Behavioral Mazes

Pole Test

SKU 5019
$690.00
Unit: Each

Vertical pole apparatus for assessing motor coordination and movement disorders in rodent models of Parkinson’s disease and dopaminergic dysfunction.

CE MarkedBAA available
Species SKU 5019
$690.00
Unit: Each
Scientist guidance
Louise Corscadden, PhD, Director of Science

Louise Corscadden, PhD

Director of Science · ConductScience

Ask Louise about Pole Test fit, setup, configuration, or quote prep.

Key Specifications

Full details →
Model fit
Mouse, Rat
SKU family
CS-958382
Sizing
Mouse Pole test: Diameter 8 mm · Length 50 cm; Rat Pole test: Diameter 10 mm · Length 63 cm
Ordering
Online checkout and quote request available
Category
Behavioral Mazes
Build notes
Confirm accessories, station layout, and support needs before purchase
Category: Behavioral Mazes

The Pole Test is a standardized behavioral apparatus designed to assess motor coordination and movement disorders in rodents. This simple yet sensitive test evaluates an animal's ability to orient and descend from a vertical pole, providing quantitative measures of motor dysfunction commonly associated with nigrostriatal damage and dopaminergic system impairment.

The apparatus consists of a vertical pole with species-specific diameters (8 mm for mice, 10 mm for rats) that allows researchers to measure turning time, total descent time, and movement quality. The test is particularly valuable for evaluating motor phenotypes in Parkinson's disease models, including 6-OHDA lesioned animals and MPTP-treated subjects, where bradykinesia and postural instability are primary endpoints.

*TUB NOT INCLUDED

How It Works

The pole test exploits the natural tendency of rodents to turn head-down and descend when placed on a vertical pole. Normal animals rapidly orient themselves and descend smoothly, while animals with motor dysfunction exhibit prolonged turning times and hesitant, bradykinetic movements during descent.

The test measures two primary parameters: turning time (latency to orient head-down) and total descent time (time to reach the base). Additional qualitative assessments include movement fluidity, number of slips, and use of alternative descent strategies. These measures correlate with the degree of striatal dopamine depletion and provide a sensitive index of motor impairment.

The apparatus diameter is optimized for each species to ensure appropriate grip difficulty - too narrow causes slipping in healthy animals, while too wide fails to challenge motor-impaired subjects. The standardized dimensions (8 mm for mice, 10 mm for rats) have been validated across multiple Parkinson's disease models.

Features & Benefits

Species-specific pole diameters
Optimized grip challenge for mice (8 mm) and rats (10 mm) ensures appropriate difficulty level for motor assessment
Standardized vertical design
Consistent test conditions enable reliable comparison across studies and laboratories
Simple apparatus construction
Minimal technical requirements allow focus on behavioral endpoints rather than equipment complexity
Quantitative timing measures
Objective assessment of turning time and descent time provides statistical power for group comparisons
Compatible with video analysis
Supports automated scoring and detailed movement analysis for comprehensive motor evaluation
Storage container included
Organized storage prevents component loss and maintains apparatus condition between experiments
Assembly required design
Compact shipping and secure setup ensure apparatus stability during testing

Track rodents with ConductVision

ConductVision is our video-tracking software. Record your sessions on video, and it scores standard rodent tests, such as those listed below.

Examples of tests it scores

To check whether ConductVision scores your protocol in this apparatus, ask in your quote request.

Add ConductVision to your quote

ConductVision

$1,490 per year

A 5-year license is $3,990 in total.

Results export as CSV files.

Ask a question

Have a question? Just ask.

Send it over and we'll email you a personalized answer, with no call or scheduling.

Personalized email reply
Usually within one business day
Helpful answers published here

Prefer to talk it through?

Ask your question

Type it below and we'll email you a personalized answer, with no meeting required.

We'll only use your email to send your answer; broadly useful answers may be published anonymously.

Accessories

Enhance your setup with compatible accessories

Total: $0.00

Frequently Bought Together

Total: $2,730.00

Creator Insights

Norio OgawaIntroduced the pole test of bradykinesia in MPTP mice (1985)Okayama University Medical School, Japan

About the Creator

Norio Ogawa led research on experimental models of Parkinson’s disease in the Department of Neuroscience at Okayama University Medical School. Working shortly after the 1983 discovery that MPTP selectively destroys nigrostriatal dopamine neurons, Ogawa and colleagues developed a reproducible MPTP mouse model and, in 1985, introduced the pole test: a mouse placed head-upward atop a vertical pole is timed as it turns downward and descends, giving a quantitative index of bradykinesia. Their paper established the test’s sensitivity to dopamine-restoring interventions and validated it as a quantitative motor readout in Parkinsonian models. The pole test is now one of the most widely used motor endpoints in rodent Parkinson’s research, often run alongside the rotarod and cylinder tests.

To view Norio Ogawa’s publications, visit PubMed.

Are you Norio Ogawa? to review your photo and bio, and find out how to submit Creator Insights.

