Bottom Dwelling Time
Duration spent in the lower third of the tank — primary anxiety index
Quantify anxiety-like behavior through vertical exploration and bottom-dwelling in zebrafish.
Metrics automatically extracted by ConductVision.
Duration spent in the lower third of the tank — primary anxiety index
Time to first cross into the upper half of the tank
Number of crossings between top and bottom zones
Immobility episodes lasting more than two seconds
Sudden direction changes exceeding angular velocity threshold
Cumulative swim path length across the trial
The Novel Tank Diving Test is the zebrafish equivalent of the rodent open-field test. When placed in an unfamiliar tank, zebrafish exhibit a characteristic diving response — spending most of the initial minutes near the bottom. Over time, exploration of the upper regions increases. Prolonged bottom-dwelling, freezing, and erratic swimming indicate elevated anxiety.
ConductVision divides the tank into configurable horizontal zones and tracks vertical position in real time. The software automatically scores bottom-dwelling time, latency to upper exploration, and freezing bouts — enabling high-throughput anxiolytic drug screening and genetic studies of stress-related behavior.
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