
Large Steam Autoclave Medical Sterilizing Machine
High-capacity vertical steam autoclave with jacketed chamber design for laboratory and medical sterilization applications, available in 146L and 200L configurations.
Requires three-phase power supply (380V, 50Hz).

Louise Corscadden, PhD
Director of Science · ConductScience
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Key Specifications
Full details →- Model fit
- Configured during quote
- SKU family
- BIO-0153
- Sizing
- 32.68 x 16.54 x 16.54 cm
- Ordering
- Online checkout and quote request available
- Category
- Autoclaves
- Build notes
- Confirm accessories, station layout, and support needs before purchase
BKQ series pulse vacuum sterilizer is an advanced high pressure steam sterilizer designed and manufactured according to the latest requirements of sterilization. Features superb chamber machining and welding process with acid-proof stainless steel construction.
How It Works
Steam sterilization operates on the principle of moist heat destruction of microorganisms through protein coagulation and enzyme inactivation. The autoclave generates saturated steam under pressure, creating temperatures above 100°C that penetrate materials and achieve reliable microbial kill rates. The jacketed chamber design maintains uniform temperature distribution by circulating steam through the outer jacket while the inner chamber holds the load.
During the sterilization cycle, air is displaced from the chamber through gravity displacement or vacuum extraction, allowing steam to contact all surfaces directly. The combination of temperature, pressure, and time parameters creates conditions that denature proteins and nucleic acids in bacterial spores, vegetative bacteria, viruses, and fungi. Temperature monitoring systems track the sterilization process to ensure adequate heat penetration throughout the load.
Features & Benefits
Brand
- ConductScience
Research Domain
- Cell Biology
- Clinical Diagnostics
- Environmental Monitoring
- Food Science
- Microbiology
- Pharmaceutical QC
Automation Level
- semi-automated
Chamber Type
- Jacketed
Designed Pressure (Inner)
- -0.1/0.3 MPa
Designed Pressure (Jacket)
- 0.3 MPa
Max Designed Temp
- 144°C
Working Pressure
- 0.25 MPa
Max Working Temp
- 139°C
Built-in Steam Generator
- 0.28 MPa
Available Capacities
- 146L
- 200L
146L Chamber Size
- 420×420×830mm
146L External Size
- 1290×1680×1050mm
146L Net Weight
- 425 kg
146L Gross Weight
- 525 kg
200L Chamber Size
- 600×600×670mm
200L External Size
- 1290×1680×1100mm
200L Net Weight
- 510 kg
200L Gross Weight
- 610 kg
Power Consumption
- 18KW
Power Supply
- 380V, 50Hz (three-phase required)
Weight
- 68.56 kg
Dimensions
- L: 32.68 mm
- W: 16.54 mm
- H: 16.54 mm
Available in multiple sizes: 146L, 241L, 352L, 655L, 810L, 999L, 1203L, and 1500L models. Choose based on your facility throughput requirements. Models available with single or double door configurations.
Used for sterilization of health care products and their accessories. Suitable for hospitals, clinics, medical facilities, research laboratories, and industrial applications requiring high-capacity sterilization.
Practical Tips
Verify temperature accuracy using calibrated thermocouples placed throughout the chamber load to ensure uniform heat distribution meets validation requirements.
Why: Temperature uniformity is critical for reliable sterilization and regulatory compliance in validated processes.
Perform weekly chamber cleaning with appropriate detergents and monthly inspection of door seals and gaskets for wear or damage.
Why: Regular cleaning prevents residue buildup that can interfere with steam circulation and compromise sterilization effectiveness.
Load items to allow adequate steam circulation between packages and avoid overloading the chamber beyond recommended capacity.
Why: Proper loading ensures steam can reach all surfaces and prevents cold spots that could result in sterilization failures.
Monitor heat-up times and investigate any significant increases in time to reach operating temperature as this may indicate heating element or insulation issues.
Why: Changes in heat-up performance can indicate equipment problems that could affect sterilization cycle reliability.
Maintain detailed records of cycle parameters, load contents, and any process deviations for each sterilization run.
Why: Comprehensive documentation supports process validation requirements and enables trend analysis for preventive maintenance.
Always allow adequate cooling time before opening the chamber and use appropriate personal protective equipment when handling hot sterilized items.
Why: Steam sterilization creates high temperatures and pressures that pose burn hazards if proper safety procedures are not followed.
Use appropriate sterilization indicators and biological indicators to verify cycle effectiveness according to your laboratory's quality assurance protocols.
Why: Chemical and biological indicators provide independent verification that sterilization parameters were achieved throughout the load.
Schedule periodic calibration of pressure and temperature monitoring systems according to manufacturer recommendations and regulatory requirements.
Why: Accurate monitoring system calibration ensures reliable process control and maintains validation status for critical sterilization cycles.
Setup Guide
Automatic door operation with motor drive. Colorful touch screen interface for easy operation. Multiple safety features including automatic pressure control and safety valve systems. Professional installation and training available.
What’s in the Box
- Large steam autoclave main unit
- Operating manual and documentation
- Power cable and electrical connections (typical)
- Steam supply fittings (typical)
- Temperature monitoring probes (typical)
- Installation hardware kit (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering parts and labor with comprehensive technical support for installation and operation guidance.
Compliance
Technical documentation available including specification sheets and operation manuals. Certifications and compliance documentation on file. ISO 17665-1 certified for steam sterilization processes.
Maintains temperature and pressure control from -0.1/0.25 Mpa to 0.25 Mpa working pressure. Maximum working temperature of 139C for safe sterilization. Unique vacuum noise-reduction system provides quiet operation.
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