MAILbox: A Message to All Intelligent Lifeforms
Want to be part of our Stories? Tell us your story Click here Name: Suchetan Mummigatti Location: Cranfield, United Kingdom Graduation Date: September 11, 2020 Hello! Who are you and what are you working on? I have a Bachelor’s degree in Aerospace Engineering (Bangalore, India) and a Master’s in Astronautics and Space Engineering from Cranfield University, UK. My […]
Allosteric Enzymes: Characteristics, Models, and Examples
Introduction Allosteric enzymes are a group of biocatalysts that possess common characteristics of an enzyme but do not exhibit a typical Michaelis-Menten kinetic behavior. Instead, their kinetics follow a sigmoid curve. The key to allosteric enzyme activity lies in the reversible binding to a specific molecule on a non-catalytic site of the enzyme. The binding […]
Bacteriophage λ DNA Purification from Liquid Cultures
In the past, bacteriophage λ DNA purification was largely achieved through procedures that involved Cesium chloride gradients. However, these methods had a low recovery rate and a high chance of the presence of contaminants. Modern bacteriophage λ DNA purification methods give high yield and provide DNA free from contamination (Pickard, 2009). We can also purify […]
Bacteriophage λ DNA Purification from Plate Lysates
Introduction Genomic DNA or cDNA clones are analyzed in a simple procedure that begins with the restriction digestion of phage DNA mini preparations. The digested DNA is then assessed by agarose gel electrophoresis. For the rapid analysis, one can purify bacteriophage DNA from plate lysates as well as liquid cultures. This method explains the purification […]
Cloning into Bacteriophage M13 Vectors
Introduction and Principle Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with cohesive termini into compatible sites in M13 double-stranded RF DNA. Transforming ligation products into competent male E. coli cells (plated in top agar supplemented with X-gal and IPTG. Picking and […]
Generation of Single-Stranded DNA with Phagemid Vectors
Introduction The vectors derived from filamentous phages containing a plasmid’s origin of replication are called phagemids (Qi et al., 2012). Phagemids comprise typical high-copy-number plasmids equipped with a major intergenic region (508bp in length) of a filamentous phage. This region does not encode any proteins; however, it comprises all the cis-acting sequences required to initiate […]
Hybridization based Screening of Bacteriophage DNA on the filters
Need Digital Health Services for your research? Click here Filters carrying immobilized bacteriophage DNA are labeled with 32P-labelled probes to screen by hybridization in situ. In this procedure, the filters are first submerged in a prehybridization solution (containing blocking solution) to reduce non-specific probe absorption. The filters are then incubated with a denatured radioactive probe […]
Breathomics: Looking Into Breath Analysis for Medical Diagnostics
Want to be part of our Stories? Tell us your story Click here Name: Paul S. Monks Location: Leicester, UK H-index: 49 Twitter followers: 2,236 Hello! Who are you and what are you working on? I’m Paul Monks, and I’m currently the Chief Scientific Advisor to one of the UK government’s departments, the Department of […]
CSP: Apocalypse – Zombies
The Conduct Science Podcast: Apocalypse – Zombies 00:00 – Introduction 02:00 – News 10:30 – What are zombies? 16:05 – Favourite films 26:05 – Our zombie plan 32:50 – The science of zombies 1:02:00 – Ending and outro You can listen to The Conduct Science Podcast by using the player above, searching for “The Conduct […]