Anesthesia Induction Chamber

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Conductscience’s anesthesia box is a state of the art apparatus contrived from supreme quality acrylic. It is used to confine subjects during an induction procedure. Our anesthesia box comes with a top sliding lid for the convenient entry and removal of the subjects. The box is provided with two adjacent orifices; inlet for the entry of the fresh anesthetizing gas and an outlet for scavenging the waste gas material. The transparent material helps in visualizing the physical state of the subject; thus preventing accidental overdosing.

The anesthesia box should be placed on an even surface with the opening away from the experimenter so that the sliding lid slides open toward the experimenter. The subject(s) should be carefully placed into the box, and the supply of fresh anesthetizing should be initiated and maintained for approximately 2 to 5 minutes. After the subject is fully anesthetized, anesthesia supply should be discontinued. Critical: Do not slide open the anesthesia box immediately, wait for about 10 to 15 seconds so that oxygen in the box neutralizes the gas concentration.

Then the box should be lifted at the end closest to the experimenter to gently slide the anesthetized subject to the far end of the box. Finally, the sliding lid should be opened slightly, just enough to remove the anesthetized subject from the box, and closed immediately.

Caution: Do not slide the lid wide open, just enough so that a hand can be introduced for the removal of the subject.

By keeping the opening far away from the experimenter and limiting the time that the box is open, the risk of exposure to the experimenter will be diminished.

Our exquisite design allows the following benefits:

  • Our design is preferred over the conventional mask and circuits because it is less time consuming and it can hold and anesthetize more than one subjects simultaneously.
  • Our special design provides extra protection to the experimenter by keeping the opening far away from the experimenter.
  • Our device is compatible with almost all (non-explosive) gas mixtures. However, it should not be used with liquid organic solvents.
  • Our scavenging tube efficiently removes the waste gas materials.

Specifications

RWD-V100Induction Chamber, Mouse, 15cm*10cm*10cm
RWD-V101Induction Chamber, Rat, 24cm*12cm*18cm
RWD-V102Induction Chamber, Cat/Rabbit, 40cm*18.5cm*25cm
RWD-V103Induction Chamber, Large Animal, 50cm*30cm*30cm

Documentation

A fundamental component of surgical procedures in animal research is the usage of anesthetics. Anesthesia is an important tool to restraint animals during procedures that may either cause excessive stress to the animal or expose the researcher to unavoidable hazards, as well as post-surgery pain management. The surgical methods that are sure to cause pain or discomfort in animals must, therefore, be performed under general anesthesia (“Anesthesia and Analgesia in Laboratory Animals,” 2010). Anesthesia may be inhaled or injected depending on factors such as type and duration of surgery and animal species.

The process of putting animals under inhalant anesthesia is made easier with the use of anesthesia box that confines subjects during the anesthesia induction procedure. The anesthesia box comes with a top sliding lid for convenient entry and removal of subjects. It is also provided with two separate openings, an inlet for the entry of fresh anesthetizing gas and outlet for scavenging the waste gas material. To prevent accidental overdosing, the apparatus is made of transparent acrylic for easy observation of the animal.

Apparatus and Equipment

The anesthesia box is a state of the art apparatus manufactured from supreme quality acrylic, used to enclose animals during inhalant anesthesia induction procedures. The apparatus is a simple, transparent box structure with a top sliding lid for convenient entry and removal of rodent or other animal subjects, along with two separate openings, one for the entry of fresh anesthetizing gas and one for scavenging the waste gas material. The transparent acrylic material is used for convenient inspection of the animal’s physical state, in order to prevent any possibility of accidental overdose.

Protocol

The anesthesia box must be carefully placed on an even and stable surface, with the top sliding lid displacing towards the experimenter, placing the opening on the far side to avoid accidental escape and inhalation of gas. Depending on its size, the anesthesia box may accommodate more than one animal.

 

Once the animal is carefully placed inside the empty anesthesia box, the supply of fresh anesthetizing gas is then initiated and maintained for approximately 2 to 5 minutes. The supply should then be discontinued once the animal is fully anesthetized. A critical step is to wait for about 10 to 15 seconds after the anesthesia supply is cut off so that the oxygen in the box neutralizes the gas concentration. Afterward, the anesthesia box is then moved in such a way that the animal slides toward the side of the opening. Once the animal is retrieved through a slight opening in the top lid, the anesthesia box is immediately closed to prevent unwanted escape of gas. Once the animal is moved out of the chamber, anesthesia may be maintained via other tools, such as the cone device.

Applications

The discovery of general anesthesia during the middle of the 19th century can be considered one of the most important developments in the history of medicine (Werner et al., 2011), and since then, its usefulness has found other important applications, such as animal research.

