Full APO Long Working Distance Infinity Plan Apochromatic Microscope Objective Lens 2X 5X 10X 20X 50X 100X
Complete set of six infinity-corrected apochromatic microscope objectives (2X-100X) with numerical apertures from 0.055-0.8, featuring plan correction and long working distance design for high-resolution optical microscopy.
Louise Corscadden, PhD
Neuroscience · ConductScience
Ask Louise about Full APO Long Working Distance Infinity Plan Apochromatic Microscope Objective Lens 2X 5X 10X 20X 50X 100X fit, setup, configuration, or quote prep.
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The ConductScience Full APO Long Working Distance Infinity Plan Apochromatic Microscope Objective Lens Set provides a complete magnification range from 2X to 100X with corresponding numerical apertures from 0.055 to 0.8. These infinity-corrected apochromatic objectives are designed for high-resolution optical microscopy applications requiring color correction across the visible spectrum and extended working distances for specialized sample configurations.
Each objective in this six-lens set features plan correction for flat-field imaging and apochromatic design for minimized chromatic aberration. The long working distance configuration accommodates thicker specimens, coverslips, or specialized sample chambers while maintaining optical performance. Compatible with standard infinity-corrected microscope systems using 160mm tube length correction.
How It Works
Apochromatic objectives utilize specialized optical glass combinations and fluorite elements to correct chromatic aberration across the entire visible spectrum. Unlike achromatic objectives that correct for two wavelengths, apochromatic designs bring three or more wavelengths to the same focal plane, eliminating color fringing and providing superior image quality for color-critical applications.
The infinity-corrected optical design forms a parallel light bundle between the objective and tube lens, allowing insertion of additional optical elements such as filters, polarizers, or DIC prisms without affecting image quality. Plan correction flattens the field of view across the entire image plane, eliminating field curvature artifacts common in simpler objective designs.
Long working distance configuration increases the distance between the front lens element and specimen, achieved through modified optical design that maintains numerical aperture while providing clearance for specialized sample preparations, heating stages, or micromanipulation equipment.
Features & Benefits
Product Type
- Microscope
Microscope Type
- General Purpose
Application
- General Laboratory
Skill Level
- Intermediate
Brand
- ConductScience
Research Domain
- Cell Biology
- Clinical Diagnostics
- Developmental Biology
- Histopathology
- Materials Science
- Microbiology
Weight
- 1.9 kg
Dimensions
- L: 4.9 mm
- W: 6.6 mm
- H: 6.6 mm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Magnification Range Coverage | Complete 2X-100X range in six objectives | Often sold as individual objectives or incomplete sets | Provides complete magnification coverage without gaps, ensuring optimal objective selection for any application |
| Numerical Aperture Optimization | Progressive NA from 0.055 to 0.8 matched to magnifications | Entry-level sets may have suboptimal NA values | Maximizes resolution and light-gathering capacity at each magnification for superior image quality |
| Optical Correction Type | Full apochromatic correction with plan field flattening | Many objective sets use achromatic correction only | Eliminates chromatic aberration across visible spectrum and provides flat-field imaging for accurate measurements |
| Working Distance Design | Long working distance configuration across all magnifications | Standard working distance is more common | Accommodates thick specimens and specialized sample chambers while maintaining optical specifications |
| Infinity Correction | Infinity-corrected optical design for all objectives | Some budget sets still use finite tube length correction | Allows insertion of additional optical components and ensures compatibility with modern microscope systems |
This objective set combines apochromatic color correction, plan field flattening, and long working distance design across a complete magnification range with optimized numerical apertures for each magnification level.
Practical Tips
Use calibration slides with known features to verify parfocal adjustment and optical performance across all magnifications.
Why: Ensures accurate measurements and consistent image quality when switching between objectives.
Clean objectives immediately after use and store in protective cases to prevent dust accumulation and coating damage.
Why: Maintains optimal optical transmission and prevents expensive repairs from contaminated or scratched lens surfaces.
Match condenser numerical aperture to objective NA and adjust illumination intensity appropriately for each magnification.
Why: Optimizes resolution and contrast while preventing photodamage to specimens and photobleaching in fluorescence applications.
If parfocal adjustment is lost, start with the lowest magnification objective and readjust the nosepiece height incrementally.
Why: Systematic adjustment prevents damage to objectives and ensures proper working distance maintenance.
Verify field flatness across the entire image area when documenting or making measurements, especially with plan-corrected objectives.
Why: Ensures measurement accuracy and image quality consistency across the field of view for quantitative applications.
Always approach specimens slowly when focusing with high numerical aperture objectives to prevent collision damage.
Why: High NA objectives have very short working distances and expensive lens elements that can be easily damaged by contact with samples.
Allow objectives to temperature equilibrate before critical measurements, especially when moving between different environmental conditions.
Why: Temperature changes can affect focus position and optical performance due to thermal expansion of optical elements.
Setup Guide
What’s in the Box
- 2X apochromatic objective lens (NA 0.055)
- 5X apochromatic objective lens (NA 0.15)
- 10X apochromatic objective lens (NA 0.3)
- 20X apochromatic objective lens (NA 0.45)
- 50X apochromatic objective lens (NA 0.65)
- 100X apochromatic objective lens (NA 0.8)
- Protective storage case (typical)
- Lens cleaning cloth (typical)
- User manual with specifications (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering defects in materials and workmanship, with technical support for optical performance questions and application guidance.
Compliance
References
Background reading relevant to this product:
What microscope systems are compatible with these infinity-corrected objectives?
These objectives are compatible with any infinity-corrected microscope system using standard RMS threading. Verify your microscope tube length is set to infinity (∞) and includes appropriate tube lens for proper optical correction.
How does the long working distance affect optical performance compared to standard objectives?
Long working distance design maintains the specified numerical apertures while providing increased clearance between objective and specimen. This may result in slightly reduced light transmission compared to equivalent standard working distance objectives.
Can these objectives be used for oil immersion applications?
The 100X objective with NA 0.8 is designed for dry applications. For oil immersion work requiring higher numerical apertures (typically NA >1.0), consult product datasheet for available oil immersion variants.
What is the field of view coverage with plan correction?
Plan correction ensures sharp focus across the entire field of view for each objective. Actual field diameter depends on your microscope's tube lens and eyepiece configuration - consult your microscope specifications.
How should these apochromatic objectives be maintained for optimal performance?
Clean lens surfaces only with appropriate lens tissue and cleaning solutions. Store in protective cases when not in use, and avoid touching optical surfaces. Regular inspection for coating damage or contamination is recommended.
Are these objectives parfocal when properly adjusted?
Yes, when properly installed and adjusted, these objectives should maintain approximate focus when switching between magnifications, though fine focus adjustment may still be needed for critical work.
What illumination requirements are needed for optimal performance?
Configure Köhler illumination with condenser numerical aperture matched to each objective. Ensure adequate light intensity particularly for higher magnifications, and adjust field diaphragms appropriately for each objective.
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