Browse every Science article from the ConductScience research team — experiments, protocols, and reviews from the lab.
We are routinely exposed to radiation. Simply, radiation is the propagation of energy in the form of waves or particles through material in space (Mu et al., 2018). The world is full of background radiation to which we are constantly exposed. Everything we see and hear comes to us in the form of radiation. Radiation […]
Overview of Colorimetry Colorimetry, as the name suggests, means the measurement of colors. In terms of chemical analysis, it is, more specifically, the measurement of the concentration of a particular compound (solute) in a colored solution (solvent). During scientific work, we often need to measure quantities of a particular compound in a mixture or […]
Spectroscopy refers to the interaction between electromagnetic (EM) radiation and matter as a function of its frequency or wavelength. From this, a number of quantitative and qualitative measurements defining aspects like sample concentrations, structure, chemical compositions etc, can be obtained depending on the spectroscopic technique and spectrum of EM radiation used. When EM radiation interacts […]
Need a Spectrophotometer for your Lab? Click here The spectrophotometer is a common optical device that measures the intensity of light relative to color (or wavelength). In the lab, they are used to determine how much light is absorbed by a colored chemical dissolved in the solution. This allows us to calculate the concentration or […]
Introduction Coomassie brilliant blue (CBB) stain is a widely used method for routine visualization of proteins separated on polyacrylamide gels. It is an organic dye that makes complexes with basic amino acids, such as lysine, histidine, tyrosine, and arginine. The stain transfers an overall negative charge to the proteins allowing their separation from the polyacrylamide […]
Introduction Protein separation and identification is critical for proteome analysis and requires high resolution and powerful protein characterization after gel electrophoresis. To identify the proteins in the gel, colorimetric and fluorescence staining techniques are used. The stains include anionic dyes (Coomassie brilliant blue), metal cations (imidazole-zinc), silver stain, fluorescent dyes, and radioactive probes. Specific staining […]
Introduction The reversible Zinc stain is a widely used staining method for the detection of proteins fractionated by polyacrylamide gels or SDS denatured gels. The Zinc reverse staining exploits the ability of biopolymers to bind Zn2+ and that of imidazole to react with unbound Zn2+ to produce insoluble zinc-imidazolate (ZnIm2). This zinc-imidazole complex deposits along […]
Need Incubators for your lab? Click here As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction The pulse-chase analysis is a powerful technique to study the synthesis, processing, and transport of proteins. The pulse-chase analysis is widely used in biochemistry and molecular biology for the examination of the cellular process […]
Need stains for your experiments? Click here Silver staining is a powerful technique for protein identification in gels as silver binds to chemical sidechains of the amino acids, including the carboxyl and sulfhydryl groups. It was introduced in 1972 and later adapted for protein separation from the polyacrylamide gel electrophoresis. The nucleation sites in proteins […]
Need beakers and/or flasks? Click here Acids and Bases play an important role in chemistry. We see them everywhere in our day-to-day lives, from our cleaning agents; soaps and detergents, to baking soda. Acids and bases (alkaline earth metal chemical elements) are 2 categories of corrosive substances. Any compound with a pH value between zero […]
As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction Total internal reflection fluorescence microscopy (TIRFM) is a cutting-edge optical technique that excites the fluorophores in a fragile axial region to visualize the cellular events occurring close to the cell surface. The evanescent field, a near-field wave which decays in intensity […]
Introduction Fluorescence microscopy is a specialized type of light microscopy that relies on the phenomena of fluorescence or phosphorescence. These terms describe materials that absorb light (photons) of one wavelength, then re-emit light of a second, longer wavelength.[1] The difference between fluorescence and phosphorescence is that the latter lasts much longer after the illumination is […]
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