ConductVision Organoid Monitor

Intestinal and colorectal organoids, monitored 24/7

In a pilot, ConductVision measures the growth, buds and lumens of every visible intestinal or colorectal organoid, from images taken every 5 to 15 minutes.

Plan a pilotWhat a pilot records
Low-magnification brightfield image of dozens of round, thin-walled human intestinal organoids of many sizes, several overlapping and some out of focus.
Adapted from Kassis et al. 2019 · CC BY 4.0

24/7 monitoring

What a pilot records for each intestinal or colorectal organoid

ConductVision records each measure for every visible organoid, keyed by plate, well, organoid ID and time.

MeasureWhat it showsHow often
Every organoid
Area
A brightfield image of spheroids, each outlined in yellow by OrganoSeg, a published tool, above three of them numbered beside the area OrganoSeg measured for each.
Adapted from Borten et al. 2018 · CC BY 4.0

The projected area of each organoid, with its longest and shortest diameters

Every 5 to 15 minutes
Growth rate
Phase-contrast images of an untreated cyst in a polycystic kidney disease organoid on days 1, 3, 7 and 14, growing from a small sphere to one that fills the frame.
Adapted from Gulieva et al. 2025 · CC BY 4.0

How fast each organoid grows or shrinks, per hour and per day

Every 5 to 15 minutes
Shape
Brightfield image of colorectal cancer organoids, round and irregular, each outlined in yellow by OrganoSeg, a published tool.
Adapted from Borten et al. 2018 · CC BY 4.0

How round, long or irregular each outline is

Every 5 to 15 minutes
Movement
Time-lapse microscope image of chicken embryo intestinal organoids in a gel, with dashed lines tracing how far six of them moved during the first day of culture.
Adapted from Pierzchalska et al. 2019 · CC BY 4.0

How far and how fast each organoid moves, where organoids are free to move

Every 5 to 15 minutes
Fusion
Time-lapse microscope images of chicken embryo intestinal organoids in a gel, in which two organoids, outlined in red and yellow, move together and fuse into one.
Adapted from Pierzchalska et al. 2019 · CC BY 4.0

Two organoids joining into one, with both parent IDs kept

Each time it happens
Fragmentation
Brightfield images of airway organoids 0, 24 and 48 hours after infection with a respiratory virus, breaking up over that time, beside mock-infected organoids at 48 hours.
Adapted from Stroulios et al. 2022 · CC BY 4.0

Organoids splitting or shedding pieces, each piece keeping its parent ID

Each time it happens
Structural collapse
Phase-contrast images of one polycystic kidney disease organoid cyst given 30 µM everolimus on days 0, 1, 3 and 14, shrinking from a round cyst to a small dark mass by day 1.
Adapted from Gulieva et al. 2025 · CC BY 4.0

A sudden loss of area, roundness or boundary, with a clip of the change

Each time it happens
Intestinal and colorectal organoids
Buds
Differential interference contrast image of one mouse intestinal organoid with many buds extending from its body.
Adapted from Goveas et al. 2021 · CC BY 4.0

How many gross buds each organoid has, and how fast new ones form

Every 5 to 15 minutes
Lumens
Microscope images of patient-derived intestinal organoids with a rare CFTR genotype when CFTR modulators were added and 24 hours later, when their lumens had filled with fluid.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Visible lumens as they form and swell

Every 5 to 15 minutes
Cystic or budding
Microscope images of mouse colon organoids on day 6 of culture, two round, cystic colonospheres above and two colonoids with buds extending into the gel below.
Adapted from Yip et al. 2018 · CC BY 4.0

Whether each organoid is a round cyst or a budding organoid

Every 5 to 15 minutes
Treatment response
Microscope images of chicken embryo intestinal organoids grown with no additions, with LPS, with Y-27632 or with both, where LPS gave larger organoids and Y-27632 more, smaller ones.
Adapted from Pierzchalska et al. 2019 · CC BY 4.0

How growth, buds and lumens change after a treatment, against your controls

Every 5 to 15 minutes

When a fusion, fragmentation or structural collapse begins, the camera images that well every minute until the change ends. Fragmentation and structural collapse describe what the camera sees in each organoid's outline, not whether its cells are alive.

Beyond the camera

What needs higher magnification or a dye

  • Individual cells
  • Cell polarity
  • Fine epithelial layers
  • Cell layout inside crypts
Everything a camera cannot measure

Evidence

What published studies found in intestinal organoids

Other research groups measured these in microscope images of intestinal organoids.

