Primary Assay — Drosophila
Circadian Activity Monitoring (DAM System)
Drosophila melanogaster
Individual flies in glass tubes; infrared beam breaks record locomotor activity over days-weeks. Quantifies circadian rhythm, sleep, and activity patterns under light/dark or constant conditions.
Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Drosophila melanogaster.
| Parameter | Unit | Description |
|---|---|---|
| Activity counts | beam breaks/30min | Locomotor events per bin |
| Free-running period (τ) | hours | Endogenous rhythm period in constant conditions |
| Morning anticipation | index | Activity ramp before lights-on |
| Evening anticipation | index | Activity ramp before lights-off |
| Rhythm power (FFT) | relative | Strength of circadian rhythmicity |
References
Citations for Circadian Activity Monitoring (DAM System)
- Pfeiffenberger C, Lear BC, Keegan KP, Allada R. (2010). Locomotor activity level monitoring using the Drosophila Activity Monitoring (DAM) System. Cold Spring Harb Protoc, 2010(11), pdb.prot5518. PMID: 21041391
- Hendricks JC, et al. (2000). Rest in Drosophila is a sleep-like state. Neuron, 25(1), 129-138. PMID: 10707978
Compatible Equipment
Hardware for Drosophila Research
Drosophila Activity Monitor (DAM)
Circadian activity and sleep
Climbing Assay Vials (RING)
Negative geotaxis
T-maze (Drosophila)
Olfactory learning and memory
Courtship Chambers
Mating behavior
Aggression Arenas
Fighting behavior
Fly Flight Tester
Flight ability screening
Related Assays
Other Drosophila Primary Assays
04
Courtship Assay
Drosophila melanogaster
Drosophila courtship is a stereotyped behavioral sequence: orientation → following → wing extension (song) → licking → a…
04
RING / Negative Geotaxis (Climbing Assay)
Drosophila melanogaster
Flies are tapped to the bottom of a vial and their climbing ability is recorded. The Rapid Iterative Negative Geotaxis (…
04
Olfactory T-maze Learning
Drosophila melanogaster
Flies are trained to associate one odor with electric shock and tested for avoidance in a T-shaped choice point. Foundat…
Run Circadian Activity Monitoring (DAM System) on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your drosophila facility.
