Research data · Released November 10, 2026

Behavioral tests in published research

We counted how many animal research papers mention each of 90 behavioral tests in their title or abstract, and which species those papers used.

Both counts run by year from 2001. We searched 39.7 million PubMed records, up to November 8, 2026.

32,277 animal research papers from 2021 to 2025 mentioned at least one test
36% of those papers mentioned two or more tests
2020 the year mice overtook rats in papers that mention a test, after rats led every year from 2001
90 behavioral tests counted

From 2021 to 2025, mice appear in 51% of the papers that mention a test and rats in 42%. Some papers use both.

The 90 tests, ranked by papers from 2021 to 2025

Each row gives a test's papers from 2021 to 2025 and the change compared with 2011 to 2015. Pick a behavioral domain, such as pain and nociception or anxiety-like behavior, to narrow the list.

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All 90 tests.

The behavioral tests, with their papers in 2021 to 2025
Morris water maze Spatial learning and memory5,3921.6 timesMice 51%, rats 51%Shop the apparatus
Open field Activity and exploration3,9802.1 timesMice 55%, rats 42%Shop the apparatus
Novel object recognition Recognition memory3,0192.3 timesMice 57%, rats 40%Shop the apparatus
Forced swim test Depression-like behavior2,9881.2 timesMice 58%, rats 44%Shop the apparatus
Elevated plus maze Anxiety-like behavior2,8831.3 timesRats 51%, mice 50%Shop the apparatus
Treadmill running Motor function and endurance2,0911.2 timesMice 48%, rats 42%Shop the apparatus
Fear conditioning Fear and avoidance2,010about the sameMice 58%, rats 35%Shop the apparatus
Y-maze Working memory1,9882.9 timesMice 64%, rats 32%Shop the apparatus
Rotarod Motor function and endurance1,8441.4 timesMice 67%, rats 36%Shop the apparatus
Sucrose preference test Depression-like behavior1,5222.8 timesMice 52%, rats 49%Shop the apparatus
Tail suspension test Depression-like behavior1,4051.9 timesMice 89%, rats 14%Shop the apparatus
Self-administration Reward and motivation1,3999% fewerRats 70%, mice 25%Shop the apparatus
Grip strength Motor function and endurance1,3542.9 timesMice 71%, rats 25%Shop the apparatus
Von Frey test Pain and nociception1,2821.6 timesRats 53%, mice 49%Shop the apparatus
Passive avoidance Fear and avoidance1,14210% fewerRats 56%, mice 42%Shop the apparatus
Conditioned place preference Reward and motivation1,07811% fewerMice 60%, rats 39%Shop the apparatus
Wheel running Activity and exploration9921.1 timesMice 74%, rats 25%Shop the apparatus
Operant conditioning Reward and motivation94524% fewerRats 55%, mice 32%Shop the apparatus
Gait analysis Motor function and endurance8212.1 timesMice 37%, rats 35%Shop the apparatus
Prepulse inhibition and acoustic startle Sensory function and startle80423% fewerMice 43%, rats 37%
Hot plate Pain and nociception7628% fewerMice 62%, rats 44%Shop the apparatus
Light-dark box Anxiety-like behavior7251.5 timesMice 48%, rats 30%Shop the apparatus
Formalin test Pain and nociception54531% fewerMice 65%, rats 42%
Tail flick Pain and nociception54533% fewerMice 52%, rats 50%Shop the apparatus
Writhing test Pain and nociception52833% fewerMice 79%, rats 37%
Barnes maze Spatial learning and memory4992.6 timesMice 64%, rats 36%Shop the apparatus
Beam walking Motor function and endurance4911.7 timesMice 52%, rats 48%Shop the apparatus
Social interaction test Social behavior4821.5 timesMice 44%, rats 41%Shop the apparatus
Self-grooming Home-cage behavior4471.6 timesMice 47%, rats 27%
T-maze Working memory4361.1 timesMice 41%, rats 38%Shop the apparatus
Nest building Home-cage behavior4222.0 timesMice 48%, other animals 45%
Object location test Spatial learning and memory3391.9 timesMice 60%, rats 40%
Three-chamber sociability test Social behavior3394.8 timesMice 63%, rats 35%Shop the apparatus
Marble burying Anxiety-like behavior3281.9 timesMice 77%, rats 26%
Negative geotaxis and climbing assays Motor function and endurance3052.2 timesFruit flies 66%, rats 20%
Novelty-suppressed feeding Depression-like behavior2601.6 timesMice 63%, rats 40%
Novel tank test Anxiety-like behavior2455.3 timesZebrafish 96%
Catalepsy bar test Motor function and endurance24427% fewerRats 54%, mice 51%Shop the apparatus
Radial arm maze Spatial learning and memory22538% fewerRats 55%, mice 40%Shop the apparatus
Active avoidance (shuttle box) Fear and avoidance21017% fewerRats 53%, mice 33%Shop the apparatus
Hole-board Activity and exploration20613% fewerMice 62%, rats 37%
