Ion-exchange Chromatography Protocol
Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules
Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules
Introduction High-performance liquid chromatography (HPLC) is a powerful separation technique used for the analysis of ions, proteins, and organic molecules. HPLC is based on the
Introduction and Principle The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong
Introduction: Quantitative Method The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially
Introduction and Principle The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE SDS-PAGE gel system which is a revolutionary high-performance polyacrylamide gel
Introduction: Quantitative Method DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed
Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an
Introduction and Principle Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it
Introduction The Western blot is a biological technique that allows for the specific identification and characterization of proteins. In this technique, the proteins are assorted
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