Primary Assay — Drosophila
RING / Negative Geotaxis (Climbing Assay)
Drosophila melanogaster
Flies are tapped to the bottom of a vial and their climbing ability is recorded. The Rapid Iterative Negative Geotaxis (RING) assay enables high-throughput quantification. Sensitive to aging and neurodegeneration.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Drosophila melanogaster.
| Parameter | Unit | Description |
|---|---|---|
| Climb height at 4s | cm | Distance climbed in fixed time |
| % reaching top | % | Proportion clearing the vial |
| Climb speed | cm/s | Average upward velocity |
| Performance index | score | Composite of height and proportion |
References
Citations for RING / Negative Geotaxis (Climbing Assay)
- Gargano JW, Martin I, Bhandari P, Bhatt MS. (2005). Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila. Exp Gerontol, 40(5), 386-395. PMID: 15919590
- Madabattula ST, et al. (2015). Quantitative analysis of climbing defects in a Drosophila model of neurodegenerative disorders. J Vis Exp, (100), e52741. PMID: 26132365
Compatible Equipment
Hardware for Drosophila Research
Drosophila Activity Monitor (DAM)
Circadian activity and sleep
Climbing Assay Vials (RING)
Negative geotaxis
T-maze (Drosophila)
Olfactory learning and memory
Courtship Chambers
Mating behavior
Aggression Arenas
Fighting behavior
Fly Flight Tester
Flight ability screening
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Run RING / Negative Geotaxis (Climbing Assay) on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your drosophila facility.
