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Multi-Animal Tracking

Markerless identification of individual animals in group-housed paradigms

Track up to four animals simultaneously with persistent identity assignment — no ear tags, paint marks, or RFID implants.

Multi-Animal Tracking
98.7%
Identity accuracy
30fps
Acquisition frame rate
4
Simultaneous subjects
24
Supported paradigms
The problem

Physical markers introduce confounds and limit throughput

Group-housed behavioral experiments require individual identification. Current methods — paint marks, ear tags, RFID implants — each introduce their own confounds. Paint degrades within sessions, ear tags cause grooming artifacts, and implants require surgery. Manual scoring of social interactions is subjective and prohibitively slow for large cohorts.

  • Paint markers degrade during sessions, causing identity swaps and data loss in later time bins
  • RFID implants require invasive surgery, adding stress and post-operative recovery confounds
  • Manual scoring of social interactions is subjective, slow, and cannot capture all simultaneous events
The solution

Persistent identity from appearance alone

ConductVision assigns and maintains individual identities using appearance-based features extracted directly from video. Identity persists through occlusions, crossings, and huddling. All pairwise interactions are scored automatically and continuously.

  • Persistent identity assignment without any physical markers — purely vision-based
  • Identity maintained through occlusions, crossings, and close proximity at 98.7% accuracy
  • All pairwise interactions scored automatically — approach, contact, following, avoidance
Endpoints

Per-animal dependent variables in group paradigms

Individual trajectories per animal per frame

Individual trajectories per animal per frame

X/Y coordinates, velocity, and heading for each identified animal at every frame. Identity labels are consistent across the entire session.

CSVJSONMP4
Social proximity metrics

Social proximity metrics

Inter-animal distances, contact durations, approach/withdrawal counts, and following behavior scored for every dyad continuously.

CSV
Zone occupancy by individual

Zone occupancy by individual

Time in each defined zone reported per animal. Enables individual preference indices in social choice paradigms.

CSV
Applications

Group-housed paradigms requiring individual identification

Social preference

Three-chamber sociability

Track the test animal and stimulus animals independently. Compute social preference index, investigation time, and approach frequency without manual scoring.

Measures
  • Social preference index
  • Investigation duration
  • Chamber transition count
Aggression

Resident-intruder aggression

Identify resident and intruder throughout the session. Score attack latency, bout duration, and inter-animal distance without observer bias.

Measures
  • Attack latency
  • Aggression bout duration
  • Inter-animal distance
Social learning

Observer-demonstrator learning

Track observer and demonstrator separately to quantify observation time, proximity during demonstration, and subsequent behavioral mimicry.

Measures
  • Observation duration
  • Proximity to demonstrator
  • Behavioral mimicry index
Group phenotyping

Group open field phenotyping

Simultaneously phenotype multiple animals in the same arena. Individual locomotor profiles, thigmotaxis, and social clustering are computed in a single session.

Measures
  • Individual distance traveled
  • Thigmotaxis per animal
  • Social clustering index
Compared to typical systems

How ConductVision differs

FeatureConductVisionTypical systems
Subjects tracked simultaneously4 — markerless2 with physical markers
Temporal resolution30 fps per animal8-11 fps
Identity through occlusionYes — 98.7% accuracyFrequent identity swaps
Data granularityPer-animal per-frame coordinatesAggregate or manual annotation
Scoring methodFully automated, all dyadsManual or semi-automated

Try it with your own recordings

Upload a multi-animal recording and see individual trajectories extracted without markers.