ConductVision Organoid Monitor

Brain and neural organoids, monitored 24/7

In a pilot, ConductVision measures the growth, outline and folds of each visible brain or neural organoid, from images taken every 5 to 15 minutes.

Plan a pilotWhat a pilot records
Microscope image of an untreated human cerebral organoid on day 42: a dark, lobed organoid in the Matrigel it was embedded in.
Adapted from Zang et al. 2022 · CC BY 4.0

24/7 monitoring

What a pilot records for each brain or neural organoid

ConductVision records each measure for every visible organoid, keyed by plate, well, organoid ID and time.

MeasureWhat it showsHow often
Every organoid
Area
A brightfield image of spheroids, each outlined in yellow by OrganoSeg, a published tool, above three of them numbered beside the area OrganoSeg measured for each.
Adapted from Borten et al. 2018 · CC BY 4.0

The projected area of each organoid, with its longest and shortest diameters

Every 5 to 15 minutes
Growth rate
Phase-contrast images of an untreated cyst in a polycystic kidney disease organoid on days 1, 3, 7 and 14, growing from a small sphere to one that fills the frame.
Adapted from Gulieva et al. 2025 · CC BY 4.0

How fast each organoid grows or shrinks, per hour and per day

Every 5 to 15 minutes
Shape
Brightfield image of colorectal cancer organoids, round and irregular, each outlined in yellow by OrganoSeg, a published tool.
Adapted from Borten et al. 2018 · CC BY 4.0

How round, long or irregular each outline is

Every 5 to 15 minutes
Movement
Time-lapse microscope image of chicken embryo intestinal organoids in a gel, with dashed lines tracing how far six of them moved during the first day of culture.
Adapted from Pierzchalska et al. 2019 · CC BY 4.0

How far and how fast each organoid moves, where organoids are free to move

Every 5 to 15 minutes
Fusion
Time-lapse microscope images of chicken embryo intestinal organoids in a gel, in which two organoids, outlined in red and yellow, move together and fuse into one.
Adapted from Pierzchalska et al. 2019 · CC BY 4.0

Two organoids joining into one, with both parent IDs kept

Each time it happens
Fragmentation
Brightfield images of airway organoids 0, 24 and 48 hours after infection with a respiratory virus, breaking up over that time, beside mock-infected organoids at 48 hours.
Adapted from Stroulios et al. 2022 · CC BY 4.0

Organoids splitting or shedding pieces, each piece keeping its parent ID

Each time it happens
Structural collapse
Phase-contrast images of one polycystic kidney disease organoid cyst given 30 µM everolimus on days 0, 1, 3 and 14, shrinking from a round cyst to a small dark mass by day 1.
Adapted from Gulieva et al. 2025 · CC BY 4.0

A sudden loss of area, roundness or boundary, with a clip of the change

Each time it happens
Brain and neural organoids
Outline and folding
Brightfield microscope images of a brain organoid from a healthy control and one from a TUBB2A tubulinopathy line on day 30, each beside its outline drawn by hand, one lobed and one rounder.
Adapted from Schröter et al. 2024 · CC BY 4.0

How smooth, lobed or folded each organoid's outline is

Every 5 to 15 minutes
Neuroepithelial loops
Microscope images of human cerebral organoids on days 6, 10, 12 and 16, growing larger, with loops of neuroepithelium along their edges.
Adapted from Zang et al. 2022 · CC BY 4.0

Loops and folds of neuroepithelium along each organoid's edge, where the camera resolves them

Every 5 to 15 minutes
Protrusions
Microscope image of a control human cerebral organoid three days into differentiation, with neuroectodermal protrusions bulging from its surface.
Adapted from Gomez-Giro et al. 2019 · CC BY 4.0

Large protrusions as they form on each organoid's surface, where the camera resolves them

Every 5 to 15 minutes
Treatment response
Microscope images of human cerebral organoids on day 56, untreated and after 100, 250 or 500 µM valproic acid, the organoids smaller at the two higher doses.
Adapted from Zang et al. 2022 · CC BY 4.0

How growth, size and outline change after an exposure, against your controls

Every 5 to 15 minutes

When a fusion, fragmentation or structural collapse begins, the camera images that well every minute until the change ends. Fragmentation and structural collapse describe what the camera sees in each organoid's outline, not whether its cells are alive.

Beyond the camera

What needs higher magnification or a dye

  • Individual neurons and neurites
  • Nuclei
  • Neural rosettes
  • Cortical layers
  • Calcium signaling
Everything a camera cannot measure

Evidence

What published studies found in brain organoids

Other research groups measured size and growth changes like these in microscope images of brain organoids.

