Science

Reference to this article: ConductScience, Fiber Photometry: Definition & Equipment Needed (2022). doi.org/10.55157/CS20221111 Fiber photometry uses spectroscopy to monitor the cellular dynamics in the cell or organ of interest in vivo. It uses photons from the light of a specified wavelength to trigger cells at the region of interest, enabling researchers to monitor the dynamics […]

Patch clamp electrophysiology is an experimental technique that reads into electrical events in cells. The electric potential between the cell membrane reveals the workings of cell membranes and ion channels, a group of proteins that detect and control the movement of ions in and out of the cells.[1]    The technique was developed through a […]

Introduction Cells are building blocks of organisms. Thus, understanding the whole metabolic processes and dynamics of organisms requires us to look clearly inside cells. Advanced microscopy technologies combined with protein labeling approaches have enabled scientists to understand cellular processes and investigate the dynamic processes in organisms with better resolution and sensitivity.[1] The quality optical conditions […]

Tissue slices represent the organs or intact tissue for in vitro studies such as biochemical analyses, live-cell imaging, electro-physiological, and pharmacological studies. Theoretically, tissue slices are short-lived and should be used immediately. In practice, tissue slices can be temporarily maintained in cold storage or cryopreserved to reduce or stop their metabolic activities, thus extending their […]

What is Brain Slice? Electrophysiology refers to studying the electrical properties of biological cells and tissues. Thus, brain or neuronal electrophysiology pertains to a branch of electrophysiology that deals with measuring electric current or voltage changes in one neuronal cell, a brain slice, or the whole brain. The methodology was first introduced by Yamamoto and […]

Tissue slices are viable tissue sections used as in vitro representatives of their originating tissues or organs for metabolic studies, including live-cell imaging, electrophysiological analysis, and pharmacological studies. Theoretically, slices of tissue or organ are immediately used for their intended examinations. However, this is not usually realistic. Tissue slices are prepared once but often examined […]

Introduction Today, we all — biologists or not — have an idea about how the brain functions. The advanced techniques and lab procedures have made the knowledge accessible and unlocked many mysteries related to brain functioning. One such procedure is brain slicing. It’s a process in which parts of the brain are divided using advanced […]

Tissue slices are sections of tissue cut from larger pieces or extracted organs and treated in a medium to gain insights into the working of the originating tissue or organ. Unlike sections for histological studies, tissue slices for metabolic studies are not fixed and preserved. Rather, they are prepared immediately after the excision or after […]

What Are Electrophysiology Chambers? Electrophysiology chambers are designed to study tissue electrophysiology in preclinical research. These chambers can be used for tissue slices in neuropharmacological and toxicological animal models in preclinical research, especially in rodents like rats and guinea pigs. Electrophysiological recordings can be taken simultaneously from tissue slices using electrophysiology chambers, which allows researchers […]