Introduction Living organisms require biomolecules for several biological processes like energy storage and regulation of their metabolic cycles. Among all, carbohydrates, nucleic acids, lipids, and
Introduction The membrane’s ability to control the transport of materials across the membrane is one of the essential life processes in organisms. The membrane determines
Site-directed mutagenesis is a technique conceived in the 70s used to mutate specific DNA sequences in vitro. It relies on synthetic short single-stranded DNA fragments, or oligonucleotides,
Introduction Lipids are one of the major macromolecules present in our body, and others include nucleic acids, carbohydrates, and proteins. But unlike the other macro
Enzymes are a group of proteins that catalyze non-spontaneous chemical reactions in any biological system. In an organism, enzymes function as a group of interconnected chemical reactions in
Introduction and History Nucleic acids are an essential class of macromolecules present in all cells of organisms including viruses.[1] They were first discovered by Friedrich
Introduction Allosteric enzymes are a group of biocatalysts that possess common characteristics of an enzyme but do not exhibit a typical Michaelis-Menten kinetic behavior. Instead,
In the past, bacteriophage λ DNA purification was largely achieved through procedures that involved Cesium chloride gradients. However, these methods had a low recovery rate
Introduction Genomic DNA or cDNA clones are analyzed in a simple procedure that begins with the restriction digestion of phage DNA mini preparations. The digested
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