ConductScience celebrates method creators: researchers who, through rigorous and often ingenious experiments, develop the tools that reveal how the brain and body work. These are real scientific discoveries that become everyday instruments for the labs that follow.

Foundational paper
  1. Ogawa, N., Hirose, Y., Ohara, S., Ono, T., & Watanabe, Y. (1985). A simple quantitative bradykinesia test in MPTP-treated mice. Research Communications in Chemical Pathology and Pharmacology, 50(3), 435–441. Link

§ 1

Introduction

The Pole Test measures bradykinesia and motor initiation by recording how long a rodent takes to turn head-down at the top of a vertical pole and to descend to the base. Ogawa and colleagues introduced the test as a simple quantitative measure of bradykinesia in dopamine-depleted mice. 1

The two core readouts are time to turn, an index of movement initiation, and time to descend, which captures the full turning-and-climbing sequence. Because turning head-down on a narrow pole requires coordinated initiation and postural adjustment, the test is widely used to characterize sensorimotor anomalies in models of striatal dopamine depletion. 1

Pole texture and diameter, training state, body weight, motivation to descend to the home cage, and motor learning all change turn and descent times independent of true motor initiation. A defensible protocol fixes pole geometry and surface, trains animals to a stable head-up start, scores falls and missteps separately, and reports turn-direction bias as a quality-control flag. 1

§ 2

Methods

2.1 Procedure

Head-up start with time-to-turn and time-to-descend scoring, fall and misstep classification, and turn-direction tracking.

Pre-test setup

  1. 1.Acclimation and habituation: Habituate animals to the room and to the home cage at the base of the pole so the first measured trial reflects motor initiation rather than novelty or handling stress.
  2. 2.Apparatus calibration: Verify pole diameter, height, and surface texture, place the home cage and padding at the base, and confirm the camera captures the turn and full descent for frame-by-frame scoring.
  3. 3.Training to baseline: Train animals over consecutive trials to start head-up at the top and turn reliably, so test-day data reflect motor initiation rather than learning the task.
  4. 4.Define scoring rules: Pre-define time to turn, time to descend, what counts as a fall, what counts as a misstep, and how trials are capped for animals that slide rather than climb.

Trial sequence

  1. 1.Place head-up at the top: Position the animal head-up near the top of the pole and release it, starting the timer at release.
  2. 2.Record time to turn: Record the time from release until the animal completes a head-down turn, the index of movement initiation.2
  3. 3.Record time to descend: Record the total time from release until all four paws reach the base, capping at the pre-defined maximum for animals that stall or slide.3
  4. 4.Classify falls and missteps: Record falls and missteps as distinct outcomes rather than folding them into descent time, since each reflects a different coordination or grip failure.
  5. 5.Log turn bias and repeat: Note turn direction to flag a directional bias, then clean the pole between subjects to remove odor and urine cues before the next trial.

Critical methodological constraints

  • Pole texture and diameter. A smooth pole causes sliding that confounds descent time; geometry and surface must be held constant.2
  • Training state. Train to a stable head-up start and reliable turn before the test session.3
  • Body weight. Heavier animals descend differently independent of motor initiation. Report body weight and consider it when groups differ in mass.

2.2 Measurement & Analysis

Core pole-test endpoints for bradykinesia, motor initiation, and quality control.

Time To Turn (T-turn)

Bradykinesia

Time from release until the animal completes a head-down turn at the top of the pole, the primary index of movement initiation.2

Time To Descend (T-total)

Motor initiation & descent

Total time from release until all four paws reach the base, capturing the full turning-and-climbing sequence.3

Falls/Slips

Coordination failure

Number of falls or slips down the pole, recorded as a distinct outcome rather than folded into descent time.

Missteps

Grip control

Count of paw missteps or slips during the controlled descent, indexing grip and limb-placement control.

Turn Direction Bias

Quality-control flag

Consistent preference for turning one direction, which can flag unilateral motor asymmetry or an apparatus artifact.

+ Additional metrics: trial number, pole surface, body weight, latency to start moving, descent strategy (climb versus slide), and per-trial video notes.

2.3 descent-time fraction (analysis)

A compact fraction of the maximum trial window taken up by turning and descending.

Inline calculator

Type the values your tracker recorded.

Full calculator with 95% CI ->
Descent-time fraction

30.0%

Formula: turn + descent time / (turn + descent time + max-trial remaining) x 100. Interpret with pole texture, training state, body weight, and fall or misstep counts because a long descent can reflect sliding or grip failure rather than bradykinesia. 1

2.4 sample-size planning

Estimate the N per group needed to detect a literature-anchored motor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.

Striatal dopamine-depleted vs control mouse on a textured pole; representative magnitudes from Matsuura et al. (1997) pole-test validation.2

Cohen's d

2.14

N per group at 80% power

4

Total N

8

With attrition cushion

9

At 70% / 90% power

3 / 5

Methods sentence

Need ANOVA, proportions, paired design, or a power curve? Open in the full Sample-Size Calculator →

Formula: n = 2 · ((zα/2 + zβ) / d)2, where d = |μ₁ − μ₂| / σ. Assumes equal allocation, normality, and homoskedasticity. The attrition cushion inflates total N by 1 / (1 − dropout); confirm with your IACUC.

