CO2 Shaking Incubator(customize)
Microprocessor-controlled CO2 incubator with orbital shaking capability, providing precise environmental control for suspension cell cultures with programmable temperature, CO2, and agitation parameters.
Louise Corscadden, PhD
Director of Science · ConductScience
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Key Specifications
Full details →- Incubator type
- CO2
- Temperature range
- 4 to 65 °C
- Max CO2 concentration
- 20%
- Max shaking speed
- 350 rpm
- Catalog number
- BIO-0231
The CO2 Shaking Incubator represents a specialized environmental control system designed for cell culture applications requiring precise atmospheric and agitation parameters. This microprocessor-controlled unit combines CO2 concentration regulation (0-20.0%) with orbital shaking capabilities (20-350 rpm) and temperature control (4-65°C) in a single platform. The forced convection design ensures uniform environmental conditions throughout the chamber, while the 7-inch touch screen interface provides programmable control over temperature, shaking speed, and timing parameters.
The system accommodates up to 48 standard 250ml culture vessels on its 485×485mm platform with a 26mm orbit diameter. The P.I.D microprocessor chip maintains temperature regulation within ±0.1°C and shaking speed precision within ±1 rpm. Multiple programmable modes enable researchers to establish complex culture protocols with automated timing up to 999.9 hours for extended experiments or continuous operation.
How It Works
The CO2 shaking incubator operates through integrated environmental control systems that maintain precise culture conditions. The CO2 regulation system monitors and adjusts atmospheric composition from 0-20.0% through automated gas injection and circulation. Temperature control utilizes a P.I.D microprocessor chip with forced convection to maintain uniform thermal conditions throughout the chamber, with feedback control achieving ±0.1°C precision.
The orbital shaking mechanism employs convolution motion with a 26mm orbit diameter to provide consistent agitation across the 485×485mm platform. The shaking system operates from 20-350 rpm with ±1 rpm precision, enabling researchers to optimize oxygen transfer and prevent cell aggregation. The platform can accommodate up to 48 standard 250ml culture vessels simultaneously.
The 7-inch touch screen interface enables programming of complex protocols through multiple programmable modes controlling temperature, shaking speed, and timing parameters. The system supports continuous operation or timed protocols up to 999.9 hours, with the capability for static culture when shaking is set to 0 rpm.
Features & Benefits
Research record
Use these values in methods sections and data records.
- Device type
- Incubator shaker
OBI:0001076 - Incubator type
- CO2
- Temperature range
- 4 to 65 °C
- Max CO2 concentration
- 20%
- Max shaking speed
- 350 rpm
This ID comes from the Ontology for Biomedical Investigations (OBI).
If this page lists several models or options, the temperature range, maximum CO2 concentration and maximum shaking speed may apply to only one of them.
Download the source data for this record as a JSON-LD file
Methods section wording
CO2 shaking incubator (ConductScience, Skokie, IL, USA; cat. no. BIO-0231)
Automation Level
- fully-automated
Control Model
- P.I.D microprocessor chip
Convection Model
- Forced convection
Shake Model
- Convolution
Control System
- Multiple Programmable mode(temp, speed, time)
Cyclotron Frequency Range(rpm)
- 0, 20~350(to do static culture)
Cyclotron Frequency Sensitivity(rpm)
- ±1
Orbit Diameter(mm)
- Φ26
CO2 Density Range
- 0~20.0%
Standard Capacity
- 250ml*48
Size of Platform(mm)
- 485*485
Timing Range(h)
- 0~999.9(Any time,continuous duty)
Temperature Range(℃）
- 4°C to 65°C(20°C)
Temperature Regulate Sensitivity(℃）
- ±0.1
Brand
- ConductScience
Research Domain
- Cancer Research
- Cell Biology
- Developmental Biology
- Immunology
- Microbiology
- Pharmaceutical QC
Display Type
- 7 Inch touch screen
Shipping weight
- 29.98 lb
Shipping box
- L: 42.0 cm
- W: 43.6 cm
- H: 38.0 cm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Temperature Range | 4-65°C with ±0.1°C precision | Many models offer 5-50°C with ±0.5°C precision | Extended temperature range enables both refrigerated and elevated temperature protocols with superior precision for temperature-sensitive cultures. |
| CO2 Control Range | 0-20.0% CO2 concentration | Standard models typically offer 0-10% CO2 range | Higher CO2 range accommodates specialized applications requiring elevated atmospheric conditions beyond standard cell culture requirements. |
| Shaking Speed Control | 20-350 rpm with ±1 rpm precision | Entry-level units often provide less precise speed control | High precision speed regulation ensures consistent agitation across the full operating range for reproducible culture conditions. |
| Platform Capacity | 485×485mm platform for 48×250ml vessels | Smaller units often accommodate 24-36 vessels | Higher capacity platform increases throughput for batch processing and high-volume culture applications. |
| Programming Interface | 7-inch touch screen with multiple programmable modes | Basic models use LED displays with limited programming | Advanced interface enables complex protocol programming and real-time parameter monitoring for sophisticated culture workflows. |
| Timing Control | 0-999.9 hours programmable timing | Standard models often limited to shorter timing ranges | Extended timing capability supports long-term culture experiments and continuous operation without manual intervention. |
This CO2 shaking incubator combines extended temperature range, precise environmental control, and high-capacity platform in a fully programmable system. The integration of CO2 regulation with orbital shaking eliminates the need for separate equipment while providing superior precision across all parameters.
