Behavioral Mazes

Pole Test

$490.00 - $690.00

Vertical pole apparatus for assessing motor coordination and movement disorders in rodent models of Parkinson’s disease and dopaminergic dysfunction.

Species SKU 5019
$690.00
Key Specifications
warranty_length
1 YEAR
storage_included
Yes
assembly_required
Yes
Automation Level
manual
Compatible Tracking Software
ConductVision
SKU:5019
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Scientist guidance
Louise Corscadden, PhD, Director of Science

Louise Corscadden, PhD

Director of Science · ConductScience

Ask Louise about Pole Test fit, setup, configuration, or quote prep.

The Pole Test is a standardized behavioral apparatus designed to assess motor coordination and movement disorders in rodents. This simple yet sensitive test evaluates an animal's ability to orient and descend from a vertical pole, providing quantitative measures of motor dysfunction commonly associated with nigrostriatal damage and dopaminergic system impairment.

The apparatus consists of a vertical pole with species-specific diameters (8 mm for mice, 10 mm for rats) that allows researchers to measure turning time, total descent time, and movement quality. The test is particularly valuable for evaluating motor phenotypes in Parkinson's disease models, including 6-OHDA lesioned animals and MPTP-treated subjects, where bradykinesia and postural instability are primary endpoints.

*TUB NOT INCLUDED

How It Works

The pole test exploits the natural tendency of rodents to turn head-down and descend when placed on a vertical pole. Normal animals rapidly orient themselves and descend smoothly, while animals with motor dysfunction exhibit prolonged turning times and hesitant, bradykinetic movements during descent.

The test measures two primary parameters: turning time (latency to orient head-down) and total descent time (time to reach the base). Additional qualitative assessments include movement fluidity, number of slips, and use of alternative descent strategies. These measures correlate with the degree of striatal dopamine depletion and provide a sensitive index of motor impairment.

The apparatus diameter is optimized for each species to ensure appropriate grip difficulty - too narrow causes slipping in healthy animals, while too wide fails to challenge motor-impaired subjects. The standardized dimensions (8 mm for mice, 10 mm for rats) have been validated across multiple Parkinson's disease models.

Features & Benefits

Species-specific pole diameters
Optimized grip challenge for mice (8 mm) and rats (10 mm) ensures appropriate difficulty level for motor assessment
Standardized vertical design
Consistent test conditions enable reliable comparison across studies and laboratories
Simple apparatus construction
Minimal technical requirements allow focus on behavioral endpoints rather than equipment complexity
Quantitative timing measures
Objective assessment of turning time and descent time provides statistical power for group comparisons
Compatible with video analysis
Supports automated scoring and detailed movement analysis for comprehensive motor evaluation
Storage container included
Organized storage prevents component loss and maintains apparatus condition between experiments
Assembly required design
Compact shipping and secure setup ensure apparatus stability during testing

Accessories

Enhance your setup with compatible accessories

Total: $0.00

Frequently Bought Together

Total: $2,730.00

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The complete Pole Test workflow

Track behavior

No exact ConductVision pole-test page is currently published. Time to turn and time to descend are normally captured by a stopwatch and frame-by-frame video rather than overhead tracking; keep automated turn detection as a roadmap gap.

Supporting page not yet built

Run protocol

Training trials, pole texture and diameter, head-up orientation, and definitions for time to turn, time to descend, falls, and missteps.

ConductMaze Pole Test Protocol ->

Analyze output

Summarize time to turn, time to descend, falls and slips, missteps, and turn-direction bias across trials with quality-control flags.

Pole Test Latency Scorer ->

Configuration considerations

Common Pole Test setup decisions

Use these notes to scope species, cohort, tracking, and automation needs. Only verified product or support routes are linked from this section.

This productTextured pole

Vertical Pole Apparatus

Vertical textured pole on a base with a home cage at the bottom and padding around it

Standard configuration for bradykinesia and motor initiation, scoring time to turn and time to descend as the animal turns head-down and climbs to the base.

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BuyableMouse or rat

Species-Scaled Pole

Pole diameter and height scaled for mouse or rat grip and body size

Pole diameter and height change grip mechanics and descent time, so the pole geometry should match the grip span and body size of the species being tested.

