Multiplex Fluorescence Imaging System W3000 Plus
Dual-camera chemiluminescence imaging system for Western blot detection. Dedicated deep-cooled 9 MP monochrome camera for high-sensitivity ECL band detection plus an independent 45 MP color camera for true-color pre-stained ladder imaging. One-click marker restoration eliminates non-specific antibody cross-reactivity. 136×136 mm field of view (expandable to 200×200 mm).
Louise Corscadden, PhD
Director of Science · ConductScience
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The W3000 Plus Chemiluminescence Imaging System combines two independent optical channels: a deep-cooled monochrome camera for ECL Western blot band detection and a 45 MP color camera for true-color pre-stained ladder imaging. The monochrome channel records the chemiluminescent signal with 16-bit fidelity and full-range sensitivity, while the color channel captures the marker in natural color — eliminating the color-shift artifacts and non-specific cross-reactivity that occur in single-camera systems where the same sensor handles both signals. A one-click marker restoration function removes non-specific antibody binding signals from the marker and restores the true band pattern. The complete time-lapse exposure record allows any frame to be selected in 0.1 s increments after the experiment is complete, and underexposed samples can receive additional exposure without discarding the initial acquisition.
Key Features
- Dual-camera design: independent monochrome ECL channel + color marker channel
- Monochrome camera: 9 MP, deep-cooled −40°C relative to ambient; dark current 0.0003 e⁻/s/pixel at −15°C
- Color camera: 45 MP, 2.315×2.315 µm pixels, 1.4" sensor (18.93×13 mm)
- One-click marker restoration: removes non-specific antibody signals; recovers true marker band colors
- 16-bit time-lapse acquisition for monochrome channel; any frame selectable in 0.1 s increments
- Time accumulation: extend exposure on top of the original run without data loss
- Three exposure modes: High Quality / Standard / High Sensitivity
- Simultaneous imaging of 10 or more membranes
- 136×136 mm working area (expandable to 200×200 mm)
- 10.4-inch Windows 10 display; 16 GB RAM, 512 GB SSD, Wi-Fi/Bluetooth; USB 3.0 × 2
Applications
- ECL chemiluminescence Western blot detection with co-registered color ladder
- True-color pre-stained protein ladder documentation
- High-throughput multi-membrane WB workflows
- Experiments requiring accurate molecular weight co-localization
- Quantitative band density analysis
Technical Specifications
| Parameter | Camera 1 (Monochrome — ECL) | Camera 2 (Color — Marker) |
|---|---|---|
| Dimensions (W×D×H) | 400×371×700 mm | |
| Camera Type | Black and White Camera | Color Camera |
| Pixel Resolution | 9 million pixels | 45 million pixels |
| Resolution | 2992×3000 | 2992×3000 (as stated; see note) |
| Pixel Size | 3.76×3.76 µm | 2.315×2.315 µm |
| Target Size | 1" (11.28×11.28 mm) | 1.4" (18.93×13 mm) |
| Full Well Capacity | 16.5 ke⁻ (HCG), 50.5 ke⁻ (LCG) | 10.8 ke⁻ |
| Sensitivity | 877 mV @ 1/30 s | 419 mV |
| Readout Noise | 1.24 e⁻ (HCG), 3.22 e⁻ (LCG) | 2.12 e⁻ |
| Dark Current | 0.0003 e⁻/s/pixel at −15°C | 0.12 mV |
| Signal-to-Noise Ratio | 42.2 dB (HCG), 47 dB (LCG) | 40.3 dB |
| Exposure Time | 0.1 ms – 1 h | 17 µs – 15 s |
| Binning Mode | 1×1, 2×2, 3×3 | 1×1, 2×2, 3×3 |
| Grayscale | 16-bit (65,536 levels) | 8-bit (256 gray levels) |
| Cooling | Relative to ambient temperature −40°C | — |
Note: Camera 2 resolution is listed as "2992×3000" on the source specification page.
Based on sensor dimensions (18.93×13 mm) and pixel pitch (2.315 µm), the physical
pixel count is approximately 45 MP. These two values may reflect different reporting
conventions. Confirm with the product datasheet for your application.
| Parameter | Specification |
|---|---|
| Lens (Camera 1) | |
| Type | Prime lens |
| Focal Length | 17 mm |
| Aperture | F0.95–F16 |
| Light Sources | |
| Bright-Field | Downward-facing LED white light, symmetrically distributed on both sides |
| Dark Box | |
| Light Isolation | Fully light-sealed; isolates environmental light |
| Door Control | Door sensor controls bright-field LED on/off |
| Imaging Area | |
| Effective Field of View | 136×136 mm (expandable to 200×200 mm) |
| Software | |
| Exposure Modes | High Quality / Standard / High Sensitivity |
| Auto Exposure | Intelligent exposure with automatic binning |
| Real-time Imaging | Yes — live signal preview with overexposure indication |
| Time-lapse Imaging | Yes — retrieve any frame within the recorded exposure window |
| Time Accumulation | Yes — extend exposure after initial run |
| Optional Settings | Color Marker / Black & White Marker; Overexposure Prompt / No Overexposure Prompt |
| System | |
| Industrial Computer | 10.4-inch display (1024×768), Windows 10, 16 GB RAM, 512 GB SSD, Bluetooth/Wi-Fi |
| External Interfaces | USB 3.0 × 2 |
| Operating Voltage | 90–132 VAC / 180–264 VAC (selectable via switch), 47–63 Hz |
| Power Consumption | ≤200 W |
| Net Weight | 23.45 kg |
Ordering Information
| Cat. No. | Description |
|---|---|
| CS-W3000 Plus | Multiplex Fluorescence Imaging System W3000 Plus — Set |
Also Available
Need a simpler chemiluminescence-only system? See the W2000 Plus. For combined WB and gel imaging in one instrument, explore the W5000 Plus. For advanced fluorescence Western blot imaging (up to 6-plex), see the W6000.
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