Migration, wound healing, and angiogenesis image analysis.
Measure movement, closure, and network formation from assay images and time-lapse series.
What this page covers
Use this page for assays where the biological question is movement, closure, invasion, or network formation rather than a simple object count.
Talk through your images
Bring the time points, imaging mode, and closure or network endpoint. We will determine whether a standard wound, tube, or custom migration workflow fits.
Request a meetingMeasurements
- Wound area
- Closure percent
- Migration rate
- Tube length
- Branch points
- Node count
Review outputs
- Time-point overlays
- Closure curves
- Network skeletons
- Per-condition exports
Image inputs, QC, and outputs
Use this section to decide whether the page matches your assay before requesting a meeting. The goal is to preserve the biological endpoint while making the image analysis repeatable, reviewable, and exportable.
Image inputs
Migration and invasion analysis starts with representative images that match the endpoint your lab reports. Include controls and edge cases so thresholds can be set against real biological variation, not only ideal fields.
- Primary measurements: Wound area, Closure percent, Migration rate, and Tube length
- Matched positive and negative controls when available
- Consistent magnification, channel order, plate layout, or time-point labels
QC and validation
Each workflow should leave a visible trail from raw image to measurement. Review masks, thresholds, and flagged fields before exporting the final table.
- Raw image and overlay review before batch export
- Locked settings for repeated plates, stains, or time points
- QC flags for dim signal, merged objects, uneven background, or failed segmentation
Outputs for analysis
ConductVision should return both visual evidence and structured data so the result can be checked, summarized, and reused in downstream statistics.
- Typical outputs: Time-point overlays, Closure curves, Network skeletons, and Per-condition exports
- Per-image, per-cell, per-object, or per-well tables where relevant
- CSV exports for Prism, Excel, R, Python, or LIMS handoff
Workflows to start from
Open an existing ConductVision page when the workflow already exists, or request a meeting when your assay needs custom thresholds, outputs, or validation rules.
Scratch and wound assay
Wound area, closure percent, migration rate, and edge masks from phase or brightfield images.
Angiogenesis and tubes
Tube length, branch points, nodes, and mesh counts from network-formation assays.
Percent area
Area and coverage measurements for invasion, matrix, or staining fields.
Related Life Science pages
Use these pages to move between adjacent assays, specialist microscopy workflows, and the full Life Science atlas.
Request a Life Science meeting
Share your assay, representative images, and the measurement you need so the team can map the closest ConductVision workflow.
3D culture
Analyze organoid, spheroid, and aggregate growth alongside migration endpoints.
Life Science atlas
Return to the full biology application atlas for cells, assays, tissue, microscopy, and morphology.
Frequently asked questions
These answers cover the practical questions labs usually ask before sending example images or requesting a ConductVision workflow review.
What images work best for migration and invasion analysis?
Use representative images from the same microscope, scanner, plate reader, or camera setup used in the study. The most useful examples include controls, typical fields, difficult fields, and any cases that affect Wound area, Closure percent, Migration rate, and Tube length.
Can ConductVision handle custom migration and invasion endpoints?
Bring the time points, imaging mode, and closure or network endpoint. We will determine whether a standard wound, tube, or custom migration workflow fits. ConductScience can confirm whether an existing workflow fits or define custom segmentation, thresholding, review, and export rules for the assay.
What results are usually exported from this workflow?
The expected deliverables include Time-point overlays, Closure curves, and Network skeletons, plus structured tables for downstream analysis. The page also lists the specific measurements and review outputs that are most relevant to this application.
Find out if ConductVision fits your migration and invasion workflow
Send representative images, assay details, and the measurement endpoint. ConductScience can confirm the closest existing workflow or scope a custom analysis path.
