Primary Assay — Drosophila
Aggression Assay
Drosophila melanogaster
Male Drosophila fight over resources (food, territory, females). Stereotyped aggressive behaviors include lunging, wing threat, and chasing. Used to study monoamine pathways and social dominance.

Quantitative Output
Measured Parameters
Every parameter is automatically tracked frame-by-frame in the ConductVision pipeline for Drosophila melanogaster.
| Parameter | Unit | Description |
|---|---|---|
| Lunging frequency | events/10min | Primary aggressive contact |
| Wing threat displays | count | Bilateral wing elevation |
| Chase duration | s | Pursuit of subordinate |
| Dominance index | ratio | Win proportion over repeated bouts |
| Latency to first lunge | s | Aggression onset timing |
References
Citations for Aggression Assay
- Chen S, Lee AY, Bowens NM, Huber R, Bhatt DK. (2002). Fighting fruit flies: a model system for the study of aggression. Proc Natl Acad Sci U S A, 99(8), 5664-5668. PMID: 11960021
- Hoyer SC, et al. (2008). Octopamine in male aggression of Drosophila. Curr Biol, 18(3), 159-167. PMID: 18249112
Compatible Equipment
Hardware for Drosophila Research
Drosophila Activity Monitor (DAM)
Circadian activity and sleep
Climbing Assay Vials (RING)
Negative geotaxis
T-maze (Drosophila)
Olfactory learning and memory
Courtship Chambers
Mating behavior
Aggression Arenas
Fighting behavior
Fly Flight Tester
Flight ability screening
Related Assays
Other Drosophila Primary Assays

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Courtship Assay
Drosophila melanogaster
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RING / Negative Geotaxis (Climbing Assay)
Drosophila melanogaster
Flies are tapped to the bottom of a vial and their climbing ability is recorded. The Rapid Iterative Negative Geotaxis (…

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Circadian Activity Monitoring (DAM System)
Drosophila melanogaster
Individual flies in glass tubes; infrared beam breaks record locomotor activity over days-weeks. Quantifies circadian rh…
Run Aggression Assay on ConductVision
Our team will configure the protocol, camera rig, and analysis pipeline for your drosophila facility.