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Horizontal Grid Test
SKU ME-5002
In Stock — ships now$890.00
IncludesStandard care
Motor function assessment apparatus measuring grip strength and coordination in rodents using a vertically positioned wire mesh grid system.
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Louise Corscadden, PhD
Director of Science · ConductScience
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Used in a 2023 paper in Science
Senior author: David R. Liu (2025 Breakthrough Prize in Life Sciences)
For inverted screen testing, juvenile mice were subjected to the horizontal grid test for mice (Maze Engineers) on PND25...
Maze Engineers is part of ConductScience.
Key Specifications
Full details →- Model fit
- Mouse, Rat
- SKU family
- ME-5001/5002
- Sizing
- Mouse: Height 20 cm; Rat: Height 30 cm
- Ordering
- Online checkout and quote request available
- Category
- Behavioral Mazes
- Build notes
- Plexiglass
The Horizontal Grid Test is a motor function assessment apparatus used to evaluate grip strength, motor coordination, and neuromuscular function in laboratory rodents. The apparatus consists of a vertically positioned grid box with transparent plexiglass mounting that allows researchers to observe and record animal behavior during the test procedure.
The test measures an animal's ability to maintain grip and coordinate movement while navigating a wire mesh surface. Subjects are placed on the horizontal grid and their latency to fall, grip strength, and movement patterns are recorded. This paradigm is particularly valuable for assessing motor deficits in disease models and evaluating therapeutic interventions targeting neuromuscular function.
How It Works
The Horizontal Grid Test operates on the principle of gravitational challenge combined with grip strength assessment. Animals are placed on the horizontally oriented wire mesh grid while the apparatus is positioned vertically, requiring subjects to maintain grip against gravitational force to prevent falling.
The transparent plexiglass construction allows for continuous behavioral observation and video recording from multiple angles. The wire mesh openings are sized appropriately for each species (0.8 cm for mice, 1.0 cm for rats) to allow secure grip without entrapment. The black acrylic upper section provides visual contrast while the transparent lower section enables clear observation of limb positioning and grip patterns.
Test parameters typically include latency to fall, number of paw slips, and total time spent on the grid. These measurements provide quantitative assessment of grip strength, motor coordination, and neuromuscular function that can be compared across experimental groups and time points.
Features & Benefits
Species-specific wire mesh openings
Optimized grip dimensions (0.8 cm for mice, 1.0 cm for rats) ensure appropriate challenge level while preventing paw entrapment.
Transparent plexiglass construction
Enables clear behavioral observation and video recording from multiple angles for comprehensive motor assessment.
Vertically positioned grid orientation
Provides gravitational challenge that effectively tests grip strength and motor coordination under controlled conditions.
Dual-section mounting design
Black acrylic upper section provides visual contrast while transparent lower section allows detailed observation of limb positioning.
Standardized dimensions
Consistent grid box sizes (12×12 cm mice, 18×18 cm rats) ensure reproducible testing conditions across studies.
Appropriate height mounting
Species-specific heights (20 cm mice, 30 cm rats) provide optimal testing duration while ensuring animal safety.
Durable plexiglass material
Withstands repeated cleaning cycles and provides long-term stability for longitudinal motor function studies.
Sizes by model
| Measurement | Mouse | Rat |
|---|---|---|
| Height | 20 cm | 30 cm |
- mount
- Mouse: Square grid box 12 cm × 12 cm (L x W)
- Mouse: Wire mesh openings 0.8 cm × 0.8 cm (L x W)
- Mouse: Upper 8 cm of mount: Black Acrylic
- Mouse: Bottom 12 cm of mount: Transparent Acrylic
- Rat: Square grid box 18 cm × 18 cm (L x W)
- Rat: Wire mesh openings 1 cm × 1 cm (L x W)
- Rat: Upper 12 cm of mount: Black Acrylic
- Rat: Bottom 18 cm of mount: Transparent Acrylic
Orientation
- vertically positioned
Apparatus Type
- grid box
Behavioral Construct
- Motor Coordination
- Grip Strength
- Neuromuscular Function
Automation Level
- manual
Material
- Plexiglass
Species
- Mouse
- Rat
Research Domain
- Behavioral Pharmacology
- Motor Function
- Neurodegeneration
- Neuroscience
- Pain Research
- Toxicology
Compatible Tracking Software
- ConductVision
Shipping weight
- 21.0 lb
Shipping box
- L: 43.2 cm
- W: 38.0 cm
- H: 27.9 cm
| Feature | This Product | Typical Alternative | Advantage |
|---|---|---|---|
| Grid Orientation | Horizontally positioned grid with vertical apparatus mounting | Inverted or angled grid configurations | Natural orientation reduces test anxiety and provides physiologically relevant motor challenge. |
| Observation Access | Transparent plexiglass construction with dual-section design | Opaque or limited visibility apparatus designs | Enables comprehensive behavioral observation and video analysis from multiple angles. |
| Species Accommodation | Dedicated mouse and rat configurations with species-specific dimensions | Single-size apparatus requiring adaptations | Optimized grip challenge and safety parameters for each species without modifications. |
| Wire Mesh Specifications | Precisely sized openings (0.8 cm mice, 1.0 cm rats) | Standard mesh sizes not optimized for grip testing | Prevents paw entrapment while ensuring appropriate grip challenge for reliable motor assessment. |
| Height Configuration | Species-appropriate mounting heights (20 cm mice, 30 cm rats) | Fixed height systems requiring platform adjustments | Eliminates need for additional equipment while maintaining consistent testing conditions. |
This horizontal grid apparatus provides species-optimized motor testing with transparent observation access and natural grip orientation. The dual-section plexiglass design enables comprehensive behavioral analysis while maintaining appropriate challenge levels for reliable motor function assessment.