Much of research in the neurosciences is benefited by the use of experimental animals. A huge aspect of animal research is the employment of surgical procedures, and ethical considerations necessitate anesthesia as a tool for eliminating any unnecessary stress, pain, or discomfort the animal may experience during and after surgery. Today, there are many novel and sophisticated methods for administering different types of anesthesia, but the mechanisms have not changed. The anesthesia box presents a convenient way of employing gas induction procedures, with such substances as isoflurane, sevoflurane, and other inhalant agents, that, through inlet and outlet openings of gas and a transparent, sealed design, ensures the safety of everybody involved.

Strengths and Limitations

The anesthesia box’s design is much preferred over the conventional mask and circuit method because it requires less time for the inhalant anesthesia to take effect. Further, the anesthesia box can hold and anesthetize more than one animal simultaneously, depending on its size.

It is a reality that exposure to waste anesthetic gas is a serious occupational hazard (“Anesthesia and Analgesia in Research Animals,” 2012). The experimenter’s safety is, therefore, another concern which the anesthetic box’s design succeeds in addressing. The anesthesia box’s special design provides extra protection to the experimenter by keeping the opening on the farther side to avoid exposure. Additionally, the anesthesia box is compatible with most non-explosive gas mixtures. The outlet scavenging tube is also an efficient addition to the anesthesia box’s design, effectively removing toxic waste gas materials.

Summary

  • An anesthesia box is a tool used for confining animal subjects in a closed space during anesthesia induction procedures
  • The anesthesia box comes with a top sliding lid for convenient entry and removal of subjects, and two separate inlet and outlet openings for the entry of fresh anesthetizing gas and collection of scavenging waste gas material
  • To prevent accidental overdosing, the apparatus is made of transparent acrylic for easy observation of the physical state of the animal
  • The anesthesia box ensures the safety of experimenters from accidental exposure to waste anesthetic gas

References

Anesthesia and Analgesia in Laboratory Animals. (2010, November 08). Retrieved from Penn State Animal Research Program, https://www.research.psu.edu/arp/anesthesia.html

Anesthesia and Analgesia in Research Animals. (2012, December 01). Retrieved from https://lar.indiana.edu/doc/Anesthesia_and_Analgesia_in_Research_Animals.pdf

Werner, D. F., Swihart, A., Rau, V., Jia, F., Borghese, C. M., McCracken, M. L., … & Eger, E. I. (2011). Inhaled anesthetic responses of recombinant receptors and knockin mice harboring α2 (S270H/L277A) GABAA receptor subunits that are resistant to isoflurane. Journal of Pharmacology and Experimental Therapeutics, 336(1), 134-144.

Quan Ren, Mian Peng, Yuanlin Dong, Yiying Zhang, Ming Chen, Ning Yin, Edward R. Marcantonio, Zhongcong Xie. (2015). Surgery plus anesthesia induces loss of attention in mice. Front Cell Neurosci, 9: 346

Amy Miller, Gemma Kitson, Benjamin Skalkoyannis, Matthew Leach. (2015). The effect of isoflurane anaesthesia and buprenorphine on the mouse grimace scale and behaviour in CBA and DBA/2 mice. Appl Anim Behav Sci, 172: 58–62

Lundt A, Wormuth C, Siwek ME, Muller R, Ehninger D, Henseler C. (2016). EEG Radiotelemetry in Small Laboratory Rodents: A Powerful State-of-the Art Approach in Neuropsychiatric, Neurodegenerative, and Epilepsy Research. Neural Plast, 8213878

F. Werner, A. Swihart, V. Rau, F. Jia, C. M. Borghese, M. L. McCracken, S. Iyer, M. S. Fanselow, I. Oh, J. M. Sonner, E. I. Eger, N. L. Harrison, R. A. Harris, G. E. Homanics. (2011). Inhaled Anesthetic Responses of Recombinant Receptors and Knockin Mice Harboring α2(S270H/L277A) GABAA Receptor Subunits That Are Resistant to Isoflurane. Journal of Pharmacology and Experimental Therapeutics January, 336 (1) 134-144

Michael A. Makara, Ky V. Hoang, Latha P. Ganesan, Elliot D. Crouser, John S. Gunn, Joanne Turner, Larry S. Schlesinger, Peter J. Mohler, Murugesan V.S. Rajaram. (2016). Cardiac Electrical and Structural Changes During Bacterial Infection: An Instructive Model to Study Cardiac Dysfunction in Sepsis. Journal of the American Heart Association, 5:e003820

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Size

Large Animal, Mouse, Rabbit/Cat, Rat