Vertical Pulse Vacuum Autoclave
50-liter vertical autoclave with pulse vacuum system, operating at 105-134°C with ±0.1°C precision for laboratory sterilization applications.
Louise Corscadden, PhD
Director of Science · ConductScience
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Key Specifications
Full details →- Model fit
- Configured during quote
- SKU family
- BIO-0148
- Sizing
- 107.0 x 70.0 x 61.0 cm
- Ordering
- Online checkout and quote request available
- Category
- Autoclaves
- Build notes
- S30408
The Vertical Pulse Vacuum Autoclave provides laboratory-grade steam sterilization with precise temperature control and vacuum-assisted air removal. This 50-liter capacity unit operates at working pressures up to 0.23 MPa with temperature precision of ±0.1°C across the 105-134°C working range. The vertical chamber design with S30408 stainless steel construction accommodates standard laboratory glassware, media, and instruments.
The integrated vacuum system enables effective air displacement for enhanced steam penetration, critical for sterilizing porous materials and complex geometries. The unit features an eject vacuum system with noise levels maintained below 65 dB(A) for laboratory environments. Power consumption of 5.5 KW supports rapid heating cycles while maintaining temperature uniformity throughout the 386mm diameter by 490mm height chamber.
How It Works
Steam sterilization operates through moist heat transfer, where saturated steam at elevated temperature denatures proteins and disrupts cellular membranes in microorganisms. The vertical pulse vacuum system creates alternating vacuum and steam injection cycles, removing air pockets that would otherwise insulate materials from steam contact. This process ensures uniform temperature distribution throughout the load.
The S30408 stainless steel chamber withstands repeated thermal cycling while maintaining structural integrity. Temperature sensors monitor the chamber environment, maintaining working temperatures between 105-134°C with ±0.1°C precision. The vacuum system removes residual air before steam introduction, then evacuates moisture after sterilization to facilitate drying.
Sterilization kinetics follow first-order kinetics, where microbial death rate depends on temperature and time exposure. The combination of saturated steam, elevated temperature, and vacuum-enhanced penetration achieves sterility assurance levels appropriate for laboratory applications.
Features & Benefits
Automation Level
- semi-automated
Designed Pressure
- -0.1/0.28MPa
Working pressure
- 0.23MPa
Designed Temp.
- 150℃
Working Temp.
- 105~134℃
Temp. Precision
- 0.1℃
Chamber Material
- S30408
Chamber Size
- φ386*490mm
Basket
- φ360*260mm, 1pc
Vacuum System
- Eject vacuum system
Noise
- ≤65dB(A)
Altitude
- ≤2000m, could be customized at 2000~3000m
Consumption
- 5.5KW
Brand
- ConductScience
Research Domain
- Analytical Chemistry
- Cell Biology
- Environmental Monitoring
- Food Science
- Microbiology
- Pharmaceutical QC
Capacity
- 50L
Power/Voltage
- AC220V, 50/60Hz(Standard); AC110V, 50/60Hz(Optional)
Weight
- 127.0 kg
Dimensions
- L: 107.0 mm
- W: 70.0 mm
- H: 61.0 mm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Chamber Capacity | 50 liters with 386mm × 490mm dimensions | Entry-level models often offer 20-30 liter capacity | Larger capacity reduces the number of sterilization cycles needed for high-throughput laboratories. |
| Vacuum System | Integrated pulse vacuum with eject system | Basic models rely on gravity displacement only | Vacuum-assisted air removal ensures reliable sterilization of hollow instruments and porous materials. |
| Temperature Precision | ±0.1°C precision across 105-134°C range | Standard units typically offer ±1-2°C precision | Enhanced precision supports validated protocols requiring tight temperature control for regulatory compliance. |
| Chamber Material | S30408 stainless steel construction | Basic models may use lower-grade steel alloys | Superior corrosion resistance extends equipment lifespan and reduces maintenance requirements. |
| Operating Pressure | 0.23 MPa working pressure, designed for -0.1/0.28 MPa | Entry-level units often limited to lower pressure ranges | Higher pressure capability enables more aggressive sterilization cycles for difficult-to-sterilize materials. |
| Noise Level | ≤65 dB(A) operation | Varies by model, often higher noise levels | Quieter operation maintains comfortable laboratory working conditions during extended sterilization cycles. |
This autoclave combines substantial 50-liter capacity with precision temperature control and vacuum-enhanced sterilization capabilities. The vertical design optimizes space utilization while the S30408 stainless steel construction and quiet operation support demanding laboratory environments.