| Model | Size | SKU | Listed price | Status | Shipping box |
|---|---|---|---|---|---|
| Mouse | Height 20 cm | ME-5001 | $790.00 | Available | 43.2 x 38.0 x 27.9 cm |
| Rat | Height 30 cm | ME-5002 | $890.00 | Available | 43.2 x 38.0 x 27.9 cm |
Neuroscience
Assessing motor deficits in Parkinson's disease models by measuring grip strength and coordination during grid navigation tasks.
Behavioral Pharmacology
Evaluating motor side effects of pharmaceutical compounds by comparing pre- and post-treatment performance on grip strength tests.
Motor Function
Quantifying neuromuscular function changes in aging studies by measuring latency to fall and grip maintenance duration.
Neurodegeneration
Monitoring disease progression in ALS or muscular dystrophy models through longitudinal grip strength and motor coordination assessments.
Pain Research
Assessing mechanical allodynia and motor function impairment in neuropathic pain models using grip strength measurements.
Toxicology
Screening for neurotoxic effects of test compounds by evaluating motor coordination and grip strength deficits.
Practical Tips
Calibration
Verify grid positioning is perfectly vertical using a level before each testing session.
Why: Even slight angular deviations can affect gravitational challenge and introduce measurement bias.
Maintenance
Inspect wire mesh weekly for damage or deformation that could affect grip uniformity.
Why: Damaged mesh creates inconsistent grip challenges and potential safety hazards for test subjects.
Best Practices
Allow animals to acclimate to the apparatus for 30-60 seconds before starting timed trials.
Why: Reduces novelty stress and provides more reliable baseline motor function measurements.
Data Quality
Record environmental temperature and humidity as these factors can affect grip performance.
Why: Temperature changes affect paw pad moisture and grip adhesion, potentially confounding motor assessments.
Troubleshooting
If animals consistently fall immediately, check for residual cleaning solution or moisture on the grid surface.
Why: Chemical residues or dampness reduce grip friction and produce artificially poor performance scores.
Safety
Position soft padding beneath the apparatus to prevent injury from falls during testing.
Why: Protects animals from impact injury while maintaining the psychological motivation to grip the mesh.
Best Practices
Conduct testing at consistent times of day to control for circadian effects on motor performance.
Why: Motor coordination and grip strength can vary significantly across the light-dark cycle in rodents.
Data Quality
Use high-speed video recording to capture subtle paw slip events that may be missed by observers.
Why: Detailed movement analysis provides more sensitive detection of motor deficits than timing alone.
Setup Guide
Unpack and Inspect
Remove apparatus from packaging and inspect grid box, plexiglass mount, and wire mesh for damage or defects.
Position Apparatus
Mount the grid box vertically using the provided mounting system, ensuring stable positioning and proper height adjustment.
Clean Grid Surface
Clean wire mesh thoroughly with 70% ethanol and allow to dry completely before first use.
Setup Recording Equipment
Position video camera or timing equipment to capture animal behavior through the transparent plexiglass sections.
Environmental Preparation
Ensure testing room has appropriate lighting and minimal noise distractions for consistent behavioral assessment.
Protocol Validation
Conduct practice trials with control animals to establish baseline measurements and verify timing accuracy.
Data Collection Setup
Prepare data sheets or electronic recording systems for capturing latency times, grip patterns, and behavioral observations.
