ConductVision · Behavioral Analysis

Novel Tank Diving Test

Quantify anxiety-like behavior through vertical exploration and bottom-dwelling in zebrafish.

ZebrafishAnxietyAuto Export
ConductVision / Novel Tank Diving Test
Recording / Trial 3fish tracked
Bottom Dwelling Time42%
Latency to Upper Half7.2s
Transitionsauto

Key Parameters

Metrics automatically extracted by ConductVision.

Bottom Dwelling Time

Duration spent in the lower third of the tank — primary anxiety index

Latency to Upper Half

Time to first cross into the upper half of the tank

Transitions

Number of crossings between top and bottom zones

Freezing Bouts

Immobility episodes lasting more than two seconds

Erratic Movements

Sudden direction changes exceeding angular velocity threshold

Total Distance

Cumulative swim path length across the trial

What is the Novel Tank Diving Test?

The Novel Tank Diving Test is the zebrafish equivalent of the rodent open-field test. When placed in an unfamiliar tank, zebrafish exhibit a characteristic diving response — spending most of the initial minutes near the bottom. Over time, exploration of the upper regions increases. Prolonged bottom-dwelling, freezing, and erratic swimming indicate elevated anxiety.

ConductVision divides the tank into configurable horizontal zones and tracks vertical position in real time. The software automatically scores bottom-dwelling time, latency to upper exploration, and freezing bouts — enabling high-throughput anxiolytic drug screening and genetic studies of stress-related behavior.

Ready to automate your behavioral analysis?

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