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Category: Science

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Laboratory Techniques & Protocols

Ion-exchange Chromatography Protocol

Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules

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Laboratory Techniques & Protocols

KJELDAHL Method Protocol

Introduction and Principle The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong

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Protein Quantification Protocols
Laboratory Techniques & Protocols

Protein Quantification Protocols

Introduction: Quantitative Method The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially

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Nucleic Acid Quantification Protocols
Laboratory Techniques & Protocols

Nucleic Acid Quantification Protocols

Introduction: Quantitative Method DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed

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Filtration Chromatography Protocol
Laboratory Techniques & Protocols

Quantitative Phosphate Protocol

Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an

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Laboratory Techniques & Protocols

TRICINE-SDS-PAGE Protocol

Introduction and Principle Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it

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Laboratory Techniques & Protocols

Western blotting Protocol

Introduction The Western blot is a biological technique that allows for the specific identification and characterization of proteins. In this technique, the proteins are assorted

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