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SpecialtyGauze-wrapped

High-Grip Pole

Gauze-wrapped pole surface for maximal grip and reduced slipping

Best when slipping confounds descent time, because a gauze-wrapped high-grip surface isolates turning and descent coordination from grip failure on a smooth pole.

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§ 1

Introduction

The Pole Test measures bradykinesia and motor initiation by recording how long a rodent takes to turn head-down at the top of a vertical pole and to descend to the base. Ogawa and colleagues introduced the test as a simple quantitative measure of bradykinesia in dopamine-depleted mice. 1

The two core readouts are time to turn, an index of movement initiation, and time to descend, which captures the full turning-and-climbing sequence. Because turning head-down on a narrow pole requires coordinated initiation and postural adjustment, the test is widely used to characterize sensorimotor anomalies in models of striatal dopamine depletion. 1

Pole texture and diameter, training state, body weight, motivation to descend to the home cage, and motor learning all change turn and descent times independent of true motor initiation. A defensible protocol fixes pole geometry and surface, trains animals to a stable head-up start, scores falls and missteps separately, and reports turn-direction bias as a quality-control flag. 1

§ 2

Methods

2.1 Procedure

Head-up start with time-to-turn and time-to-descend scoring, fall and misstep classification, and turn-direction tracking.

Pre-test setup

  1. 1.Acclimation and habituationHabituate animals to the room and to the home cage at the base of the pole so the first measured trial reflects motor initiation rather than novelty or handling stress.
  2. 2.Apparatus calibrationVerify pole diameter, height, and surface texture, place the home cage and padding at the base, and confirm the camera captures the turn and full descent for frame-by-frame scoring.
  3. 3.Training to baselineTrain animals over consecutive trials to start head-up at the top and turn reliably, so test-day data reflect motor initiation rather than learning the task.
  4. 4.Define scoring rulesPre-define time to turn, time to descend, what counts as a fall, what counts as a misstep, and how trials are capped for animals that slide rather than climb.

Trial sequence

  1. 1.Place head-up at the topPosition the animal head-up near the top of the pole and release it, starting the timer at release.
  2. 2.Record time to turnRecord the time from release until the animal completes a head-down turn, the index of movement initiation.2
  3. 3.Record time to descendRecord the total time from release until all four paws reach the base, capping at the pre-defined maximum for animals that stall or slide.3
  4. 4.Classify falls and misstepsRecord falls and missteps as distinct outcomes rather than folding them into descent time, since each reflects a different coordination or grip failure.
  5. 5.Log turn bias and repeatNote turn direction to flag a directional bias, then clean the pole between subjects to remove odor and urine cues before the next trial.

Critical methodological constraints

  • Pole texture and diameter. Surface texture and diameter set grip and descent difficulty. A smooth pole causes sliding that confounds descent time; geometry and surface must be held constant.2
  • Training state. Untrained animals confound motor initiation with task acquisition. Train to a stable head-up start and reliable turn before the test session.3
  • Body weight. Heavier animals descend differently independent of motor initiation. Report body weight and consider it when groups differ in mass.
  • Motivation. A home cage or familiar enclosure at the base motivates descent. A weak incentive increases stalling and inflates descent time without a true deficit.

2.2 Measurement & Analysis

Core pole-test endpoints for bradykinesia, motor initiation, and quality control.

Time To Turn (T-turn)

Bradykinesia

Time from release until the animal completes a head-down turn at the top of the pole, the primary index of movement initiation.2

Time To Descend (T-total)

Motor initiation & descent

Total time from release until all four paws reach the base, capturing the full turning-and-climbing sequence.3

Falls/Slips

Coordination failure

Number of falls or slips down the pole, recorded as a distinct outcome rather than folded into descent time.

Missteps

Grip control

Count of paw missteps or slips during the controlled descent, indexing grip and limb-placement control.

Turn Direction Bias

Quality-control flag

Consistent preference for turning one direction, which can flag unilateral motor asymmetry or an apparatus artifact.