Practical Tips
Verify temperature accuracy using calibrated thermocouples placed at multiple chamber locations during monthly calibration checks.
Why: Temperature uniformity validation ensures reliable sterilization throughout the chamber volume.
Clean chamber walls and door gaskets daily with appropriate detergents, then rinse thoroughly before next use.
Why: Residue buildup can interfere with vacuum performance and create potential contamination sources.
Leave space between items in the basket to allow steam circulation, avoiding tight packing that could create cold spots.
Why: Adequate steam flow ensures uniform heat transfer to all surfaces being sterilized.
If vacuum performance decreases, check door seal integrity and vacuum system filters for blockages or wear.
Why: Poor vacuum performance directly impacts air removal and sterilization efficacy.
Record chamber temperature, pressure readings, and cycle times for each sterilization run to maintain validation records.
Why: Documentation provides evidence of proper sterilization conditions for quality assurance and regulatory requirements.
Allow chamber pressure to return to atmospheric before opening door, and use appropriate PPE when handling hot materials.
Why: Pressurized chambers and superheated materials pose serious injury risks if proper safety procedures are not followed.
Pre-warm the chamber for 10-15 minutes before loading heat-sensitive materials to reduce thermal shock.
Why: Gradual temperature changes prevent cracking of glassware and thermal stress on delicate materials.
Inspect and replace door gaskets annually or when signs of wear become apparent during routine inspections.
Why: Compromised seals reduce vacuum efficiency and can lead to incomplete sterilization cycles.
Setup Guide
What’s in the Box
- Vertical pulse vacuum autoclave main unit
- Stainless steel basket (360mm × 260mm)
- Power connection cables
- Water supply fittings (typical)
- Operating manual and safety documentation
- Warranty registration materials (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering defects in materials and workmanship, with technical support available for installation guidance and operational questions.
Compliance
What is the maximum load capacity for glassware sterilization?
The 50-liter chamber with 386mm diameter × 490mm height accommodates standard laboratory glassware sets. Consult product datasheet for specific weight limits and loading configurations based on material density and steam circulation requirements.
How does the pulse vacuum system improve sterilization efficacy?
The eject vacuum system creates alternating vacuum and steam injection phases, removing air pockets that could prevent steam contact with surfaces. This is particularly important for hollow instruments, textiles, and complex geometries where trapped air would compromise sterilization.
What cycle times are typical for different load types?
Cycle times depend on load composition, density, and required sterility assurance level. Consult product datasheet for recommended time-temperature profiles for specific materials, as these vary significantly between aqueous solutions, glassware, and porous materials.
Can this autoclave handle heat-sensitive culture media?
The 105-134°C temperature range with ±0.1°C precision allows selection of appropriate conditions for different materials. Lower temperatures around 105-115°C may be suitable for some heat-sensitive media, though specific compatibility should be verified experimentally.
What maintenance schedule is recommended?
Regular maintenance includes daily chamber cleaning, weekly vacuum system checks, and periodic calibration verification. Consult manufacturer specifications for detailed maintenance intervals and procedures specific to usage frequency and load types.
Is the temperature uniformity validated throughout the chamber?
The ±0.1°C precision specification suggests temperature control capability, but specific uniformity mapping data should be requested from the manufacturer. Validation typically requires multi-point temperature mapping during qualification.
What power supply considerations are needed for installation?
The 5.5 KW power requirement necessitates dedicated electrical circuits rated appropriately for this load. Standard AC220V 50/60Hz supply is typical, with AC110V 50/60Hz available as an option for North American installations.
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