Large Horizontal Autoclave
Large 450L horizontal steam autoclave with automatic door and jacketed chamber design for high-throughput laboratory and facility sterilization.
Features 7-inch LCD touch screen, pulse vacuum system, and programmable sterilization cycles. Requires three-phase power supply.
Louise Corscadden, PhD
Director of Science · ConductScience
Ask Louise about Large Horizontal Autoclave fit, setup, configuration, or quote prep.
Already working with us? Sign in to connect this with My Scientist.
Key Specifications
Full details →- Model fit
- Configured during quote
- SKU family
- BIO-0150
- Sizing
- 205.0 x 126.0 x 131.4 cm
- Ordering
- Online checkout and quote request available
- Category
- Autoclaves
- Build notes
- S30408
Updated description to note 200L capacity model correction
How It Works
Steam sterilization operates through moist heat transfer at elevated temperature and pressure, causing irreversible denaturation of microbial proteins and nucleic acids. The autoclave generates saturated steam at 0.8MPa through an integrated steam generator, which is distributed throughout the 450L chamber volume. The water circulating vacuum system removes air prior to steam injection, ensuring complete steam penetration and eliminating cold spots that could compromise sterilization efficacy.
Temperature control is maintained through automated monitoring with 0.1°C precision, while pressure regulation ensures optimal steam conditions throughout the sterilization cycle. The horizontal chamber configuration facilitates loading of large equipment and allows gravitational condensate drainage during the process.
Following the sterilization phase, controlled depressurization and optional drying cycles (0-99 minutes) prepare materials for immediate use or storage. The LCD touchscreen interface provides cycle programming, real-time monitoring, and documentation of critical process parameters.
Features & Benefits
Automation Level
- semi-automated
Door
- Automatic
Designed Pressure
- Inner chamber: -0.1/0.3MPa; Jacket: 0.3MPa
Working Pressure
- 0.21MPa
Designed Temp.
- 150℃
Max Working Temp.
- 134℃
Temp. Precision
- 0.1℃
Built-In Steam Generator
- 0.8MPa
Sterilization Time
- 0~99min
Drying Time
- 0~99min
Vacuum system
- Water circulating vacuum pump
Chamber Material
- S30408
Chamber Size(W*D*H)
- 672*1000*672mm
Brand
- ConductScience
Research Domain
- Cell Biology
- Clinical Diagnostics
- Environmental Monitoring
- Food Science
- Microbiology
- Pharmaceutical QC
Capacity
- 450L
Display Type
- 7-inch LCD touch screen
Weight
- 1035.0 kg
Dimensions
- L: 205.0 mm
- W: 126.0 mm
- H: 131.4 mm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Chamber Capacity | 450L internal volume | Entry-level models typically offer 100-200L capacity | Reduces the number of sterilization cycles required for large laboratory operations, improving overall productivity. |
| Steam Generation | Integrated steam generator at 0.8MPa | Many units require external facility steam supply | Provides independent operation with consistent steam quality regardless of facility steam availability. |
| Door Operation | Automatic door with safety interlocks | Manual door operation common in smaller units | Reduces operator exposure to hot surfaces and ensures consistent seal pressure for reliable sterilization. |
| Temperature Control | 0.1°C precision control | Standard models often provide ±1°C control | Enables precise validation documentation and maintains tight process control for critical applications. |
| User Interface | 7-inch LCD touchscreen | Basic digital displays or analog controls | Provides intuitive programming and comprehensive cycle documentation capabilities. |
| Vacuum System | Water circulating vacuum pump | Gravity displacement or basic vacuum systems | Ensures complete air removal for reliable steam penetration through complex loads and wrapped materials. |
This autoclave combines large capacity processing with integrated steam generation and precision control systems. The 450L chamber with automatic door operation and touchscreen interface provides efficient sterilization for high-volume laboratory applications requiring validated processing documentation.
Practical Tips
Verify temperature and pressure sensor accuracy quarterly using calibrated reference instruments and maintain calibration records.
