NSF Certified Biological Safety Cabinet
NSF certified Class II Type A2 biosafety cabinet with HEPA filtration, 1210×600×660mm work area, and validated airflow containment for biological material handling.
The NSF Certified Class II Biosafety Cabinet Type A2 provides containment protection for personnel, product, and environment during work with biological materials. This recirculating cabinet maintains negative pressure within the work chamber while providing HEPA-filtered downflow air to protect samples from contamination. The Type A2 design exhausts 30% of air through a dedicated exhaust system while recirculating 70% through HEPA filtration, making it suitable for work with low-to-moderate risk biological agents and minute amounts of toxic chemicals.
With internal dimensions of 1210×600×660mm and a maximum sash opening of 470mm, this cabinet accommodates standard laboratory equipment and procedures. The airflow system maintains inflow velocities of 445-462 m³/h, downflow of 863 m³/h (60% recirculation), and exhaust of 462-587 m³/h (40% direct exhaust). NSF certification validates performance to established containment and airflow standards for biological safety cabinets.
Available Configurations
This biosafety cabinet is available in three configurations to meet different laboratory requirements:
- Class II A2 Standard — Full-size (1210×600×660mm work area), 70% recirculation / 30% exhaust. Ideal for general microbiology, cell culture, and pharmaceutical QC. $10,000
- Class II A2 – 2ft — Compact 2-foot work area with touchscreen controls and 99.9995% HEPA filtration. Space-efficient for smaller labs. $3,895
- Class II B2 (Total Exhaust) — 100% exhaust containment with dual HEPA filtration at 99.995% efficiency and motorized glass front. Maximum protection for volatile chemicals and radionuclides. $10,000
Use the "Cabinet Type" selector above to choose your configuration and see detailed specifications.
How It Works
The Class II Type A2 biosafety cabinet operates on the principle of directional airflow containment using HEPA filtration technology. Room air enters through the front opening and is immediately captured by negative pressure, preventing contaminated air from escaping the work chamber. This inflow air, along with recirculated air from the work surface, passes through a supply HEPA filter that removes 99.97% of particles 0.3 micrometers and larger.
The filtered air creates a protective downflow curtain over the work surface at approximately 863 m³/h, shielding samples from environmental contamination. Air is then drawn through perforated work surfaces into a rear plenum where it splits: 60% recirculates through the supply HEPA filter for reuse, while 40% is exhausted through a separate HEPA filter to the building exhaust system. This balanced airflow design maintains negative pressure containment while providing sterile working conditions.
Critical airflow velocities are maintained by calibrated fans and monitored through the control system. The cabinet maintains an average face velocity of 0.38-0.51 m/s at the sash opening, ensuring personnel protection without creating turbulent conditions that could compromise containment or sterile technique.
Features & Benefits
Size
- 3 ft
- 4 ft
- 6 ft
Weight
- 330.0 kg
Dimensions
- L: 222.0 mm
- W: 138.3 mm
- H: 77.5 mm
Comparison Guide
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Work Chamber Size | 1210×600×660mm internal dimensions | Entry-level models often provide 900×600×660mm or smaller work areas | Larger work surface accommodates multiple experiments and standard equipment configurations simultaneously |
| Airflow Design | Type A2 with 60% recirculation and 40% exhaust | Type A1 cabinets offer 70% recirculation with no exhaust capability | Exhaust pathway enables work with minute amounts of toxic chemicals while maintaining energy efficiency |
| Certification Standard | NSF certified performance validation | Some models lack third-party certification or use manufacturer testing only | Independent NSF certification provides verified containment performance for regulatory compliance |
| Sash Opening Height | 470mm maximum opening with 200mm tested safety height | Smaller units may have 400mm or less maximum opening | Greater access height facilitates equipment manipulation and reduces ergonomic strain during extended procedures |
| Downflow Velocity | 863 m³/h (507 CFM) downflow rate | Budget models often provide lower downflow rates around 600-700 m³/h | Higher downflow velocity provides more robust product protection against environmental contamination |
This NSF-certified biosafety cabinet combines substantial work area dimensions with validated Type A2 performance characteristics. The 40% exhaust capability and robust airflow rates provide versatility for biological and limited chemical applications while maintaining energy efficiency through partial air recirculation.
Practical Tips
Schedule annual NSF certification including face velocity, downflow uniformity, and smoke containment testing with qualified technicians.
Why: Maintains validated performance parameters and ensures continued regulatory compliance for biological containment.