§ 3

Results

3.1 Sample apparatus output

Representative output from a textured-pole session with a head-up start and a 30 s trial cap.

Table 1 · Per-animal Pole Test scoring output

AnimalGroupTime to turnTime to descendFallsDescent-time fraction
PT-001Control2.0 s8.2 s027.3%
PT-002Control1.9 s7.6 s025.3%
PT-003Control2.4 s9.1 s030.3%
PT-004Parkinsonian6.5 s17.4 s158.0%
PT-005Parkinsonian7.2 s19.0 s263.3%
PT-006Parkinsonian6.8 s18.2 s260.7%

Synthetic example for illustration only. Score falls and missteps distinctly and check pole surface before interpreting descent times as bradykinesia.

§ 4

Discussion

Limitations of the paradigm, methodological caveats, and current directions.

4.1 Common confounds

Variables that can shift Pole Test results apart from the effect under study.

Pole texture/diameter

Surface texture and diameter set grip and descent difficulty. A smooth pole causes sliding that confounds descent time, so geometry and surface must be held constant.

Training state

Untrained animals confound motor initiation with task acquisition. Train to a stable head-up start and reliable turn before testing.

Body weight

Heavier animals descend differently independent of motor initiation, so weight should be reported and considered when groups differ in mass.

Motivation (home-cage at base)

A home cage or familiar enclosure at the base motivates descent. A weak incentive increases stalling and inflates descent time without a true deficit.

Motor learning

Turn and descent times improve across early trials as animals learn the task, so a single session can confound bradykinesia with task acquisition.

Confound checklist

Tick the confounds your protocol addresses, then export a methods-paragraph blurb you can paste into your manuscript.

Preview exported markdown

4.2 Construct validity caveats

The pole test is strongest when pole surface and geometry, training, and scoring rules are fixed before testing, and falls and missteps are recorded separately from descent time. Time to turn and time to descend index different stages; report them separately and confirm bradykinesia with an independent motor assay such as gait analysis or the rotarod. 1

4.3 Special considerations

When should I use the rotarod instead?

Use the rotarod for gross coordination and endurance under forced locomotion. The pole test is more specific to movement initiation and bradykinesia, especially in models of striatal dopamine depletion.

Why separate time to turn from time to descend?

Time to turn indexes movement initiation while time to descend captures the full turning-and-climbing sequence. Reporting them separately distinguishes an initiation deficit from a descent or grip problem.

How do I prevent sliding from confounding descent time?

Use a textured or gauze-wrapped pole so animals climb rather than slide. A smooth surface lets animals slide down, which shortens descent time without reflecting coordinated motor control.

4.4 Current directions

Quarterly editorial review of emerging Pole Test methodology. Q2 2026

Methods

Standardized pole surface

Specifying pole texture and diameter improves comparability of turn and descent times between labs and reduces sliding artifacts.

Emerging

Video-based latency scoring

High-frame-rate video and frame-by-frame scoring improve the reliability of time-to-turn and time-to-descend measurement and reduce observer burden.

Methods

Separate turn and descent endpoints

Reporting time to turn and time to descend separately is increasingly expected because each captures a distinct stage of the motor sequence.

Emerging

Multi-assay motor batteries

The pole test is paired with rotarod, gait analysis, and grip strength to separate bradykinesia from coordination, endurance, and strength.

§ 5

Background references

5 selected methods and validation studies of the Pole Test, from any lab.

  1. Ogawa N, Hirose Y, Ohara S, Ono T, Watanabe Y. A simple quantitative bradykinesia test in MPTP-treated mice. Res Commun Chem Pathol Pharmacol. 1985;50(3):435-441. PMID:4089123
  2. Matsuura K, Kabuto H, Makino H, Ogawa N. Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion. J Neurosci Methods. 1997;73(1):45-48. doi:10.1016/s0165-0270(96)02211-x
  3. Fleming SM, Salcedo J, Fernagut PO, et al. Early and progressive sensorimotor anomalies in mice overexpressing wild-type human alpha-synuclein. J Neurosci. 2004;24(42):9434-9440. doi:10.1523/JNEUROSCI.3080-04.2004
  4. Sedelis M, Schwarting RK, Huston JP. Behavioral phenotyping of the MPTP mouse model of Parkinson's disease. Behav Brain Res. 2001;125(1-2):109-125. doi:10.1016/s0166-4328(01)00309-6
  5. Brooks SP, Dunnett SB. Tests to assess motor phenotype in mice: a user's guide. Nat Rev Neurosci. 2009;10(7):519-529. doi:10.1038/nrn2652
Pole Test
Pole Test
$690.00
Added to quoteView Quote