Barnes Maze (BM) is a behavioral task often used in neuroscience for the study of spatial learning and memory. The task’s primary ability is to measure the capacity of the subject to learn the location of the target by using distal visual cues. The maze exploits the averseness that rodents feel towards open and brightly lit spaces to motivate them to find the target location. The Barnes Maze requires the use of hippocampal-dependent spatial reference memory to be able to locate the escape locations. This ability to remember the location of the target hole can be affected by the administration of certain drugs or in disease models.