Multifunctional Imaging System W5000 Plus
Multifunctional imaging system combining ECL chemiluminescence Western blot detection, nucleic acid gel imaging, gel band excision, and protein gel imaging. Deep-cooled 9 MP monochrome camera; 310/254/365 nm LED UV; rotating filter disc for mode switching; WB field of view 155×155 mm, nucleic acid gel 215×215 mm; supports simultaneous imaging of 10 or more membranes.
Louise Corscadden, PhD
Director of Science · ConductScience
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The W5000 Plus Multifunctional Imaging System integrates ECL chemiluminescence Western blot detection and nucleic acid/protein gel documentation into a single platform. A deep-cooled 9 MP monochrome camera provides the sensitivity required for ECL signal capture, while an integrated rotating filter disc and three-wavelength LED UV system switch the same optical path to gel imaging mode without reconfiguration. Standard 310 nm transmitted, 254 nm, and 365 nm UV sources cover EtBr, SYBR Green, SerRed, SerBlue, GoldView, and other common nucleic acid stains. A built-in UV laser protection shield (OD > 7) enables safe gel band excision inside the instrument. The time-lapse acquisition engine records the full chemiluminescence signal timeline so strong and weak bands on the same membrane can both be retrieved from a single exposure run. Up to 10 membranes can be imaged simultaneously.
Key Features
- Deep-cooled monochrome camera: −40°C relative to ambient; dark current 0.0003 e⁻/s/pixel at −15°C
- 9 MP sensor (2992×3000, 3.76×3.76 µm); 16-bit (65,536 levels); SNR 42.2 dB / 47 dB (HCG/LCG)
- Motorized zoom lens (17 mm, F0.95–F16) with rotating filter disc for mode switching
- Three UV wavelengths: 310 nm transmitted LED array + 254 nm / 365 nm side-mounted LED
- WB membrane field of view: 155×155 mm; protein gel: 155×155 mm; nucleic acid gel: 215×215 mm
- Simultaneous imaging of up to 10 membranes on a single acquisition run
- Gel cutting mode: extractable UV source with UV protective shield (OD > 7)
- Time-lapse imaging and time accumulation — retrieve any frame within the recorded exposure window
- Color marker imaging with marker optimization function for overexposed or cross-reactive markers
- 10.4-inch Windows 10 display; 16 GB RAM, 512 GB SSD, Wi-Fi/Bluetooth; USB 3.0 × 2
Applications
- ECL chemiluminescence Western blot detection
- Nucleic acid gel imaging (EtBr, SYBR Green, SerRed, SerBlue, GoldView)
- Protein gel imaging (Coomassie blue, silver stain, SYPRO Ruby)
- Nucleic acid gel band excision and downstream purification
- High-throughput multi-membrane WB workflows
- Combined WB and gel documentation without instrument switching
Technical Specifications
| Parameter | Specification |
|---|---|
| Dimensions (W×D×H) | 400×371×700 mm |
| Camera | |
| Camera Type | Black and White (Monochrome) Camera |
| Pixel Resolution | 9 Megapixels |
| Resolution | 2992×3000 |
| Pixel Size | 3.76×3.76 µm |
| Target Size | 1" (11.28×11.28 mm) |
| Full Well Capacity | 16.5 ke⁻ (HCG), 50.5 ke⁻ (LCG) |
| Sensitivity | 877 mV @ 1/30 s |
| Readout Noise | 1.24 e⁻ (HCG), 3.22 e⁻ (LCG) |
| Dark Current | 0.0003 e⁻/s/pixel at −15°C |
| Signal-to-Noise Ratio | 42.2 dB (HCG), 47 dB (LCG) |
| Exposure Time | 0.1 ms – 1 h |
| Binning Mode | 1×1, 2×2, 3×3 |
| Grayscale | 16-bit (65,536 gray levels) |
| Cooling | Relative to ambient temperature −40°C |
| Lens | |
| Type | Motorized zoom lens |
| Focal Length | 17 mm |
| Aperture | F0.95–F16 |
| Light Sources | |
| Bright-Field | Downward-facing LED white light, symmetrically distributed on both sides |
| Transmitted UV | 310 nm LED array with uniform transmitted illumination |
| Side UV | 254 nm / 365 nm LED, symmetrically distributed on both sides |
| Dark Box | |
| Light Isolation | Fully light-sealed; isolates environmental light |
| Door Control | Door sensor controls bright-field LED on/off |
| Rotating Disc | Filter switching between chemiluminescence and gel imaging modes |
| Gel Cutting | Extractable UV source + UV protective board (OD > 7) |
| Imaging Area | |
| WB Membrane / Protein Gel | 155×155 mm effective field of view |
| Nucleic Acid Gel | 215×215 mm effective field of view |
| Software | |
| Exposure Modes | High Quality / Standard / High Sensitivity |
| Auto Exposure | Intelligent exposure with automatic binning; one-operation optimal imaging |
| Real-time Imaging | Yes — live signal preview with overexposure indication |
| Time-lapse Imaging | Yes — retrieve any frame within the recorded exposure window |
| Time Accumulation | Yes — extend exposure after initial run |
| System | |
| Industrial Computer | 10.4-inch display (1024×768), Windows 10, 16 GB RAM, 512 GB SSD, Bluetooth/Wi-Fi |
| External Interfaces | USB 3.0 × 2 |
| Operating Voltage | 90–132 VAC / 180–264 VAC (selectable via switch), 47–63 Hz |
| Power Consumption | ≤300 W |
| Net Weight | 30.65 kg |
Ordering Information
| Cat. No. | Description |
|---|---|
| CS-W5000 Plus | Multifunctional Imaging System W5000 Plus — Set |
Also Available
Need chemiluminescence-only imaging with a minimal footprint? See the W2000 Plus. For dual-camera marker imaging with one-click marker restoration, explore the W3000 Plus. For gel imaging only (no WB), see the W1000 Plus. For advanced fluorescence Western blot imaging, see the W6000.
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