Description
The cannula implantation system is an accepted method of acute and chronic intracranial drug administration for animal experiments.A single cannula system includes a guide cannula, dummy cannula, internal cannula, and fixing screw. Together with our stereotaxic instruments, holders, fixing screws, PE cannulas, injection pumps, and other related products, this system provide a set of simple and effective experimental tool for unilateral intracranial administration for neuroscientists. More importantly, we offer users can customized cannulas based on their specific experimental requirements.
Cannula Models and Specifications
Guide Cannula [OD x ID]* | Internal Cannula [OD x ID]** | Dummy Cannula Plastic [OD]*** | Dummy Cannula Metal [OD]*** |
---|---|---|---|
RWD-62001 (23g) (0.64 x 0.45) | RWD-62201 (0.41×0.25) | RWD-62101 0.4 | RWD-62106 0.4 |
RWD- 62002 (24G) (0.56 × 0.38) | RWD-62202 (0.36×0.2) | RWD-62102 0.3 | RWD-62107 0.3 |
RWD-62003 (26G) (0.48×0.34) | RWD-62203 (0.3×0.14) | RWD-62102 0.3 | RWD-62107 0.3 |
RWD-62004 (27G) (0.41×0.25) | RWD-62204 (0.21×0.11) | RWD-62104 0.2 | RWD-62108 0.2 |
RWD-62005 (22G) (0.71×0.45) | RWD-62201 (0.41×0.25) | RWD-62101 0.4 | RWD-62106 0.4 |
Note: Unit of data in the table is mm. Uniform height of the single cannula base: 6mm. Width of the base: 3.5mm. Dummy cannula is available in plastic or metal.
*C value: length of the stainless steel tube of the Guide cannula 0.5 ~ 15mm
**G1 value:the length of the protruding part of the internal cannula. 0, 0.5 ~ 15mm
***G2 value: The length of the protruding part of the dummy cannula. Plastic or metal option. 0, 0.5 ~ 15mm
Accesories
Product Type | Product Description |
---|---|
Fixing Screw | RWD-62502 |
PU Tube | RWD-BC-22 |
Plug | RWD-P22-R/ RWD P22-W |
Cannula Holder | RWD-68205/ RWD 68217 |
C*:Length Customization Range (mm) | 0.5~15 mm |
G1*:Length Customization Range (mm) | 0, 0.5~15 mm |
G2*:Length Customization Range (mm) | 0, 0.5~15 mm |
C* value: length of the stainless steel tube of the Guide cannula. C = depth of target brain region + thickness of dental cement (0.5 ~ 0.8 mm) – G1.
G1* value: the length of the protruding part of the internal cannula when it is inserted into the guide cannula, can be customized. Usually, 0.5 mm or 1.0 mm is selected, and 0.5 mm is recommended.
G2* value: The length of the protruding part of the dummy cannula when it is inserted into the guide cannula, can be customized. Usually 0, 0.5mm or 1.0 mm is selected, 0 mm is recommended.
Cannula components
Cannula component | Product Description |
---|---|
Guide cannula | Guide cannula consists of a cylindrical base with threads and a thin stainless steel tube. It is pre-implanted intracranially and secured with dental cement around the injection orifice. Both dummy cannula and internal cannula can be inserted into the guide cannula individually for sealing or injections. |
Internal cannula | Internal cannula connects with the guide cannula on one end and with PU tube on the other end for injections. |
Fixing screw | Fixing screw is used to fix the relative position of the internal cannula and the guide cannula to ensure stable connection and avoid leakage. |
Dummy cannula | Dummy cannula is composed of a stainless steel wire and a threaded plastic cap. For use, it should be inserted in and fixed on the guide cannula to prevent tissue fluid from entering the catheter and causing it to become blocked. The length of the stainless steel wire can be customized or cut according to actual needs.Optional plastic or metal material (more durable). |
PU tube | PU tube connects with the internal cannula on one end and syringe on the other end for drug injection. |
Plug | Plug connects with PU tube on the one end and with syringes of different sizes on the other end. |
Screw | Screw provides the dental cement with a point of strength to prevent it from falling off. |
Cannula selection: Mice and Rat options
Step 1. Selecting the guide cannula size according to the experimental animal and the depth of the target brain region.
Guide cannula size selection for rat: 62005 (22G), 62001 (23G), 62002 (24G).
Guide cannula size selection for mouse: 62003 (26G), 62004 (27G).
Product Type | Product Description |
---|---|
Guide cannula size selection for Rat | RWD-62005 (22G) RWD-62001 (23G) RWD-62002 (24G) |
Guide cannula size selection for Mouse | RWD-62003 (26G) RWD-62004 (27G) |
Step 2. Determine the C value: length of the stainless steel tube of the Guide cannula. C = depth of target brain region + thickness of dental cement (0.5 ~ 0.8 mm) – G1.
Step 3:
Determine the G1 value:the length of the protruding part of the internal cannula when it is inserted into the guide cannula.Usually 0.5 mm or 1.0 mm is selected, and 0.5 mm is recommended.
Step 4:
Determine the G2 value: The length of the protruding part of the dummy cannula when it is inserted into the guide cannula.Usually 0, 0.5mm or 1.0 mm is selected, 0 mm is recommended.
Step 5:
Selecting the screws (recommended diameter slightly larger than the diameter of the skull drill), plugs, catheters, and other accessories.
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