+ Additional metrics: trial number, pole surface, body weight, latency to start moving, descent strategy (climb versus slide), and per-trial video notes.

2.3 descent-time fraction (analysis)

A compact fraction of the maximum trial window taken up by turning and descending.

Inline calculator

Type the values your tracker recorded.

Full calculator with 95% CI ->
Descent-time fraction

30.0%

Formula: turn + descent time / (turn + descent time + max-trial remaining) x 100. Interpret with pole texture, training state, body weight, and fall or misstep counts because a long descent can reflect sliding or grip failure rather than bradykinesia. 1

2.4 sample-size planning

Estimate the N per group needed to detect a literature-anchored motor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.

sample-size planning

Estimate the N per group needed to detect a literature-anchored motor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.

Striatal dopamine-depleted vs control mouse on a textured pole; representative magnitudes from Matsuura et al. (1997) pole-test validation.2

Cohen's d

2.14

N per group at 80% power

4

Total N

8

With attrition cushion

9

At 70% / 90% power

3 / 5

Methods sentence

Need ANOVA, proportions, paired design, or a power curve? Open in the full Sample-Size Calculator →

Formula: n = 2 · ((zα/2 + zβ) / d)2, where d = |μ₁ − μ₂| / σ. Assumes equal allocation, normality, and homoskedasticity. The attrition cushion inflates total N by 1 / (1 − dropout); confirm with your IACUC.

§ 3

Results

Aggregate publication data, sample apparatus output, and recent findings from the live PubMed feed.

3.1 Publication trends

PubMed volume and co-occurring behavioral methods for pole-test bradykinesia studies.

Figure 1 · EPM publications by year (PubMed)

The paradigm has been dominant for 40 years and is still growing.

Live · Weekly

2000201020202025 YTD: 67 papers

Total in PubMed since 1985: 1,420+ papers. Updated 2026-06-12.

Figure 2 · Methods co-occurring with EPM (last 12 months)

Other paradigms most often run alongside EPM in the same paper.

Live

3.2 Sample apparatus output

Representative output from a textured-pole session with a head-up start and a 30 s trial cap.

Table 1 · Per-animal EPM scoring output

Download sample CSV →
AnimalGroupTime to turnTime to descendFallsDescent-time fraction
PT-001Control2.0 s8.2 s027.3%
PT-002Control1.9 s7.6 s025.3%
PT-003Control2.4 s9.1 s030.3%
PT-004Parkinsonian6.5 s17.4 s158.0%
PT-005Parkinsonian7.2 s19.0 s263.3%
PT-006Parkinsonian6.8 s18.2 s260.7%

Synthetic example for illustration only. Score falls and missteps distinctly and check pole surface before interpreting descent times as bradykinesia.

3.3 Recent findings (live PubMed feed)

  • Jun 2026Source note

    Pole-test methods continue to emphasize textured pole surfaces and separate turn and descent endpoints.

    Static methods note aligned with Ogawa et al. (1985), Matsuura et al. (1997), and Fleming et al. (2004).

    Review pole-test studies for a fixed pole surface and diameter, training to a stable head-up start, falls and missteps scored separately, and time to turn reported distinctly from time to descend before interpreting bradykinesia.

    Methods overviewReproducibility
  • Jun 2026Source note

    Pole test as one assay in a motor battery: pair with rotarod, gait analysis, and grip strength.

    Static methods note aligned with Sedelis et al. (2001) and Brooks & Dunnett (2009).

    A single descent time is a screening signal. Bradykinesia is most defensible when confirmed with separate turn and descent endpoints and an independent motor assay scored in the same cohort.

    Motor batteryBradykinesia

View all 1420matching papers on PubMed ->

§ 4

Discussion

Limitations of the paradigm, methodological caveats, and current directions.

4.1 Common confounds

Variables that shift Pole Test results independent of anxiety state.

Pole texture/diameter

Surface texture and diameter set grip and descent difficulty. A smooth pole causes sliding that confounds descent time, so geometry and surface must be held constant.

Training state

Untrained animals confound motor initiation with task acquisition. Train to a stable head-up start and reliable turn before testing.

Body weight

Heavier animals descend differently independent of motor initiation, so weight should be reported and considered when groups differ in mass.