What’s in the Box
- Grid box apparatus with wire mesh (species-specific)
- Vertical mounting system with height adjustment
- Plexiglass enclosure (black and transparent sections)
- Assembly hardware and mounting brackets (typical)
- User manual and protocol guide (typical)
Warranty
ConductScience provides a standard one-year manufacturer warranty covering defects in materials and workmanship, with technical support for setup and protocol development.
Compliance
ARRIVE GuidelinesSupports standardized reporting of motor function experiments in animal research through consistent apparatus design and measurement protocols.
Guide for the Care and Use of Laboratory AnimalsApparatus design considerations align with humane animal testing principles for behavioral and motor function assessment.
References
Background reading relevant to this product:
1.Sung Tae Kim et al. (2009) “Vertical grid test and modified horizontal grid test are sensitive methods for evaluating motor dysfunctions in the MPTP mouse model of Parkinson's disease” Brain Research doi:10.1016/j.brainres.2009.09.103
Q
What is the optimal trial duration for grid testing protocols?
A
Trial duration typically ranges from 60-180 seconds depending on study objectives. Shorter durations assess initial grip strength while longer trials evaluate endurance and fatigue. Consult product datasheet for species-specific recommendations.
Q
How should the apparatus be cleaned between animal trials?
A
Clean wire mesh and plexiglass surfaces with 70% ethanol between subjects to prevent scent transfer and maintain hygiene. Allow complete drying before next trial to prevent slipping artifacts.
Q
What behavioral parameters should be recorded during testing?
A
Key measurements include latency to first fall, total time maintained on grid, number of paw slips, and grip pattern observations. Video recording enables post-hoc analysis of limb coordination and movement strategies.
Q
Can this apparatus be used for longitudinal studies?
A
Yes, the durable plexiglass construction supports repeated testing over weeks or months. Maintain consistent environmental conditions and testing protocols to ensure reliable longitudinal comparisons.
Q
How does this compare to inverted screen tests?
A
The horizontal grid provides gravitational challenge in a natural orientation while inverted screens test against gravity in an unnatural position. Grid testing may be less stressful and more physiologically relevant for motor assessment.
Q
What statistical considerations apply to grid test data?
A
Latency data often requires non-parametric analysis due to ceiling effects. Consider survival analysis methods for time-to-fall data and repeated measures designs for longitudinal studies.
Q
Are there specific handling protocols for different disease models?
A
Disease models with severe motor deficits may require modified protocols including shorter maximum trial times and careful monitoring for animal welfare. Establish humane endpoints before testing begins.
Have a question about this product?
Track rodents with ConductVision
ConductVision is our video-tracking software. Record your sessions on video, and it scores standard rodent tests, such as those listed below.
Examples of tests it scores
To check whether ConductVision scores your protocol in this apparatus, ask in your quote request.
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Use this apparatus with
The complete Horizontal Grid Test workflow
Track behavior
No exact ConductVision horizontal-grid page is currently published. Hang latency and foot faults are normally captured by a stopwatch and frame-by-frame video rather than overhead tracking; keep automated grip detection as a roadmap gap.
Supporting page not yet builtRun protocol
No exact ConductMaze horizontal-grid protocol is currently published. Grid spacing, inversion timing, the holding-impulse definition, and re-grip rules belong here once the protocol page ships; keep this as a roadmap gap.
Supporting page not yet builtAnalyze output
No published grid-specific analyzer is currently available. Latency to fall, foot faults, and holding impulse are normally summarized in a spreadsheet against body weight; keep a dedicated grid scorer as a roadmap gap.
Supporting page not yet builtConfiguration considerations
Common Horizontal Grid Test setup decisions
Use these notes to scope species, cohort, tracking, and automation needs. Only verified product or support routes are linked from this section.
This productInvertible grid
Horizontal Grid Apparatus
Wire-mesh grid in an invertible frame with padded landing surface below
Standard configuration for grip endurance and limb placement, scoring latency to fall and foot faults as the animal holds onto a grid that is inverted above a padded surface.
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Request QuoteBuyableMouse or rat
Species-Scaled Grid
Wire diameter and grid spacing scaled for mouse or rat paw size
Wire diameter and grid spacing change grip mechanics and hold times, so the grid geometry should match the paw size and body weight of the species being tested.
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View options ->SpecialtyFoot-fault scoring
Horizontal-Walk Grid
Horizontal grid walkway with side-view camera for foot-fault counting
Best when the question is limb placement rather than grip endurance, scoring foot faults as the animal walks across a horizontal grid rather than hanging from an inverted one.
