High-resolution deep tissue imaging without being highly invasive is difficult to achieve. Therefore, a new imaging method that allows non- or minimally-invasive image acquisition in vivo and in real-time with sub-cellular spatial resolution, and at sub-second sampling rates is needed.

Can be easily integrated into most (Olympus, Nikon, etc) current 2 photon microscopes, and giving an excellent spatiotemporal resolution of <0.3μm

Minimally invasive recordings from deep tissues such as brain regions (e.g. hippocampus) using anesthetized animals or awake animals that are head-fixed

Provides access to any organ or tissue from the surface to deep structures.