Subcellular

Nuclear & RNA Foci

Count the spots inside every nucleus.

Segment nuclei, then detect and quantify intranuclear puncta as a per-nucleus readout: RNA foci, DNA-damage foci, or FISH spots. Track foci count, intensity, and area to read target engagement across a dose-response, the same way ConductRNA reads nuclear-foci dissolution.

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Immunofluorescence micrograph: red DNA-damage (53BP1) foci as discrete puncta inside blue-stained cell nuclei, with the green tubulin cytoskeleton around them.
53BP1 DNA-damage foci — Velichko Artem, CC BY 4.0, via Wikimedia Commons
Per nucleus
Foci readout
Count + intensity
& area
Dose-ready
Batch export
Outputs

What gets measured

Foci are assigned to their parent nucleus, so every metric is both per-cell and aggregated across the population.

01

Per nucleus

  • Foci count
  • Mean foci intensity
  • Total foci area
02

Population

  • Foci-positive fraction
  • Foci-per-nucleus distribution
03

Signal

  • Integrated intensity
  • Background-corrected
04

Screening

  • Per-well aggregate
  • Dose-response ready
Workflow

How It Works

1

Load

Load a fluorescence image or a folder of dose points.

2

Segment nuclei

AI separates touching nuclei to define each per-cell region.

3

Detect foci

Intranuclear puncta are detected and assigned to their parent nucleus.

4

Export

Download per-nucleus foci counts, intensities, and per-well aggregates.

Explore More

Related Applications

Nuclei (Fluorescence)

Nuclei (Fluorescence)

Fluorescence-based nuclei detection and quantification

Cell Count (Fluorescence)

Cell Count (Fluorescence)

Precise cell counting using fluorescent markers

Hematoxylin-Stained Nuclei

Hematoxylin-Stained Nuclei

Detect nuclei in H&E histology sections

Ready to run this assay on your own images?

Request a demo and see ConductVision Image analyze your nuclear & rna foci data.

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