A Chemiluminescence Imaging System operates on the principle of detecting light emitted during a chemical reaction. Chemiluminescence occurs when a chemical reaction produces an excited state intermediate, which then releases energy in the form of light as it returns to a lower energy state. This light emission is captured by the imaging system, typically consisting of a highly sensitive camera (often a cooled CCD or CMOS sensor) and optical components that guide and focus the light onto the detector.
In biological and chemical research, this system is commonly used for detecting and quantifying low levels of specific molecules. For instance, it is widely utilized in Western blotting to detect specific proteins labeled with chemiluminescent substrates. The emitted light corresponds to the presence and concentration of the target molecule, allowing for precise analysis.
The advantage of chemiluminescence imaging lies in its high sensitivity and ability to detect faint signals that might be missed by other methods, making it an invaluable tool for various applications in life sciences, including protein analysis, gene expression studies, and diagnostics.
ConductScience offers the Chemiluminescence Imaging System
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SB-SWE-FP is a newly upgraded low-temperature homogenizer, which can reach a temperature as low as -50ā in the chamber and also equipped with a freezing table.Adopt high-speed reciprocating motion of the vertical shaking system, the frozen sample in the grinding tube collides with the grinding bead, and the resulting grinding shear force and impact force completely break down the tissue. Up to 192 samples can be processed simultaneously in one minute, making it a dedicated device that meets the requirements of rapid processing of multi-sample.
A Chemiluminescence Imaging System operates on the principle of detecting light emitted during a chemical reaction. Chemiluminescence occurs when a chemical reaction produces an excited state intermediate, which then releases energy in the form of light as it returns to a lower energy state. This light emission is captured by the imaging system, typically consisting of a highly sensitive camera (often a cooled CCD or CMOS sensor) and optical components that guide and focus the light onto the detector.
Model | SB-KZ-III | SB-KZ-III-F/FP | SB-KZ-5F-3D | SB-SWE-FP |
|---|---|---|---|---|
Operating mode | The vertical shaking system moves back and forth at high speed. | the vertical shaking system moves back and forth at high speed | Move in a "ā" shaped three-dimensional motion in space | The vertical shaking system moves back and forth at high speed. |
Temperature | Room temperature | -10ā/-40ā | 40ā | -50ā |
Temperature Control precision | N/A | Ā±1ā | Ā±1ā | Ā±1ā |
Standard Spec | 24*2 mL | 24*2 mL | 24*2 mL | 24*2 mL |
Maximum sample volume | 60 pcs | ļ»æ60 pcs | 24 pcs | 92 pcs |
Adapter Specifications | 24x0.5mL. 24x2mL. 32x2mL. 48x2mL. 60x2mL. 12x5mL. 10x10mL. 6x5mL (steel can). 4x30mL (steel can). 2x50mL (steel can) and 8*15mL (steel can) | 24x0.5mL. 24x2mL. 32x2mL. 48x2mL. 60x2mL. 12x5mL. 10x10mL. 6x5mL (steel can). 4x30mL (steel can). 2x50mL (steel can) and 8*15mL (steel can) | 24*2mL. 12*5mL(Optional ) | 24x0.5mL.24x2mL.48x2mL. 32x2mL.60x2mL.96x2mL. 12x5mL.10x10mL.4x30mL(steel can). 6x5mL(steel can).8x15mL(steel can) 2x50mL(steel can).96x2mL(2pcs 96-deep well plate) |
Freezing Table | N/A | N/A | N/A | YES |
Adapter Fixing Method | Nut | Nut | Knob cover with safety locking device | Nut |
Working Time | 0-99min99s | 0-99min99s | 0-99min99s | 0-99min99s |
Dimensions | 365*265*396mm | 370*365*500mm | 370*365*500mm | 650*420*430mm |
Screen Size | 5 inches | 5 inches | 5 inches | 5 inches |
Emergency stop button | YES | YES | YES | YES |
Maximum Feeding Size | No requirements, adjustable according to the adapter. | No requirements, adjustable according to the adapter. | No requirements, adjustable according to the adapter. | No requirements, adjustable according to the adapter. |
Weight | 30.7kg | 45kg | 52kg | 54kg |
Power Parameters | 200-240VAC,50-60 Hz, 200 W | 200-240VAC,50-60 Hz, 400 W | 200-240V AC,50-60 Hz, 400 W | 200-240VAC,50-60 Hz,500 W |
Cooling Method | Low temperature pre-cooling: adapter can reach -80ā or liquid nitrogen pre-cooling | Compressor cooling | Compressor cooling | Compressor cooling |
Transport Screws | Yes (2 at the bottom) | Yes (2 at the bottom) | N/A | Yes (2 at the bottom) |
Advantages | Small size, cost-effective | Compatible with various adapter specifications. Freezing function, lowest temperature can reach -40ā | Higher grinding efficiency, reducing tissue degradation.Freezing function, lowest temperature can reach -40ā. | Lower cooling temperature can reach -50ā. low temperature freezing table, Protects samples and meets low-temperature needs for Western Blot experiments, electrophoresis, and transfer membranes. |
Electromagnetic Safety Lock (all models): Screen click control with automatic power-off unlocking. the cover cannot be opened during operation until the grinding program ends, fully protected.
