Research data

Brain techniques in behavioral research

From 2021 to 2025, 3,620 animal research papers mentioned a behavioral test and one of these 16 brain techniques, from viral vectors and optogenetics to EEG and microdialysis.

3,620 animal research papers from 2021 to 2025 mentioned a test and one of these brain techniques
11% of the 32,277 animal research papers from 2021 to 2025 that mention a test also mention one of these brain techniques
16 brain techniques counted, 11 with a page of their own

Every brain technique we count

Each bar counts the animal research papers from 2021 to 2025 that mention the brain technique and at least one of the 90 behavioral tests. Under each name are the three tests mentioned most in its papers, with the share of its papers that mention each. Each brain technique with 100 or more such papers has a page of its own.

Intracerebroventricular (ICV) injectionMorris water maze 38% · Novel object recognition 18% · Passive avoidance 14%
706 papers
AAV vectorsMorris water maze 19% · Open field 13% · Fear conditioning 10%
546 papers
Chemogenetics (DREADDs)Fear conditioning 15% · Self-administration 11% · Open field 11%
505 papers
OptogeneticsFear conditioning 25% · Operant conditioning 9% · Self-administration 8%
443 papers
In vivo electrophysiologyFear conditioning 12% · Novel object recognition 11% · Open field 10%
335 papers
Intranasal deliveryMorris water maze 22% · Forced swim test 15% · Novel object recognition 13%
299 papers
EEG and ECoGOpen field 19% · Morris water maze 18% · Novel object recognition 15%
265 papers
Intrathecal injectionVon Frey test 50% · Tail flick 12% · Hot plate 10%
257 papers
Fiber photometryElevated plus maze 13% · Operant conditioning 11% · Conditioned place preference 11%
177 papers
Transcranial stimulationMorris water maze 16% · Novel object recognition 16% · Open field 14%
152 papers
MicrodialysisSelf-administration 17% · Conditioned place preference 16% · Forced swim test 11%
133 papers
Deep brain stimulation (DBS)Open field 16% · Conditioned place preference 14% · Forced swim test 9%
92 papers
Calcium imaging in living animalsFear conditioning 15% · Novel object recognition 10% · Operant conditioning 10%
78 papers
Microglia depletionFear conditioning 22% · Open field 16% · Novel object recognition 14%
63 papers
Vagus nerve stimulation (VNS)Open field 25% · Forced swim test 17% · Elevated plus maze 17%
60 papers
Brain lesionsFear conditioning 20% · Morris water maze 15% · Elevated plus maze 13%
46 papers

The 5 brain techniques without a link have fewer than 100 such papers.

How we counted

A paper counts when it is animal research and its title or abstract mentions one of a brain technique's search terms and one of the 90 behavioral tests. A count is a number of papers, not of experiments, labs or animals. A paper that mentions the brain technique only in its methods is missed, so the counts run low.

Each technique has its own search terms, matched as whole words. Optogenetics counts only near a word such as light or stimulation, and fiber photometry only near a sensor or signal, such as GCaMP or calcium activity. A paper is animal research when its MeSH terms name an animal species. If they name none, NCBI's text-mining service PubTator 3 has to find one in the title or abstract. Papers on animals other than mice, rats, zebrafish, flies and primates count only when they also mention a test. Many of them study people or use the same words for something else. A paper can count under more than one brain technique.

The search terms for each brain technique are in the downloads below. The full method, with each source and its terms of use, is on our main page, Behavioral tests in published research.

Download the data

Each file covers both the animal models and the brain techniques.

How to cite

APA

ConductScience. (2026, November 13). Brain techniques in behavioral research [Data set]. https://conductscience.com/resources/data/brain-techniques

BibTeX

@misc{conductscience_brain_techniques_2026,
  author = {{ConductScience}},
  title = {Brain techniques in behavioral research},
  year = {2026},
  month = nov,
  url = {https://conductscience.com/resources/data/brain-techniques},
  note = {Data set, released November 13, 2026},
}

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