capture

Store animal ID, trial type, pulse intensity, background noise, response window, baseline movement, peak amplitude, average amplitude, habituation block, chamber ID, calibration date, and exclusion flags.

Confound checks
  • Hearing impairment or unmeasured acoustic threshold differences.
  • Chamber calibration, sensor gain, body weight, or posture effects.
  • Baseline movement in the pre-pulse or pre-stimulus window.
  • Rapid habituation, sensitization, or trial-order imbalance.
  • Startle response saturation at very high pulse intensities.
Reporting checklist
  • Pulse intensity, duration, rise time, background noise, and response window.
  • Sensor type, calibration method, chamber model, and amplitude units.
  • Trial sequence, acclimation duration, inter-trial interval, and habituation blocks.
  • Baseline movement correction and exclusion rules for noisy trials.
  • Body weight, hearing screen or threshold control, strain, sex, and age.
  • Whether amplitude is peak, mean, integrated area, normalized, or block averaged.