Motivation (home-cage at base)

A home cage or familiar enclosure at the base motivates descent. A weak incentive increases stalling and inflates descent time without a true deficit.

Motor learning

Turn and descent times improve across early trials as animals learn the task, so a single session can confound bradykinesia with task acquisition.

Confound checklist

Tick the confounds your protocol addresses, then export a methods-paragraph blurb you can paste into your manuscript.

Preview exported markdown
## Pole Test — methods controls

Confounds controlled in this protocol:

- **Pole texture/diameter.** Surface texture and diameter set grip and descent difficulty. A smooth pole causes sliding that confounds descent time, so geometry and surface must be held constant.
- **Training state.** Untrained animals confound motor initiation with task acquisition. Train to a stable head-up start and reliable turn before testing.
- **Body weight.** Heavier animals descend differently independent of motor initiation, so weight should be reported and considered when groups differ in mass.
- **Motivation (home-cage at base).** A home cage or familiar enclosure at the base motivates descent. A weak incentive increases stalling and inflates descent time without a true deficit.
- **Motor learning.** Turn and descent times improve across early trials as animals learn the task, so a single session can confound bradykinesia with task acquisition.

4.2 Construct validity caveats

The pole test is strongest when pole surface and geometry, training, and scoring rules are fixed before testing, and falls and missteps are recorded separately from descent time. Time to turn and time to descend index different stages; report them separately and confirm bradykinesia with an independent motor assay such as gait analysis or the rotarod. 1

4.3 Special considerations

When should I use the rotarod instead?

Use the rotarod for gross coordination and endurance under forced locomotion. The pole test is more specific to movement initiation and bradykinesia, especially in models of striatal dopamine depletion.

Why separate time to turn from time to descend?

Time to turn indexes movement initiation while time to descend captures the full turning-and-climbing sequence. Reporting them separately distinguishes an initiation deficit from a descent or grip problem.

How do I prevent sliding from confounding descent time?

Use a textured or gauze-wrapped pole so animals climb rather than slide. A smooth surface lets animals slide down, which shortens descent time without reflecting coordinated motor control.

4.4 Current directions

Quarterly editorial review of emerging Pole Test methodology. Q2 2026

Methods

Standardized pole surface

Specifying pole texture and diameter improves comparability of turn and descent times between labs and reduces sliding artifacts.

Emerging

Video-based latency scoring

High-frame-rate video and frame-by-frame scoring improve the reliability of time-to-turn and time-to-descend measurement and reduce observer burden.

Methods

Separate turn and descent endpoints

Reporting time to turn and time to descend separately is increasingly expected because each captures a distinct stage of the motor sequence.

Emerging

Multi-assay motor batteries

The pole test is paired with rotarod, gait analysis, and grip strength to separate bradykinesia from coordination, endurance, and strength.

§ 5

References

5 selected methods and validation references for Pole Test.

  1. Ogawa N, Hirose Y, Ohara S, Ono T, Watanabe Y. A simple quantitative bradykinesia test in MPTP-treated mice. Res Commun Chem Pathol Pharmacol. 1985;50(3):435-441. PMID:4089123
  2. Matsuura K, Kabuto H, Makino H, Ogawa N. Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion. J Neurosci Methods. 1997;73(1):45-48. doi:10.1016/s0165-0270(96)02211-x
  3. Fleming SM, Salcedo J, Fernagut PO, et al. Early and progressive sensorimotor anomalies in mice overexpressing wild-type human alpha-synuclein. J Neurosci. 2004;24(42):9434-9440. doi:10.1523/JNEUROSCI.3080-04.2004
  4. Sedelis M, Schwarting RK, Huston JP. Behavioral phenotyping of the MPTP mouse model of Parkinson's disease. Behav Brain Res. 2001;125(1-2):109-125. doi:10.1016/s0166-4328(01)00309-6
  5. Brooks SP, Dunnett SB. Tests to assess motor phenotype in mice: a user's guide. Nat Rev Neurosci. 2009;10(7):519-529. doi:10.1038/nrn2652
Pole Test
Pole Test
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