Laboratory Techniques & Protocols

Introduction: In the realm of laboratory and animal research, precision and control are paramount. One indispensable tool for achieving these goals is the syringe pump. Whether you’re administering precise doses of medication to a research subject or infusing solutions at a controlled rate, a syringe pump is your trusted ally. However, setting up and using […]

What Is a Colorimeter? The colorimeter is a device used for colorimetry. It measures the absorbance and transmittance of light waves through a liquid sample.[1] The amount of light transmitted or absorbed by the sample gives the concentration of a known solute in a test solution based on Beer-Lambert’s law.[1] Figure: The diagram of a […]

Reference to this article: ConductScience, Fiber Photometry: Definition & Equipment Needed (2022). doi.org/10.55157/CS20221111 Fiber photometry uses spectroscopy to monitor the cellular dynamics in the cell or organ of interest in vivo. It uses photons from the light of a specified wavelength to trigger cells at the region of interest, enabling researchers to monitor the dynamics […]

Centrifuges are one of the most frequently used instruments in research and diagnostic laboratories. They create centrifugal force under the influence of gravity for separating liquid or gas samples into fractions. For example, to separate red blood cells from serum or plasma, or to extract nucleic acids or proteins from biological specimens. How Centrifugation Work? […]

Introduction Microtome is an instrument used in biological labs to cut thin slices or sections of different materials.[1] There are several types available, and the rotary microtome is one among them. The rotary microtome cuts the sections when the tissue blocks are moved in a rotary motion using a handwheel.[1] It’s most commonly used for […]

What is Brain Slice? Electrophysiology refers to studying the electrical properties of biological cells and tissues. Thus, brain or neuronal electrophysiology pertains to a branch of electrophysiology that deals with measuring electric current or voltage changes in one neuronal cell, a brain slice, or the whole brain. The methodology was first introduced by Yamamoto and […]

Tissue slices are viable tissue sections used as in vitro representatives of their originating tissues or organs for metabolic studies, including live-cell imaging, electrophysiological analysis, and pharmacological studies. Theoretically, slices of tissue or organ are immediately used for their intended examinations. However, this is not usually realistic. Tissue slices are prepared once but often examined […]

Introduction Today, we all — biologists or not — have an idea about how the brain functions. The advanced techniques and lab procedures have made the knowledge accessible and unlocked many mysteries related to brain functioning. One such procedure is brain slicing. It’s a process in which parts of the brain are divided using advanced […]

Tissue slices are sections of tissue cut from larger pieces or extracted organs and treated in a medium to gain insights into the working of the originating tissue or organ. Unlike sections for histological studies, tissue slices for metabolic studies are not fixed and preserved. Rather, they are prepared immediately after the excision or after […]