Replace HEPA filters when pressure drop exceeds manufacturer specifications or during annual certification if performance declines.
Why: Degraded filters compromise both containment effectiveness and energy efficiency of the airflow system.
Allow cabinet to run for 5 minutes before use and avoid rapid arm movements that disrupt airflow patterns at the sash opening.
Why: Stabilized airflow and smooth technique maintain the protective air curtain essential for both personnel and product protection.
Position materials toward the rear of work surface and avoid placing items on airflow grilles or near the front sash opening.
Why: Proper item placement maintains unobstructed airflow patterns critical for containment and sterile working conditions.
If airflow alarms activate, check for blocked exhaust pathways, overloaded work surfaces, or damaged sash mechanisms before continuing work.
Why: Airflow disruptions compromise biological containment and must be resolved before resuming potentially hazardous procedures.
Never use the cabinet for volatile organic solvents or chemicals exceeding the minute quantity limitations of Type A2 design.
Why: Air recirculation can concentrate chemical vapors to unsafe levels since 60% of air is refiltered and returned to the work chamber.
Document airflow certification results and maintain logs of HEPA filter replacement dates for quality assurance compliance.
Why: Proper documentation demonstrates equipment validation history required for regulatory audits and research data integrity.
Clean work surfaces with appropriate disinfectants after each use and perform weekly decontamination of interior surfaces.
Why: Regular decontamination prevents cross-contamination between experiments and maintains sterile working environment.
Setup Guide
What’s in the Box
- NSF Certified Class II Type A2 Biosafety Cabinet (main unit)
- Power cord and electrical connections
- User manual and operation instructions
- NSF certification documentation
- Installation hardware (typical)
- Airflow verification smoke tubes (typical)
- Work surface cleaning protocol (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering defects in materials and workmanship, with technical support for installation, operation, and maintenance procedures.
Compliance
References
Background reading relevant to this product:
What types of biological agents can be safely handled in this Type A2 cabinet?
This NSF-certified Class II Type A2 cabinet is designed for BSL-2 biological agents including most bacteria, viruses, and cell cultures. With 40% exhaust capability, it can also handle minute amounts of toxic chemicals, but consult institutional biosafety committees for specific agent approvals.
How often does the cabinet require airflow certification and what parameters are tested?
Annual certification is typically required, testing face velocity (0.38-0.51 m/s), downflow velocity uniformity, containment (smoke patterns), and HEPA filter integrity. Some institutions require semi-annual testing for high-risk applications.
Can this cabinet be connected to building exhaust systems or does it require dedicated ducting?
The Type A2 design with 40% exhaust can operate with either canopy connections to building exhaust or direct hard ducting. Consult facility engineers for proper exhaust system integration and backdraft prevention.
What are the power requirements and installation considerations?
Consult product datasheet for specific electrical requirements. Installation requires level positioning, adequate clearance for service access, and proper exhaust connection. Cabinet weighs 330 kg requiring appropriate floor support.
How does air recirculation affect work with volatile chemicals?
The 60% air recirculation limits use to minute amounts of chemicals with low volatility and toxicity. For significant chemical work, consider Type B2 total exhaust cabinets or chemical fume hoods instead.
What maintenance procedures are required for continued NSF certification?
Routine maintenance includes HEPA filter replacement, airflow system cleaning, UV light replacement if equipped, and motor/blower servicing. Annual professional certification includes all critical airflow and containment testing.
Can standard laboratory equipment be operated inside the work chamber?
The 1210×600×660mm internal dimensions accommodate most standard equipment including microscopes, small centrifuges, and pipettors. Avoid blocking airflow grilles and maintain clear exhaust pathways for proper containment.
How does this cabinet compare to Class I or Class III biological safety cabinets?
Unlike Class I cabinets, this Class II provides both personnel and product protection through HEPA-filtered downflow. Compared to Class III glove boxes, it offers easier access and equipment use while maintaining appropriate containment for BSL-2 work.
Can this biological safety cabinet be used on a benchtop?
Yes. The BSC-3FA and BSC-4FA series cabinets are delivered as benchtop units with optional legs available separately. Before installation, ensure your workbench has sufficient load-bearing capacity: at least 200 kg for the BSC-3FA series and at least 250 kg for the BSC-4FA series.
What workbench load-bearing capacity is required for benchtop installation?
The minimum workbench load-bearing capacity required is 200 kg for the BSC-3FA series and 250 kg for the BSC-4FA series.
Have a question about this product?
Accessories
Enhance your setup with compatible accessories
