Multifunctional Fluorescence Imaging System

The W6000 Multifunctional Fluorescence Imaging System integrates four core imaging modules — fluorescence, chemiluminescence, tissue fluorescence, and gel imaging — into a single platform. Three top-mounted fluorescence excitation sources at 470 nm, 530 nm, and 650 nm enable simultaneous detection of multiple target proteins on a single membrane using fluorescently labeled secondary antibodies. A proprietary image inversion function allows standard pre-stained protein ladders to be used directly in fluorescent WB experiments: bright ladder bands captured in brightfield are fully resolved under darkfield after inversion processing, eliminating the need for fluorescence-conjugated ladder substitutes. A deep-cooled monochrome camera (−40°C relative to ambient) and motorized filter wheel serve both the fluorescence and chemiluminescence channels, while integrated 310/254/365 nm UV LED sources and motorized lens handle nucleic acid and protein gel documentation in the same instrument.

Key Features

• Three fluorescence excitation channels — 470 nm, 530 nm, 650 nm — top-mounted, symmetrically positioned
• Multiplex fluorescent WB: detect multiple target proteins simultaneously on a single membrane
• Image inversion function: use standard pre-stained protein ladders directly in fluorescent WB without fluorescence-conjugated ladder substitutes
• Deep-cooled 9 MP monochrome camera: −40°C relative to ambient; dark current 0.0003 e⁻/s/pixel at −15°C
• Motorized filter wheel switches between fluorescence, chemiluminescence, and gel imaging modes
• Chemiluminescence WB: 0.1 s fine-increment exposure adjustment; time accumulation; one-click ladder removal
• Tissue fluorescence imaging: autofluorescence detection with real-time frame selection for quantitative analysis
• Cell and microbial fluorescence: rapid assessment in culture plates and bacterial agar plates
• Nucleic acid gel imaging (310 nm transmitted + 254/365 nm side UV); compatible with EB, SYBR Green, SerRed, SerBlue, GoldView
• Gel band excision with UV laser safety shield (OD > 7); protein gel imaging in color and grayscale

Applications

• Multiplex fluorescent Western blot imaging
• ECL chemiluminescence Western blot detection
• Tissue section autofluorescence imaging
• Fluorescent protein expression imaging in cell culture plates
• Microbial fluorescence assays on bacterial agar plates
• Nucleic acid gel imaging (EtBr, SYBR Green, SerRed, SerBlue, GoldView)
• Protein gel imaging (Coomassie blue, silver stain)
• Nucleic acid gel band excision and downstream purification

Technical Specifications

Ordering Information

Also Available

For chemiluminescence-only Western blot imaging, see the W2000 Plus. For combined chemiluminescence WB and gel documentation without fluorescence channels, see the W5000 Plus. For dual-camera ECL imaging with true-color pre-stained marker capture, explore the W3000 Plus.