1.Ā SliderĀ drive,Ā noĀ wearĀ shaft,Ā noiseĀ stabilityĀ andĀ durability;
2. Equipped with low-temperature freezing table. It can meet the needs of sample storage, electrophoresis, membrane transfer and other low-temperature experiments
3. Imported compressor, lowest cooling temperature of -50ā, low temperature grinding throughout the process, reducing degradation of proteins and RNA.
4. Up to 192 samples can be processed simultaneously in 1 minute (24 samples can be processed with standard), efficiently and effectively completing sample grinding with high throughput.
5.Ā MultipleĀ adaptersĀ toĀ choose.
No. | Product Name | Model/Specification | Quantity | Remarks |
|---|---|---|---|---|
1 | Tissue Homogenizer | SB-SWE-FP | 1 | |
2 | Standard Hollow Adapter | 2mL*24 | 1 | Can process 24 samples at once |
3 | Beads Pen | SYM-04Z | 1 | Suitable for 2mL grinding tubes, zirconia beads 4mm |
4 | Beads Pen | SYM-04B | 1 | Suitable for 2mL grinding tubes, steel beads 4mm |
5 | Beads Pen | SYM-03B | 1 | Suitable for 2mL grinding tubes, steel beads 3mm |
6 | 2mL Centrifuge Tube | -- | 1 | Tube wall is thickened, material strength is higher, and there is no RNA enzyme. |
SampleĀ Preparation
The adapter can be used to grind 96 samples simultaneously in a 96-well plate. The adapter includes a base that can hold a 96- well plate, a top cover, and a locking nut.
Take the samples to be ground and place them in the grinding tubes. It is recommended to use no more than 100mg of sample. Add the corresponding extraction solution, ensuring that the total volume of the sample and extraction solution is within half of the overall volume. Add a few appropriately sized grinding beads. To ensure grinding efficiency and quality, for larger volume samples, it is recommended to cut them into smaller pieces using scissors.
Unscrew the locking nut and remove the top cover. Place the grinding tubes into the adapter, then attach the top cover and tighten the locking nut in a clockwise direction. (The placement of the tubes should follow the principles of symmetry and balance.)
OperationĀ Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
AfterĀ theĀ startupĀ interfaceĀ isĀ displayed,Ā waitĀ forĀ aboutĀ 5Ā secondsĀ toĀ enterĀ theĀ mainĀ operatingĀ interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the “Temperature Selection” button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.
EquipmentĀ Maintenance
Perform the following routine maintenanceĀ after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplugĀ the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a)Ā OrdinaryĀ cleaningĀ agent
b)Ā NeutralĀ detergent
c)Ā 70%Ā alcoholĀ contentĀ
Liang, X., Ubhayakar, S., Liederer, B. M., Dean, B., Ran-Ran Qin, A., Shahidi-Latham, S., & Deng, Y. (2011).Ā Evaluation of homogenization techniques for the preparation of mouse tissue samples to support drug discovery. Bioanalysis, 3(17), 1923ā1933.Ā
Smith, K. M., & Xu, Y. (2012).Ā Tissue sample preparation in bioanalytical assays. Bioanalysis, 4(6), 741ā749.Ā doi:10.4155/bio.12.19Ā
Xue, Y. J., Gao, H., Ji, Q. C., Lam, Z., Fang, X., Lin, Z., ā¦ Weng, N. (2012). Bioanalysis of Drug in Tissue: Current Status And Challenges.Ā Bioanalysis,Ā 4(21), 2637ā2653.Ā
| Dimensions | 400mmĆ371mmĆ700mm |
|---|---|
| Industrial Computer | 10.4 inches, 1024Ć768, Windows operating system |
| External Interfaces | 2 USB 3.0 ports |
| Operating Voltage | 100V-240V |
| Product Power | 100W |
| Product Net Weight | 25Kg |
| Model | SB-KZ-III, SB-KZ-III-F/FP, SB-KZ-5F-3D, SB-SWE-FP |
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DISCLAIMER: ConductScience and affiliate products are NOT designed for human consumption, testing, or clinical utilization. They are designed for pre-clinical utilization only. Customers purchasing apparatus for the purposes of scientific research or veterinary care affirm adherence to applicable regulatory bodies for the country in which their research or care is conducted.
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