Approach-Avoidance TTL Interface

4 events · BNC-6 multi-channel · 1 chamber

Overview

The Approach-Avoidance TTL interface provides 4 event signals for conflict paradigms where animals choose between appetitive and aversive zones. Events mark approach behavior, avoidance behavior, and trial boundaries — enabling correlation of approach-avoidance decisions with anxiety circuit recordings.

This interface is used with paradigms like elevated plus maze approach-avoidance conflicts, light-dark box, and reward-threat conflict tasks where the spatial choice between approach and avoidance is the primary dependent variable.

Setup Instructions

  1. Connect the TTL module via USB. Note COM port.
  2. In ConductMaze > TTL Settings, select BNC-6 for multi-channel output.
  3. Wire: Ch1 = trial boundaries, Ch2 = approach events, Ch3 = avoidance events.
  4. Calibrate zone boundaries in ConductMaze to match physical apparatus dimensions.
  5. Run a test trial and walk through each zone to verify event detection.

Common Pitfalls

  • Zone boundary definitions in software must precisely match physical sensor positions. Misalignment causes approach/avoidance events at the wrong spatial locations.
  • Animals often vacillate at zone boundaries, generating rapid approach/avoid event sequences. Consider applying a minimum dwell-time criterion in post-hoc analysis.

Methods Text (Copy-Paste)

Approach-avoidance behavior was synchronized with neural recordings using the ConductMaze TTL module (ConductScience, Skokie, IL). Four events (trial start/end, approach zone entry, avoidance zone entry) were transmitted via BNC-6 on separate channels using 9-byte frames at 115,200 baud with 10ms resolution.

Suggested text for the Methods section of your manuscript.

Compatible Products

ME-OC-TTL

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