Browse every Science article from the ConductScience research team — experiments, protocols, and reviews from the lab.
Introduction Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as the “preparation of λ arms.” Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. The following methods are […]
Introduction At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in EMBL3A, the order of restriction site in the left polyclonal site is SalI-BamHI-EcoRI, and that of the right polyclonal site is EcoRI-BamHI-SalI. Digestion of this type of vector with […]
Introduction Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal site using a single restriction enzyme. In this method, ligated DNA is first subjected to phenol: chloroform extraction and standard ethanol precipitation, respectively. It is then treated with restriction […]
Games as Research Tools: Introduction With the increasing dynamics of today’s digital services and technologies, both gaming for entertainment and gaming for utility purposes have become highly popular in everyday life. Given the social and economic impact of games across the globe, it’s no surprise that games and simulations are becoming essential tools in research. […]
Introduction and Historical Perspectives Radiations have been extensively used in medical devices to look into the inner workings of the human body. Wilhelm Roentgen brought a revolution in the medical field by first developing an X-ray machine in 1895 after which, the first radiology department was set up. After the discovery of X-ray, various scientists […]
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Fungi is a group of eukaryotic organisms that include a myriad of single and multicellular organisms that are parasitic or saprophytic in nature. Over 1.5 million species of fungus have been discovered and they are found in a range of habitats. Some characteristic […]
Clustered regularly interspaced short palindromic repeats (CRISPR) technology is a rapid, cheap, and efficient tool to edit genomes at a precise location. CRISPR technology has now been reduced to just two components: Endonuclease and g RNA. Endonuclease makes an incision at the target DNA molecule to be edited, and gRNA is a single-stranded synthetic 20 […]
Introduction and History Recombinant DNA molecules are developed in the laboratory by bringing together genetic materials from multiple organisms to produce new genetic combinations that are not previously found in biological organisms. These new combinations are made to benefit science, medicine, agriculture, and industry (Khan, S et al., 2016). Stanford researcher Paul Berg, 1972, ligated […]
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Histology is a routine lab technique used to evaluate the morphology and structure of cells, tissues, and organs under the microscope. Histological studies of biological samples often lead to the establishment of structure/function relationships, which may have diagnostic and prognostic value in the […]
As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Centrifugation is a laboratory technique routinely used to fractionate a given liquid mixture into single components. The basis of the technique is the application of centripetal forces to a sample by spinning it at high velocity. As a consequence, a centrifugal force originates […]
Need Data Services to share your Data & Information? Click here Data science is a multidisciplinary field; it has more dimensions than there is, as yet, the prospective recognition or measure-up means for the far-reaching explorations. Most of us are still getting to be familiarized with this relatively new phenomenon which emphasizes on: Learning from […]
Back in the times, business intelligence, scientific computing, exploratory statistics, etc., were employed separately to perform the singular task. Then data science emerged, thanks to the number of renowned scholars that incessantly pushed the idea forward.
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