ConductVision · Behavioral Analysis

Drosophila Olfactory Learning

Automated scoring of associative odor–shock conditioning and memory retention.

DrosophilaLearning & MemoryAuto Export
ConductVision / Drosophila Olfactory Learning
ChoiceCS+CS−Shock
Recording / Trial 3fly tracked
PI0.72
Learning Idx0.58
24h Retention0.35

Key Parameters

Metrics automatically extracted by ConductVision.

Performance Index

Proportion of flies choosing the non-conditioned odor minus those choosing the conditioned odor, normalized to total

Learning Index

Difference between trained and naive odor avoidance scores, isolating associative learning from innate odor bias

Memory Retention

Performance index measured at 1h (STM), 3h (MTM), and 24h (LTM) post-training to map memory phase kinetics

Avoidance Score

Per-fly displacement away from the conditioned odor arm, enabling individual-level analysis of learning phenotypes

Odor Discrimination

Choice accuracy between two olfactory stimuli at matched concentration, controlling for sensory deficits vs. learning deficits

Naive Odor Response

Baseline odor preference before conditioning, essential for separating sensory from cognitive phenotypes

+ 4 more parameters trackedShow all

Acquisition Rate

Performance index improvement across successive training trials, measuring the speed of odor–shock association formation

Extinction Rate

Decline in conditioned avoidance after repeated unreinforced odor exposures, indexing memory flexibility

Reversal Learning

Ability to reverse odor–shock contingency after CS+/CS− swap, a measure of cognitive flexibility and inhibitory control

Choice Latency

Time from T-maze release to arm entry, reflecting decision speed and motivational state during odor choice

What is Drosophila Olfactory Learning?

The Drosophila olfactory conditioning paradigm (T-maze or Y-maze odor choice) pairs an odor with electric shock. After training, flies avoid the shock-associated odor — a robust readout of associative learning and memory. Short-term, middle-term, and long-term memory can be assessed by varying the training-to-test interval.

ConductVision tracks fly distribution in choice apparatus arms, automatically computing performance and learning indices. The software supports high-throughput genetic screens of memory mutants and pharmacological studies of cognitive enhancers.

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