Wire hang and inverted grid Motor function and endurance2062.4 timesMice 73%, rats 29%
Predator odor exposure Fear and avoidance1858% fewerRats 44%, mice 37%Shop the apparatus
Hargreaves plantar test Pain and nociception174about the sameMice 53%, rats 49%Shop the apparatus
Cylinder test Motor function and endurance1631.5 timesRats 47%, mice 44%Shop the apparatus
Pole test Motor function and endurance1612.2 timesMice 85%, rats 18%Shop the apparatus
Grid walking (foot fault) Motor function and endurance1551.4 timesMice 55%, rats 48%Shop the apparatus
Touchscreen operant tasks Attention and executive function1463.2 timesMice 60%, rats 25%Shop the apparatus
Elevated zero maze Anxiety-like behavior1361.3 timesMice 63%, rats 39%Shop the apparatus
Resident-intruder test Social behavior1346% fewerMice 55%, rats 34%Shop the apparatus
Splash test Depression-like behavior1326.9 timesMice 67%, rats 32%
Attentional set shifting Attention and executive function11323% fewerRats 68%, mice 27%Shop the apparatus
Rotational behavior (rotometer) Motor function and endurance11048% fewerRats 46%, mice 44%Shop the apparatus
Adhesive removal test Motor function and endurance1071.4 timesMice 52%, rats 50%
Ladder rung walking Motor function and endurance1041.4 timesRats 58%, mice 41%Shop the apparatus
Five-choice serial reaction time task Attention and executive function10341% fewerRats 58%, mice 38%Shop the apparatus
Step-down avoidance Fear and avoidance9751% fewerMice 68%, rats 30%Shop the apparatus
Skilled reaching Motor function and endurance9533% fewerRats 55%, mice 45%Shop the apparatus
Looming stimulus Fear and avoidance922.0 timesMice 49%, other animals 35%Shop the apparatus
Acetone test Pain and nociception851.6 timesRats 65%, mice 36%
Learned helplessness Depression-like behavior8533% fewerRats 53%, mice 47%Shop the apparatus
Olfactory tests Sensory function and startle811.4 timesMice 62%, rats 28%
Cold plate Pain and nociception741.9 timesMice 58%, rats 43%Shop the apparatus
Paw pressure test Pain and nociception7045% fewerRats 77%, mice 29%
Drug discrimination Reward and motivation6546% fewerRats 74%, mice 40%Shop the apparatus
Corner test Motor function and endurance591.8 timesMice 58%, rats 49%
Social approach and avoidance Social behavior542.5 timesMice 74%, rats 17%Shop the apparatus
Tube test for social dominance Social behavior513.9 timesMice 71%, rats 29%Shop the apparatus
Radial arm water maze Spatial learning and memory4851% fewerRats 56%, mice 44%Shop the apparatus
Burrowing Home-cage behavior401.7 timesMice 65%, rats 23%
Puzzle box Attention and executive function34Too few papers to compareOther animals 50%, mice 44%Shop the apparatus
Thermal gradient and temperature preference Pain and nociception31Too few papers to compareMice 58%, other animals 26%Shop the apparatus
Elevated T-maze Anxiety-like behavior2960% fewerRats 83%, mice 17%
Observational fear Fear and avoidance26Too few papers to compareMice 92%Shop the apparatus
Active place avoidance Spatial learning and memory2411% fewerMice 75%, rats 38%Shop the apparatus
Social operant tasks Social behavior23Too few papers to compareRats 52%, mice 30%Shop the apparatus
Real-time place preference Reward and motivation19Too few papers to compareMice 89%, rats 21%Shop the apparatus
Plus maze for place and response learning Spatial learning and memory1641% fewerMice 63%, rats 38%Shop the apparatus
Runway Reward and motivation1248% fewerMice 42%, other animals 42%Shop the apparatus
Three-choice serial reaction time task Attention and executive function9Too few papers to compareMice 56%, rats 33%Shop the apparatus
Cincinnati water maze Spatial learning and memory8Too few papers to compareRats 100%Shop the apparatus
Hebb-Williams maze Spatial learning and memory8Too few papers to compareMice 50%, rats 50%
Vogel conflict test Anxiety-like behavior8Too few papers to compareRats 88%, other animals 13%Shop the apparatus
Water Y-maze Working memory4Too few papers to compareMice 75%, rats 25%Shop the apparatus
3D open field Activity and exploration1Too few papers to compareMice 100%Shop the apparatus
3D radial maze Spatial learning and memory1Too few papers to compareMice 100%Shop the apparatus
Bow-tie maze Recognition memory0Too few papers to compareNo papers
Concatenated Y-maze Working memory0Too few papers to compareNo papersShop the apparatus
Continuous object recognition Recognition memory0Too few papers to compareNo papersShop the apparatus
Dry-land Morris maze Spatial learning and memory0Too few papers to compareNo papersShop the apparatus

