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Introduction Allosteric enzymes are a group of biocatalysts that possess common characteristics of an enzyme but do not exhibit a typical Michaelis-Menten kinetic behavior. Instead,
In the past, bacteriophage λ DNA purification was largely achieved through procedures that involved Cesium chloride gradients. However, these methods had a low recovery rate
Introduction Genomic DNA or cDNA clones are analyzed in a simple procedure that begins with the restriction digestion of phage DNA mini preparations. The digested
Introduction and Principle Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with
Introduction The vectors derived from filamentous phages containing a plasmid’s origin of replication are called phagemids (Qi et al., 2012). Phagemids comprise typical high-copy-number plasmids
Need Digital Health Services for your research? Click here Filters carrying immobilized bacteriophage DNA are labeled with 32P-labelled probes to screen by hybridization in situ.
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