Endpoint methods library
Circadian & activity rhythm endpoint

Circadian spectral power

Strength of the 24-hour component in the activity spectrum, an objective measure of rhythm robustness from periodogram or cosinor analysis.

Unit
spectral power (arbitrary units)
Readout
Power (height) of the spectral peak at the circadian period
Assays
Home-cage video, wheel, beam-break, telemetry

Decision summary

Use circadian spectral power to compare rhythm strength objectively across animals or treatments. The activity time series is decomposed by FFT, Lomb-Scargle, or chi-square periodogram, and the power at the ~24 h period is read off as the rhythm strength. A tall 24 h peak means a strong clock; a flat spectrum means disruption.

Primary valuePower (height) of the spectral peak at the circadian period
Common unitsSpectral power, arbitrary units; or percent rhythm
Compatible assaysHome-cage video, wheel, beam-break, telemetry
Required boundarySpectral method, record length, and detrending
Do not infer alonePhase or the direction of any period change

Measurement notes

Bin the activity record, detrend it, and apply FFT, the Lomb-Scargle periodogram (preferred for gappy or unevenly sampled data), or the chi-square periodogram. Report the power at the dominant circadian peak. Cosinor fitting gives an equivalent parametric amplitude and goodness of fit.

Interpretation limit

Spectral power scales with overall activity and record length, so it is a relative measure best compared within a study under matched conditions. A reduced 24 h peak signals a weaker rhythm but does not say whether the clock is slow, fragmented, or mistimed.

Data capture

Store animal ID, spectral method and parameters, record length, detrending, peak period, peak power, and lighting condition.

Confound checks
  • Record length and sampling gaps changing the spectrum.
  • Overall activity level scaling power.
  • Detrending and windowing choices.
  • Ultradian components contaminating the estimate.
Reporting checklist
  • Spectral method (FFT, Lomb-Scargle, chi-square, cosinor) and parameters.
  • Record length, bin size, and detrending.
  • Peak period and peak power reported.
  • Lighting condition and activity measure.