A change shows only when a test has 10 or more papers in both periods. A change under 5% shows as “about the same”. All tests together grew to 1.2 times as many. Treadmill running and wheel running include papers that used them as exercise. A paper can use more than one species, so a test's species shares can add to more than 100%.

Tests by behavioral domain

Each domain shows how many papers from 2021 to 2025 mentioned any of its tests. A paper that mentions tests from two domains counts under both.

Recognition memory

3,019 papers

2 more tests under Recognition memory in the full list

Working memory

2,404 papers

1,988 papers
436 papers
Water Y-maze
4 papers

1 more test under Working memory in the full list

Tests mentioned together

Among the 36% of papers from 2021 to 2025 that mentioned two or more tests, these pairs came up most often.

Papers from 2021 to 2025 that mention both tests

Open field and elevated plus maze
939 papers
Open field and forced swim test
896 papers
Forced swim test and tail suspension test
893 papers
Forced swim test and sucrose preference test
863 papers
Morris water maze and novel object recognition
846 papers
Forced swim test and elevated plus maze
646 papers
Novel object recognition and Y-maze
609 papers
Morris water maze and Y-maze
607 papers

Tests by disease model

Each row counts the papers from 2021 to 2025 that mention a test and study that disease model. The model comes from a paper's MeSH terms. For a paper PubMed has not indexed, it comes from PubTator 3 when that service finds the disease in the title or abstract. A paper can count under more than one model.

