
Self-pressurized Liquid Nitrogen Tank
Laboratory-scale self-pressurized liquid nitrogen storage tank for cryogenic preservation of biological samples and temperature-sensitive materials without external pressurization requirements.
| Automation Level | manual |
| 30 | 200 |
| 45 | 120 |
| 50 | 50 |
| 850 | 1310 |
| 2.5 | 1.2 |
The Self-pressurized Liquid Nitrogen Tank (BIO-0825) is a cryogenic storage system designed for laboratory-scale preservation of biological samples, cell lines, and temperature-sensitive materials. This tank utilizes internal pressure build-up from natural nitrogen evaporation to maintain delivery pressure without external pressurization systems, enabling consistent sample access while minimizing nitrogen loss through efficient insulation design.
The system provides controlled cryogenic storage for research applications requiring ultra-low temperature preservation, including long-term cell banking, enzyme storage, and biological specimen archiving. The self-pressurization mechanism eliminates the need for external pressure sources, reducing operational complexity while maintaining stable internal conditions for sample integrity.
How It Works
The self-pressurized liquid nitrogen tank operates on the principle of controlled evaporation and pressure management within a vacuum-insulated vessel. As liquid nitrogen naturally evaporates at the bottom of the tank, it creates gaseous nitrogen pressure that forces liquid nitrogen up through an internal dip tube to the dispensing valve. This eliminates the need for external pressure sources or pumping systems.
The tank's double-wall vacuum insulation minimizes heat transfer from the environment, reducing nitrogen evaporation rates while maintaining the pressure differential required for liquid delivery. Internal pressure regulation prevents over-pressurization through controlled venting mechanisms, ensuring consistent delivery pressure throughout the tank's operational cycle.
Temperature stratification within the tank is managed through internal design features that promote even cooling distribution while maintaining the liquid-gas interface necessary for pressure generation. This design enables extended holding times while preserving sample integrity through stable cryogenic temperatures.
Features & Benefits
Automation Level
- manual
30
- 200
45
- 120
50
- 50
850
- 1310
2.5
- 1.2
450
- 650
0.05
- 0.05
0.09
- 0.09
0.15
- 0.15
0-0.25
- 0-0.25
Brand
- ConductScience
Research Domain
- Analytical Chemistry
- Cancer Research
- Cell Biology
- Developmental Biology
- Materials Science
- Microbiology
- Pharmaceutical QC
Weight
- 29.98 kg
Dimensions
- L: 42.0 mm
- W: 43.6 mm
- H: 38.0 mm
Comparison Guide
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Pressure Generation Method | Internal evaporation-based self-pressurization | External gas cylinder or pump-based pressurization systems | Eliminates external infrastructure requirements and reduces operational complexity for routine laboratory use |
| Operating Pressure Range | 0.05 to 0.25 MPa operational range | Fixed pressure systems or limited pressure adjustment capability | Multiple pressure options accommodate different sample dispensing requirements and laboratory workflows |
| Capacity Configurations | Multiple capacity options with varying holding times | Fixed capacity models with limited size selection | Allows laboratory-specific sizing for optimal nitrogen utilization and storage efficiency |
| Startup Requirements | Natural pressure development through evaporation | Immediate pressurization with external gas supply | Reduces dependency on external gas supplies and associated delivery logistics |
This self-pressurized liquid nitrogen tank provides operational independence through internal pressure generation across multiple capacity and pressure configurations. The system offers simplified installation and operation compared to externally pressurized alternatives while maintaining flexible pressure ranges for various laboratory applications.
Practical Tips
Allow adequate time for pressure development before sample access, typically 2-4 hours after filling or major usage.
Why: Insufficient pressure results in poor liquid flow and inefficient nitrogen utilization during sample procedures.
Check pressure gauge calibration monthly and inspect relief valve operation quarterly for safety system integrity.
Why: Accurate pressure monitoring and functional safety systems prevent over-pressurization and ensure safe laboratory operation.
Verify delivery pressure consistency across different tank fill levels to establish optimal operating parameters.
Why: Pressure varies with liquid level and evaporation rate, affecting sample dispensing consistency throughout operational cycles.
Maintain minimum 3-meter clearance around pressure relief valve discharge points and ensure adequate room ventilation.
Why: Nitrogen gas displacement can create oxygen-deficient environments requiring proper ventilation design for personnel safety.
Monitor nitrogen consumption rates to establish refill schedules that maintain consistent sample storage temperatures.
Why: Temperature fluctuations during low nitrogen periods can compromise sample integrity and experimental reproducibility.
If liquid delivery stops, check internal pressure levels and allow additional time for pressure recovery before mechanical inspection.
Why: Low internal pressure is the most common cause of delivery issues and often resolves through natural pressure buildup.
Setup Guide
What’s in the Box
- Self-pressurized liquid nitrogen tank (main unit)
- Pressure gauge assembly
- Dispensing valve with connecting hardware
- Pressure relief valve (safety system)
- User manual and safety guidelines
- Warranty documentation (typical)
Warranty
ConductScience provides standard manufacturer warranty coverage for cryogenic storage systems including parts and technical support. Warranty terms cover manufacturing defects and operational performance under normal laboratory use conditions.
Compliance
References
Background reading relevant to this product:
How long does it take for the tank to develop sufficient pressure for liquid withdrawal after initial filling?
Pressure development typically requires 2-4 hours after initial filling as internal evaporation generates the pressure differential needed for liquid delivery through the dip tube system.
What factors affect the holding time and nitrogen loss rate in self-pressurized tanks?
Holding time depends on ambient temperature, frequency of use, tank insulation efficiency, and internal pressure settings. Frequent opening and higher ambient temperatures increase evaporation rates.
Can the tank pressure be adjusted for different laboratory applications?
Operating pressure is determined by internal evaporation rates and relief valve settings. Consult product datasheet for pressure adjustment capabilities and recommended operating ranges.
What safety considerations are important for laboratory installation?
Ensure adequate ventilation for nitrogen gas dispersal, maintain clearance around pressure relief valves, and establish protocols for pressure monitoring and emergency valve operation.
How does sample access frequency affect tank performance?
Frequent opening reduces internal pressure and increases nitrogen consumption. Plan sample access to minimize opening frequency and duration for optimal efficiency.
What maintenance is required for the pressure regulation system?
Regular inspection of pressure gauge accuracy, relief valve function, and valve sealing integrity. Professional servicing may be required for pressure system components.
How do self-pressurized tanks compare to externally pressurized systems?
Self-pressurized tanks eliminate external gas cylinder requirements but may have longer startup times and pressure-dependent delivery rates compared to constant-pressure external systems.