Practical Tips
Verify CO2 concentration calibration monthly using certified gas standards and temperature calibration quarterly with NIST-traceable thermometers.
Why: Maintaining calibration accuracy ensures consistent culture conditions and reproducible experimental results.
Clean the chamber and shaking platform weekly with appropriate disinfectants and inspect the shaking mechanism for wear or imbalance.
Why: Regular maintenance prevents contamination and ensures smooth operation of the orbital shaking system.
Balance culture vessels symmetrically on the platform and secure all vessels with appropriate clamps to prevent movement during shaking.
Why: Proper loading prevents vibration and ensures uniform agitation across all culture vessels.
Allow the chamber to equilibrate for 30 minutes after parameter changes before introducing culture vessels.
Why: Equilibration time ensures stable environmental conditions and prevents temperature or CO2 fluctuations that could affect cell viability.
Monitor and record environmental parameters throughout critical culture periods using the touch screen interface or external logging systems.
Why: Parameter documentation provides traceability for experimental protocols and helps identify environmental factors affecting culture performance.
If shaking becomes irregular, check platform balance and vessel securing mechanisms before adjusting mechanical components.
Why: Imbalanced loads are the most common cause of shaking irregularities and can be corrected without mechanical adjustment.
Ensure CO2 supply lines are properly regulated and monitor for gas leaks using appropriate detection methods.
Why: Proper gas handling prevents over-pressurization and maintains safe CO2 levels in the laboratory environment.
Document all calibration activities and maintain certificates for temperature and CO2 reference standards used in verification procedures.
Why: Calibration documentation supports quality assurance requirements and regulatory compliance in controlled laboratory environments.
Setup Guide
What’s in the Box
- CO2 Shaking Incubator main unit
- Power cable (typical)
- CO2 gas connection tubing (typical)
- Platform securing hardware (typical)
- User manual and programming guide (typical)
- Calibration certificate (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering parts and labor, with technical support available for programming assistance and troubleshooting guidance.
Compliance
What is the temperature uniformity across the chamber during operation?
The forced convection system with P.I.D control maintains temperature regulation within ±0.1°C. Consult product datasheet for specific uniformity measurements across the chamber volume.
Can the shaking speed be programmed to change during a culture cycle?
Yes, the multiple programmable modes allow automated control of shaking speed along with temperature and timing parameters throughout the incubation protocol.
What types of culture vessels are compatible with the platform?
The 485×485mm platform is designed for up to 48 standard 250ml vessels. Larger flask sizes can be accommodated with reduced capacity depending on vessel dimensions.
How is CO2 concentration monitored and controlled?
The system includes integrated CO2 monitoring and injection controls. Consult product datasheet for sensor specifications and calibration requirements.
Is the unit suitable for anaerobic culture conditions?
The system controls CO2 from 0-20.0% but does not provide anaerobic gas mixing. For strict anaerobic conditions, a dedicated anaerobic workstation would be required.
What is the recovery time after door opening?
Recovery time depends on chamber size and ambient conditions. The forced convection system and P.I.D control optimize recovery, but specific times should be verified during installation.
Can protocols be saved and recalled for repeated experiments?
Yes, the touch screen interface with multiple programmable modes allows storage and recall of custom protocols combining temperature, CO2, speed, and timing parameters.
What maintenance is required for consistent performance?
Regular calibration verification of temperature and CO2 sensors is recommended, along with cleaning of the chamber and shaking mechanism according to the maintenance schedule in the user manual.
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