Papers from 2021 to 2025 by disease model, with the test they mention most

Cognitive impairment and dementiaMost often the Morris water maze, in 54% of the papers
4,961 papers
AnxietyMost often the elevated plus maze, in 51% of the papers
3,041 papers
DepressionMost often the forced swim test, in 64% of the papers
2,827 papers
Pain conditionsMost often the von Frey test, in 38% of the papers
2,617 papers
Alzheimer diseaseMost often the Morris water maze, in 60% of the papers
2,445 papers
Substance use disordersMost often self-administration, in 50% of the papers
1,599 papers
Psychological stressMost often the forced swim test, in 42% of the papers
1,484 papers
Stroke and brain ischemiaMost often the Morris water maze, in 32% of the papers
1,278 papers
Parkinson diseaseMost often the rotarod, in 36% of the papers
918 papers
Brain injuryMost often the Morris water maze, in 38% of the papers
841 papers
DiabetesMost often the Morris water maze, in 34% of the papers
795 papers
Obesity and high-fat dietMost often treadmill running, in 21% of the papers
761 papers
AgingMost often the Morris water maze, in 26% of the papers
700 papers
Epilepsy and seizuresMost often the Morris water maze, in 23% of the papers
572 papers
Autism spectrum disorderMost often the three-chamber sociability test, in 27% of the papers
484 papers
Schizophrenia and psychosisMost often prepulse inhibition and acoustic startle, in 40% of the papers
401 papers
Sleep loss and sleep disordersMost often the Morris water maze, in 28% of the papers
400 papers
Post-traumatic stressMost often fear conditioning, in 57% of the papers
392 papers
Spinal cord injuryMost often gait analysis, in 33% of the papers
382 papers
Multiple sclerosis and demyelinationMost often the rotarod, in 30% of the papers
223 papers
Amyotrophic lateral sclerosisMost often the rotarod, in 50% of the papers
108 papers
Attention deficit hyperactivity disorderMost often the open field, in 32% of the papers
107 papers
Huntington diseaseMost often the rotarod, in 51 of the 96 papers
96 papers
Fragile X syndromeMost often the open field, in 8 of the 38 papers
38 papers
Down syndromeMost often fear conditioning, in 3 of the 16 papers
16 papers

Huntington disease, fragile X syndrome and Down syndrome have fewer than 100 papers each, so they have no page of their own. Their papers are in the downloads.

How test use changed

Animal research papers that mention a test grew from 2,404 in 2001 to 7,115 in 2025, 3.0 times as many. Counting only papers the National Library of Medicine indexed, those that mention a test grew to 2.5 times as many over the same years, and all animal research papers to 1.7 times as many. The table of all 90 tests compares two five-year periods: 26,411 papers mentioned a test in 2011 to 2015 and 32,277 in 2021 to 2025, 1.2 times as many.

  • Among tests of depression-like behavior, the anhedonia and grooming tests grew fastest. Sucrose preference papers grew from 542 to 1,522, 2.8 times as many, and splash test papers from 19 to 132, 6.9 times as many. Forced swim papers grew to 1.2 times as many, the same as all tests together.
  • Among pain tests, von Frey papers grew from 780 to 1,282, 1.6 times as many, while tail flick, writhing and formalin test papers each fell by about a third.
  • The novel tank test, run in zebrafish in 96% of its papers, grew from 46 papers to 245, 5.3 times as many, while all tests together grew to 1.2 times as many.
  • Of the three social tests with 100 or more papers in 2021 to 2025, the three-chamber sociability test grew fastest, from 71 papers to 339, 4.8 times as many. Social interaction test papers grew to 1.5 times as many, and resident-intruder test papers fell 6%.

Animal research papers that mention a behavioral test, by year, 2001 to 2026

The 2026 bar counts papers added to PubMed by November 8, 2026.

  • Papers in a full year
  • 2026 so far
Show the counts for each year
Papers by year
YearPapers
20012,404
20022,552
20032,746
20042,952
20053,189
20063,357
20073,469
20083,881
20093,948
20104,183
20114,606
20125,076
20135,516
20145,631
20155,582
20165,758
20175,880
20186,100
20196,182
20206,461
20216,786
20226,374
20235,863
20246,139
20257,115
2026 (part year)6,543

Papers that mention a test fell from 6,786 in 2021 to 5,863 in 2023, then rose to 7,115 in 2025. Papers that PubMed indexed under the MeSH heading Animals also fell and rose, from 200,221 in 2021 to 156,447 in 2023 and 235,479 in 2025. We take each paper's species from PubMed's indexing whenever it names one, so part of this dip may come from the indexing rather than from fewer studies.

Tests that grew the most

Of the tests with 100 or more papers in 2021 to 2025, these 6 grew the most since 2011 to 2015.

