Research data ยท Depression-like behavior

Splash test

From 2021 to 2025, 132 animal research papers mentioned the splash test in their title or abstract, ranking it 51st of the 90 tests we track.

That is 6.9 times the 19 papers of 2011 to 2015. Papers that mention any of the 90 tests grew to 1.2 times as many over the same span.

132 animal research papers from 2021 to 2025 mentioned the test
6.9 times as many papers as in 2011 to 2015
51st of the 90 tests by papers from 2021 to 2025
67% of these papers used mice and 32% used rats

Papers by year

Animal research papers that mention the splash test, by year, 2001 to 2026

The 2026 bar counts papers added to PubMed by November 8, 2026.

  • Papers in a full year
  • 2026 so far
Show the counts for each year
Papers by year
YearPapers
20010
20020
20030
20040
20051
20060
20071
20084
20091
20101
20111
20125
20135
20144
20154
201613
201716
201818
201915
202026
202117
202227
202322
202439
202527
2026 (part year)36

Papers that mention the splash test in mice and in rats, by year, 2001 to 2025

  • Mouse
  • Rat
Show the counts for each year and species
Papers by year, mice and rats
YearMouseRat
200100
200200
200300
200400
200510
200600
200710
200840
200910
201010
201110
201250
201350
201440
201540
2016112
2017134
2018117
2019132
2020215
2021124
2022215
2023166
20242515
20251512

Which species the papers used

Each bar is a share of the 132 papers from 2021 to 2025. A paper can use more than one species, so the shares can add to more than 100%.

Species

Mouse
89 papers 67%
Rat
42 papers 32%
Other animals
2 papers 2%

โ€œOther animalsโ€ counts papers that use none of the other species in this chart.

Strains and genetic lines

Strain names come from the MeSH terms PubMed adds when it indexes a paper. PubMed has indexed 106 of the 132 papers, so each bar is a share of those 106.

C57BL mice
24 papers 23%
Wistar rats
19 papers 18%
Sprague-Dawley rats
11 papers 10%
Knockout mice
2 papers 2%
Transgenic mice
2 papers 2%
ICR mice
2 papers 2%
Spontaneously hypertensive rats
1 paper 1%
Other inbred mice
1 paper 1%

A paper indexed under two of these headings, such as knockout mice and a strain, counts under both.

Disease models these papers study

Each bar counts the papers from 2021 to 2025 that mention the splash test and study that disease model, with its share of the 132 papers. The model comes from a paper's MeSH terms. For a paper PubMed has not indexed, it comes from PubTator 3, NCBI's text-mining service, when that service finds the disease in the title or abstract. A paper can count under more than one model.

Papers from 2021 to 2025 by disease model

Tests mentioned alongside the splash test

Each bar counts the papers from 2021 to 2025 that mention both tests, with its share of the 132 papers that mention the splash test.

Papers from 2021 to 2025 that mention both tests

63 papers 48%
46 papers 35%
42 papers 32%
10 papers 8%

The most cited papers from 2021 to 2025

These papers from 2021 to 2025 mention the splash test and have the most citations, as counted by NIH's iCite on November 5, 2026. We show four of the five most cited papers and leave out one because of words in its title.

  1. Limonene through Attenuation of Neuroinflammation and Nitrite Level Exerts Antidepressant-Like Effect on Mouse Model of Maternal Separation Stress.

    Behav Neurol, 2021. 53 citations.

  2. Sex Differences in Depression-Like Behaviors in Adult Mice Depend on Endophenotype and Strain.

    Front Behav Neurosci, 2022. 38 citations.

  3. Effects of corticosterone on BDNF expression and mood behaviours in mice.

    Physiol Behav, 2022. 34 citations.

  4. Neuroprotective Effect of Apigenin on Depressive-Like Behavior: Mechanistic Approach.

    Neurochem Res, 2022. 34 citations.

How we counted

A count is the number of animal research papers whose title or abstract mentions one of the test's search terms. It is not a count of experiments, labs or animals.

A paper that mentions the splash test only in its methods is missed, so the count runs low. The count includes 0 reviews. For 26 of the 132 papers, PubMed's indexing gave no species, so the species came from PubTator 3 finding one in the title or abstract.

The test's search terms are in the test list download. The full method, with each source and its terms of use, is on our main page, Behavioral tests in published research.

More depression-like behavior tests

These depression-like behavior tests also have a page, with their papers from 2021 to 2025. Each test with 30 or more papers gets one.

How to cite

APA

ConductScience. (2026, November 10). Splash test in published research [Data set]. https://conductscience.com/data/behavioral-tests/splash-test

BibTeX

@misc{conductscience_splash_test_2026,
  author = {{ConductScience}},
  title = {Splash test in published research},
  year = {2026},
  month = nov,
  url = {https://conductscience.com/data/behavioral-tests/splash-test},
  note = {Data set, released November 10, 2026},
}