Primary Assay — Water Flea
Acute Immobilization (OECD 202)
Daphnia magna
The 24-/48-h immobilization assay is the standard endpoint for chemical toxicity testing in aquatic ecotoxicology. Median effective concentration (EC₅₀) is reported.

Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Daphnia magna.
| Parameter | Unit | Description |
|---|---|---|
| EC₅₀ at 48h | mg/L | Median effect concentration |
| Immobilization rate | % | Fraction not swimming |
| Mortality | % | Lethal endpoint |
| No-Observed-Effect Concentration | mg/L | NOEC |
References
Citations for Acute Immobilization (OECD 202)
- OECD. (2004). Test Guideline 202: Daphnia sp. acute immobilisation test.
Compatible Equipment
Hardware for Water Flea Research
Glass Column Phototaxis Chamber
Vertical phototactic assay
Multi-Well Behavioral Imaging Plate
High-throughput toxicology
Heart-Rate Imaging Microscope
Cardiotoxicity
Predator-Cue Exposure Chamber
Inducible defense induction
Acute Immobilization Test Plate
OECD 202 endpoint
Related Assays
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Swimming Activity
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Run Acute Immobilization (OECD 202) on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your water flea facility.