  • 2011 to 2015
  • 2021 to 2025
Splash test19 papers in 2011 to 2015, 132 in 2021 to 2025
6.9 times
Novel tank test46 papers in 2011 to 2015, 245 in 2021 to 2025
5.3 times
Three-chamber sociability test71 papers in 2011 to 2015, 339 in 2021 to 2025
4.8 times
Touchscreen operant tasks46 papers in 2011 to 2015, 146 in 2021 to 2025
3.2 times
Grip strength459 papers in 2011 to 2015, 1,354 in 2021 to 2025
2.9 times
Y-maze684 papers in 2011 to 2015, 1,988 in 2021 to 2025
2.9 times

Tests that fell the most

Of the tests with 100 or more papers in 2011 to 2015, these 6 fell the most by 2021 to 2025.

  • 2011 to 2015
  • 2021 to 2025
Step-down avoidance199 papers in 2011 to 2015, 97 in 2021 to 2025
51% fewer
Rotational behavior (rotometer)210 papers in 2011 to 2015, 110 in 2021 to 2025
48% fewer
Drug discrimination120 papers in 2011 to 2015, 65 in 2021 to 2025
46% fewer
Paw pressure test128 papers in 2011 to 2015, 70 in 2021 to 2025
45% fewer
Five-choice serial reaction time task174 papers in 2011 to 2015, 103 in 2021 to 2025
41% fewer
Radial arm maze361 papers in 2011 to 2015, 225 in 2021 to 2025
38% fewer

Which species the papers used

Among papers that mention a test, rats led every year from 2001 to 2019 and mice every year since 2020. In 2025, 3,862 papers used mice and 2,799 used rats. Mice still lead when we drop the species found by text mining and count only papers the National Library of Medicine indexed: 3,143 used mice and 2,284 used rats in 2025.

Papers that mention a behavioral test in mice and in rats, by year, 2001 to 2025

  • Mouse
  • Rat
Show the counts for each year and species
Papers by year, mice and rats
YearMouseRat
20017001,516
20027321,559
20038561,687
20049491,809
20051,0201,955
20061,1501,949
20071,2222,044
20081,4262,226
20091,5012,197
20101,6482,335
20111,8492,605
20122,0912,729
20132,3292,954
20142,3103,030
20152,3492,928
20162,5422,911
20172,6242,931
20182,7472,937
20192,8592,954
20203,0433,005
20213,3532,998
20223,1422,791
20232,9962,444
20243,2092,548
20253,8622,799

Papers from 2021 to 2025 that use each species

Each bar is a share of the 32,277 papers.

Mouse
16,562 papers 51%
Rat
13,580 papers 42%
Other animals
2,100 papers 7%
Zebrafish
651 papers 2%
Fruit fly
295 papers 1%
Nonhuman primate
188 papers 1%

“Other animals” counts animal research papers that use none of the species above, such as studies in guinea pigs, hamsters, rabbits, pigs or worms.

How we counted

A count here is the number of papers whose title or abstract mentions a test. It is not a count of experiments, labs or animals.

What counts as mentioning a test

A paper mentions a test when its title or abstract holds one of the test's search terms as whole words, such as “Morris water maze” or “water maze”. Some terms also name other things, so these known cases do not count toward the test they seem to name:

  • tail suspension used to unload the hind limbs, which is not the tail suspension test
  • a 12-hour light-dark cycle, which is not the light-dark box
  • a shuttle box used for passive avoidance, which counts as passive avoidance, not active avoidance

A radial arm water maze or a Cincinnati water maze does not count as a Morris water maze. Each test's search terms are in the test list download.

Other animals make up 45% of nest building papers. For tests like these, check the species shares in the table before reading the count as use in mice or rats.

We started from the behavioral tests our store lists, counting a variant such as an automated Y-maze under its main test. We added 20 well-known tests the store does not list, such as marble burying and the formalin test. In the test list download, these 20 have no store labels. Every test stays on the list, even one with few papers.

A paper that mentions a test only in its methods is missed, so every count runs low.

Which papers count as animal research

A paper counts when the National Library of Medicine's subject indexing (MeSH) names an animal species. When MeSH names no species, as for a paper PubMed has not indexed, the species come from PubTator 3, NCBI's text-mining service, if it finds one in the title or abstract.