Microscope images of patient-derived intestinal organoids with a rare CFTR genotype when CFTR modulators were added and 25 hours later, an hour after forskolin, when their lumens had swollen.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Swelling

Forskolin made intestinal organoids from healthy controls swell rapidly, and organoids from people with cystic fibrosis swelled far less.1 The swelling matched forskolin-induced anion currents in rectal biopsies, which made it a functional CFTR assay.1

Brightfield images of intestinal organoids 0, 20, 40 and 60 minutes into forskolin-induced swelling, each organoid covered by a colored mask that OrgaSegment, a published tool, drew.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Swelling in brightfield

Earlier swelling assays relied on fluorescent labels, which leak out over time and limit how long organoids can be followed.2 OrgaSegment outlined each patient-derived intestinal organoid in brightfield images, measured its swelling and told apart organoids with different CFTR mutations.2

Differential interference contrast images of mouse intestinal organoids, control organoids with buds above and mutant organoids that lost their buds and turned cyst-like below.
Adapted from Goveas et al. 2021 · CC BY 4.0

Cystic or budding

SWIFT, a published brightfield pipeline, sorted intestinal organoids into canonical forms, among them cystic, columnar and budding, by area, circularity and brightness.3

Microscope images of colorectal cancer organoids at 50 and 52 hours of culture, in which a swollen, hollow organoid collapsed into a small dark mass, marked by the authors' arrow.
Adapted from Yan et al. 2024 · CC BY 4.0

Growth and collapse

Following single organoids over time, the SWIFT authors found that growth rates correlated with Wnt concentration and that rapidly expanding organoids tended to collapse earlier.3

Camera specification

The cameras a pilot uses

Microscope images of the same patient-derived intestinal organoids when forskolin was added and one hour later, when their lumens had filled with fluid.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Around the clock

  • A full stack of focal planes for every well, every 5 to 15 minutes
Brightfield image of mouse pancreas organoids, each with one large lumen, averaged from ten focal planes taken at 1.29 µm per pixel.
Adapted from Hof et al. 2021 · CC BY 4.0

Image

  • 2 µm or less per pixel
Human intestinal organoids in transmitted light, the dye-free imaging that OrgaSegment, a published tool, was built to analyze.
Adapted from Lefferts et al. 2024 · CC BY 4.0

Light

  • Brightfield LED light, with no dyes or labels
Images of one human cortical organoid taken inside an incubator at 0, 43 and 86 hours, growing larger, beside a chart of its area at each time point.
Adapted from Ly et al. 2021 · CC BY 4.0

Incubator

  • Inside your incubator, or in a chamber beside it that keeps the same conditions
Full camera specification

References

  1. Dekkers JF, Wiegerinck CL, de Jonge HR, et al. (2013). A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nature Medicine.
  2. Lefferts JW, Kroes S, Smith MB, et al. (2024). OrgaSegment: deep-learning based organoid segmentation to quantify CFTR dependent fluid secretion. Communications Biology.
  3. Bracq L, Guiet R, Offner S, et al. (2026). A Single-organoid Workflow for quantitative Imaging classiFication and Tracking (SWIFT). Communications Biology.
  4. Kassis T, Hernandez-Gordillo V, Langer R, et al. (2019). OrgaQuant: Human Intestinal Organoid Localization and Quantification Using Deep Convolutional Neural Networks. Scientific Reports.
  5. Borten MA, Bajikar SS, Sasaki N, et al. (2018). Automated brightfield morphometry of 3D organoid populations by OrganoSeg. Scientific Reports.
  6. Gulieva RE, Ahmadvand P, Freedman BS (2025). A novel rapalog shows improved safety vs. efficacy in a human organoid model of polycystic kidney disease. Stem Cell Reports.
  7. Pierzchalska M, Panek M, Grabacka M (2019). The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids. Protoplasma.
  8. Stroulios G, Brown T, Moreni G, et al. (2022). Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs. Scientific Reports.
  9. Goveas N, Waskow C, Arndt K, et al. (2021). MLL1 is required for maintenance of intestinal stem cells. PLOS Genetics.
  10. Yip HYK, Tan CW, Hirokawa Y, et al. (2018). Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts. PLOS ONE.
  11. Yan H, Wu T, Li X, et al. (2024). Establishment of the microscope incubation system and its application in evaluating tumor treatment effects through real-time live cellular imaging. Frontiers in Bioengineering and Biotechnology.
  12. Hof L, Moreth T, Koch M, et al. (2021). Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis. BMC Biology.
  13. Ly VT, Baudin PV, Pansodtee P, et al. (2021). Picroscope: low-cost system for simultaneous longitudinal biological imaging. Communications Biology.

Plan an intestinal organoid pilot

Tell us about your intestinal or colorectal organoids, your plates and the first question you want answered.

Plan a pilotAll organoid models