Brightfield microscope images of four brain organoids on day 30, one from each line: a healthy control and lines with TUBA1A tubulinopathy, TUBB2A tubulinopathy and tyrosine hydroxylase deficiency.
Adapted from Schröter et al. 2024 · CC BY 4.0

Four lines grew differently

Schröter and colleagues imaged 64 brain organoids from four iPSC lines on ten days between day 2 and day 30, on two microscopes.1 The lines grew in distinct patterns, and the TUBB2A line lagged behind the healthy line before catching up by day 30.1

Microscope images of human cortical organoids, untreated controls beside organoids exposed to ethanol, which are smaller.
Adapted from Adams et al. 2023 · CC BY 4.0

Smaller after ethanol

Adams and colleagues exposed cortical organoids about four weeks old to ethanol for seven days.2 At two months, the exposed organoids had a smaller diameter than controls, across 210 to 333 organoids per condition.2

Chart of cerebral organoid surface area on days 28, 42 and 56 after 0, 100, 250 or 500 µM valproic acid, lower at 250 and 500 µM by days 42 and 56.
Adapted from Zang et al. 2022 · CC BY 4.0

Smaller with valproic acid

Zang and colleagues added valproic acid to brain organoids while their neuroepithelium expanded.3 By days 42 and 56, organoids given 250 or 500 µM were smaller in surface area and diameter than controls, and 100 µM made no detectable difference.3

Cerebral organoids carrying a CLN3 mutation in a culture dish after 55 days of differentiation, with the small, underdeveloped ones circled in yellow by the authors.
Adapted from Gomez-Giro et al. 2019 · CC BY 4.0

Organoids that failed to develop

In cerebral organoids carrying a CLN3 mutation, about half completely failed to develop normally.4 On average, the mutant organoids were already slightly smaller than controls at the start of differentiation.4

Camera specification

The cameras a pilot uses

Microscope images of the same patient-derived intestinal organoids when forskolin was added and one hour later, when their lumens had filled with fluid.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Around the clock

  • A full stack of focal planes for every well, every 5 to 15 minutes
Brightfield image of mouse pancreas organoids, each with one large lumen, averaged from ten focal planes taken at 1.29 µm per pixel.
Adapted from Hof et al. 2021 · CC BY 4.0

Image

  • 2 µm or less per pixel
Human intestinal organoids in transmitted light, the dye-free imaging that OrgaSegment, a published tool, was built to analyze.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Light

  • Brightfield LED light, with no dyes or labels
Images of one human cortical organoid taken inside an incubator at 0, 43 and 86 hours, growing larger, beside a chart of its area at each time point.
Adapted from Ly et al. 2021 · CC BY 4.0

Incubator

  • Inside your incubator, or in a chamber beside it that keeps the same conditions
Full camera specification

References

  1. Schröter J, Deininger L, Lupse B, et al. (2024). A large and diverse brain organoid dataset of 1,400 cross-laboratory images of 64 trackable brain organoids. Scientific Data.
  2. Adams JW, Negraes PD, Truong J, et al. (2023). Impact of alcohol exposure on neural development and network formation in human cortical organoids. Molecular Psychiatry.
  3. Zang Z, Yin H, Du Z, et al. (2022). Valproic acid exposure decreases neurogenic potential of outer radial glia in human brain organoids. Frontiers in Molecular Neuroscience.
  4. Gomez-Giro G, Arias-Fuenzalida J, Jarazo J, et al. (2019). Synapse alterations precede neuronal damage and storage pathology in a human cerebral organoid model of CLN3-juvenile neuronal ceroid lipofuscinosis. Acta Neuropathologica Communications.
  5. Borten MA, Bajikar SS, Sasaki N, et al. (2018). Automated brightfield morphometry of 3D organoid populations by OrganoSeg. Scientific Reports.
  6. Gulieva RE, Ahmadvand P, Freedman BS (2025). A novel rapalog shows improved safety vs. efficacy in a human organoid model of polycystic kidney disease. Stem Cell Reports.
  7. Pierzchalska M, Panek M, Grabacka M (2019). The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids. Protoplasma.
  8. Stroulios G, Brown T, Moreni G, et al. (2022). Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs. Scientific Reports.
  9. Lefferts JW, Kroes S, Smith MB, et al. (2024). OrgaSegment: deep-learning based organoid segmentation to quantify CFTR dependent fluid secretion. Communications Biology.
  10. Hof L, Moreth T, Koch M, et al. (2021). Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis. BMC Biology.
  11. Ly VT, Baudin PV, Pansodtee P, et al. (2021). Picroscope: low-cost system for simultaneous longitudinal biological imaging. Communications Biology.

Plan a brain organoid pilot

Tell us about your brain or neural organoids, your plates and the first question you want answered.

Plan a pilotAll organoid models