From 2021 to 2025, 54,370 papers mentioned a test. We set aside 19,287 that studied only people and 2,806 with no species found, which leaves 32,277. PubTator 3 supplied the species for 6,460 of them.

Of the 962,474 papers the library indexed as animal research from 2021 to 2025, 25,817 (about 3%) mention one of these tests. The library's yearly totals on this page count papers indexed with the MeSH heading Animals, from the same PubMed files.

Which years count

Most counts on this page cover papers published from 2021 to 2025. The yearly series runs from 2001, and 2026 holds papers to November 8, 2026. Each paper counts in the year PubMed gives it.

Which publication types count

Errata, retraction notices, expressions of concern and preprints are left out, so a preprint and its journal version count once. Reviews are counted, and each test's review count is in the test list download.

Check 100 matches yourself

We drew 100 matches at random from the papers of 2021 to 2025. The random sample download gives each one with the text around it and a link to the paper, so you can judge whether the search term named the test. Because the 100 were drawn at random from all matches, the most-mentioned tests fill most of the sample, and rare tests appear rarely or not at all.

Sources and terms of use

Every number comes from dated public files loaded into ConductScience's research database, not from a live search. The “Every counted paper” download lists the papers behind each count.

The four public sources and their terms of use
SourceWhat it givesTerms of use
PubMedTitles, abstracts, years, subject indexing and publication types, from the yearly and daily filesCourtesy of the U.S. National Library of Medicine
PubTator 3Species and diseases it finds in the title or abstract, for papers the library has not indexed, with the diseases in the downloadsA U.S. Government work, free to use
iCiteCitation counts, in the downloadsCC BY 4.0, from the NIH Office of Portfolio Analysis
NIH RePORTERLinks from papers to NIH projects, in the downloadsPublic domain
The files we read, with their sizes and checksums
The files behind every count
FileSize in bytesSHA-256 checksum, or the records it holds
1,306 PubMed yearly files, combined November 7, 202635,813,134,336Records to August 31, 2026
387 PubMed daily update files, combined November 9, 20262,849,685,504Records to November 8, 2026
PubTator 3 concepts, dated October 6, 20266,138,750,4625a4254321d98daad0e6f35dda44410eade91b289466e6c1a146fb4b9645718cc
PubMed grant lists, dated November 6, 2026215,877,12239b1d9d845447564f93c2929e8d471aadc92f779915bf8f0058210dfaf754c7a
iCite citation counts, dated November 5, 2026340,402,664b417e4eb8a56f37497f299a4cc78e3406db02be19e9733a863657fb426bc368b
46 NIH RePORTER link tables, one per year, dated November 1, 202665,710,918Each file’s checksum is in the “All numbers” download

Downloads

Released November 10, 2026. CSV files open in any spreadsheet, and the compressed one needs unzipping first.

How to cite

APA

ConductScience. (2026, November 10). Behavioral tests in published research [Data set]. https://conductscience.com/data/behavioral-tests

BibTeX

@misc{conductscience_behavioral_tests_2026,
  author = {{ConductScience}},
  title = {Behavioral tests in published research},
  year = {2026},
  month = nov,
  url = {https://conductscience.com/data/behavioral-tests},
  note = {Data set, released November 10, 2026},
}

Release history

Releases of this data
ReleasedWhat changed
November 10, 2026We added a page for each of the 72 tests with 30 or more papers from 2021 to 2025, and for 22 disease models. We narrowed the search terms for 11 tests that also matched other meanings, such as cat hair in allergy studies. PubTator 3 now supplies a species or disease model only when it finds one in the title or abstract, the text we search. Labels from some papers' full text had made most disease models appear to fall in 2024. We renamed chronic stress to psychological stress, the name of the MeSH heading behind it, which also covers acute stress.
November 10, 2026First release: 90 tests, PubMed to November 8, 2026

Apparatus for 68 of the 90 tests

Our store sells apparatus for the Morris water maze, the open field, the novel object recognition, the forced swim test, the elevated plus maze and 63 more.

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