Why: Ensures sterilization cycle validity and supports regulatory compliance documentation requirements.
Inspect door seals weekly for wear or damage and replace immediately if compromised to maintain vacuum and pressure integrity.
Why: Prevents cycle failures and ensures consistent sterilization conditions throughout the chamber volume.
Allow adequate spacing between items in loads to ensure steam circulation and avoid overloading the chamber capacity.
Why: Promotes uniform heat and steam distribution, preventing cold spots that could compromise sterilization efficacy.
If vacuum system fails to reach proper levels, check water supply pressure and circulation pump operation before cycling.
Why: Inadequate vacuum prevents complete air removal, leading to sterilization failures and potential reprocessing requirements.
Document all cycle parameters including time, temperature, pressure, and load details for each sterilization run.
Why: Provides traceability for validation requirements and helps identify trends in equipment performance over time.
Never attempt to open the door during or immediately after cycles - allow pressure equalization and cooling as indicated by the control system.
Why: Prevents steam burns and pressure-related injuries while protecting chamber integrity and door seal mechanisms.
Use biological indicators periodically to validate sterilization efficacy, especially after maintenance or equipment modifications.
Why: Confirms that sterilization conditions are achieving the required log reduction of microbial contamination.
Descale the steam generator according to water quality conditions and usage frequency to maintain efficient steam production.
Why: Prevents mineral buildup that can reduce steam quality and generation efficiency, leading to cycle inconsistencies.
Setup Guide
What’s in the Box
- Large horizontal autoclave main unit
- Automatic door system with safety interlocks
- 7-inch LCD touchscreen control panel
- Integrated steam generator
- Water circulating vacuum pump
- Chamber loading accessories (typical)
- Installation hardware and connections (typical)
- Operating manual and validation documentation (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering parts and labor with comprehensive technical support for installation, operation, and maintenance guidance.
Compliance
What is the maximum load volume and weight capacity for a single sterilization cycle?
The 450L chamber (672×1000×672mm internal dimensions) accommodates substantial loads, but specific weight limits depend on load distribution and chamber rack configuration - consult product datasheet for maximum load specifications.
How is sterilization efficacy validated and documented?
The system provides digital cycle recording through the LCD interface, with temperature and pressure monitoring at 0.1°C precision. Biological indicator testing with spore preparations should be performed according to institutional protocols.
What utilities are required for installation and operation?
The autoclave requires electrical power, water supply for the integrated steam generator, and drainage connections. Specific utility requirements including voltage, water pressure, and flow rates should be verified from installation specifications.
Can the autoclave process wrapped materials and complex instruments?
Yes, the water circulating vacuum system removes air prior to steam injection, ensuring steam penetration through wrapping materials and into complex device geometries.
What maintenance procedures are required for reliable operation?
Regular maintenance includes steam generator descaling, vacuum pump servicing, door seal inspection, and periodic calibration verification. Specific maintenance intervals depend on usage frequency and water quality.
How does the horizontal design compare to vertical autoclaves?
The horizontal configuration facilitates loading of large equipment and wheeled carts while providing gravitational condensate drainage. This design is particularly suited for processing bulky laboratory equipment and large volume loads.
What cycle parameters can be customized for different load types?
The touchscreen interface allows programming of sterilization time (0-99 minutes), drying time (0-99 minutes), and temperature settings up to 134°C maximum, with pressure control according to load requirements.
What capacity models are available for the large horizontal autoclave?
The large horizontal autoclave is available in 200L capacity. Please verify capacity specifications as product information is updated regularly to reflect accurate model specifications.
What are the electrical requirements for the 450L horizontal autoclave?
This 450L model requires three-phase electricity (380V, 50Hz). Standard single-phase 110V power cannot support autoclaves above 120L capacity. Confirm your facility has three-phase electrical service before ordering.
Have a question about this product?
Have a question? Just ask.
Send it over and we'll email you a personalized answer — no call, no scheduling.
Prefer to talk it through?
Accessories
Enhance your setup with compatible accessories
Frequently Bought Together
