Tracked Object · Organelles

Organelle Tracking

Follow mitochondria and lysosomes through the cell.

Organelles are one of the four object classes ConductVision Image tracks. Segment and link mitochondria, lysosomes, endosomes, or autophagosomes frame by frame to quantify intracellular trafficking: how far each cargo travels, how directed that travel is, and where it stalls. Directionality separates directed transport from confined, random motion on the manual’s own scale.

Fluorescence micrograph: the red filamentous mitochondrial network of a living cell surrounding a blue-stained nucleus, the kind of labeled organelle cargo the engine tracks.
Mitochondria in living HeLa cells — 8x57is, CC BY-SA 4.0, via Wikimedia Commons
Organelles
Tracked object class
Directionality
Directed vs confined
Per-frame
Full X / Y track export
Outputs

Trafficking readouts

The exported columns come straight from the tracking tables. Run length, pause frequency, and transport direction are computed from the exported per-frame track rather than emitted natively.

01

Motion (exported)

  • TotalPathLength
  • NetDisplacement
  • Directionality
02

Per frame (exported)

  • Frame
  • X
  • Y
  • ObjectId
03

Temporal (exported)

  • AvgSpeed
04

Derived downstream

  • Run length
  • Pause frequency
  • Anterograde vs retrograde
Workflow

How It Works

1

Load video

Point a tracking protocol at a time-lapse of labeled organelles.

2

Set cargo diameter

Draw one average organelle so detection calibrates to sub-cellular scale.

3

Track & link

Each cargo is detected and linked across frames into a continuous trajectory.

4

Export

Download motion tables plus the full per-frame X / Y track for run and pause analysis.

Ready to track cargo in your own time-lapse?

Request a demo and see ConductVision Image trace organelle trafficking frame by frame.

Request a Demo