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Lab Basics

The light digital microscope can be used to visualize structures as small as ~1 micron
Lab BasicsMicroscopy
May 27, 2019

Fluorescence Microscopy

Introduction Fluorescence microscopy is a specialized type of light microscopy that relies on the phenomena of fluorescence or phosphorescence. These terms describe materials that absorb light (photons) of one wavelength, then re-emit light of a second, longer wavelength. The difference between fluorescence and phosphorescence is that the latter lasts much…
Kevin Hughes
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The light digital microscope can be used to visualize structures as small as ~1 micron
Lab BasicsMicroscopy
May 24, 2019

Scanning Tunneling Microscope

Introduction Scanning Tunneling Microscopy, or STM, is a scanning probe technique. This means that the specimen is imaged by scanning a very sharp tip over its surface, while information about the shape or composition of the surface is collected based on interactions between the tip and the sample. This is…
Kevin Hughes
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The light digital microscope can be used to visualize structures as small as ~1 micron
Lab BasicsMicroscopyProtocolsScience
May 18, 2019

Optical Microscopy: Specimen Preparation, Staining, and Quantitative Analysis

Introduction In most types of microscopy, the most complicated and sensitive aspect of the analysis is the preparation of specimens. The visualization of microorganisms using optical microscopy is no exception. Microorganisms are generally transparent, fragile, and highly sensitive to their environments, and for that reason, careful preparation is required to…
Kevin Hughes
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The light digital microscope can be used to visualize structures as small as ~1 micron
Lab BasicsMicroscopyScience
April 25, 2019

Scanning electron microscopy (SEM)

Introduction The scanning electron microscope employs a focused beam of high-energy electrons to visualize the regions of interest on the surface of solid specimens. The signals from electron-sample interactions unveil the information about the sample such as its morphology, chemical composition, and crystalline structure and material. The SEM can image…
Conductscience
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The light digital microscope can be used to visualize structures as small as ~1 micron
Microscopy
April 25, 2019

Electron Microscopy

Introduction Electron microscope is an imaging instrument that uses a beam of energetic electrons to observe objects on a very fine scale. Electron Microscopes were developed to overcome the limitations of light microscope which can only visualize the specimens with 500x or 1000x magnification and 0.2 micrometers resolution. In the…
Conductscience
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