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Request automation help§ 1
Introduction
The Horizontal Grid Test measures neuromuscular grip endurance and limb placement by recording how long a rodent holds onto a wire grid and how often its paws slip through the openings. Tillerson and colleagues used grid-based measures to detect sensorimotor impairments that track neurochemical deficits in mouse models. 1
In the inverted configuration the grid is rotated above a padded surface and the apparatus records latency to fall and maximal hang time, while a horizontal-walk configuration scores foot faults as paws slip through the grid. Together these give a combined index of grip strength, endurance, and limb placement that is sensitive to muscle and motor-system deficits. 1
Body weight, motivation, grid spacing and wire diameter, fatigue across trials, and repeated-trial learning all change hold times and foot faults independent of true neuromuscular function. A defensible protocol fixes the grid geometry and inversion timing, reports body weight, standardizes inter-trial rest, and separates the first trial from later, learning-influenced trials. 1
§ 2
Methods
2.1 Procedure
Inverted-grid hang testing with foot-fault scoring, holding-impulse calculation, and standardized inter-trial rest.
Pre-test setup
- 1.Acclimation and habituation: Habituate animals to the room and to brief handling so the first measured trial reflects neuromuscular function rather than novelty or handling stress.
- 2.Apparatus calibration: Verify grid spacing, wire diameter, inversion height, and the padded landing surface, and confirm the timer and side-view camera capture the moment of fall and individual foot faults.
- 3.Set the protocol: Fix the number of trials, the maximum hang duration, the inter-trial rest interval, and whether the test uses the inverted-hang or horizontal-walk configuration before any data are collected.
- 4.Record body weight: Weigh each animal before testing, because body weight is a major non-neuromuscular driver of hang time and is needed to compute holding impulse.
Trial sequence
- 1.Place on the grid: Allow the animal to grip the grid with all four paws, then invert or position the grid according to the protocol and start the timer.
- 2.Record latency to fall: Stop the timer when the animal releases and drops onto the padded surface, capping at the pre-defined maximum hang duration.2
- 3.Score foot faults: Count foot faults where a paw slips through the grid openings, scored from side-view video in the horizontal-walk configuration.4
- 4.Compute holding impulse: Multiply hang time by body weight to express a holding impulse that accounts for the load the animal supported.
- 5.Rest and repeat: Allow the planned inter-trial rest to limit fatigue, then clean the grid between subjects to remove odor and urine cues before the next trial.
Critical methodological constraints
- Body weight. Heavier animals fall sooner from an inverted grid independent of neuromuscular function. Report body weight and express results as holding impulse where possible.4
- Grid geometry. Wire diameter and grid spacing set grip difficulty. Geometry must be held constant across groups so hang times and foot faults are comparable.2
- Fatigue across trials. Repeated hangs with short rest reduce hold times through fatigue rather than a stable deficit. Standardize the inter-trial rest interval.
- Repeated-trial learning. Animals can improve grip strategy across trials, so the first trial and later trials measure partly different things. Separate them when interpreting group differences.3
2.2 Measurement & Analysis
Core horizontal-grid endpoints for grip endurance, limb placement, and quality control.
Latency To Fall
Grip endurance (inverted grid)
Time the animal holds onto the inverted grid before releasing, the primary grip-endurance readout.2
Foot Faults
Limb placement
Count of paw slips through the grid openings in the horizontal-walk configuration, indexing limb placement accuracy.4
Holding Impulse
Strength-time
Hang time multiplied by body weight, a strength-time product that adjusts grip endurance for the load supported.
Maximal Hang Time
Endurance ceiling
The longest single hang across trials, capped at the protocol maximum, indexing the endurance ceiling.2
Re-grip Attempts
Quality-control flag
Number of times the animal re-grips or shifts position during a hang; high counts flag an unstable or invalid trial.
+ Additional metrics: body weight, trial number, inter-trial interval, grid spacing, configuration used, and per-trial video notes.
2.3 hold fraction (analysis)
A compact fraction of the testing window the animal spent holding onto the grid.
2.4 sample-size planning
Estimate the N per group needed to detect a literature-anchored motor effect at the endpoint you plan to report. Override the defaults with your own pilot numbers.
§ 3
Results
PubMed publication counts, sample apparatus output, and recent papers from a dated PubMed snapshot.
3.1 Publication trends
PubMed volume and co-occurring behavioral methods for horizontal-grid neuromuscular studies.
3.2 Sample apparatus output
Representative output from an inverted-grid hang session capped at a 180 s maximum hang duration.
3.3 Recent findings (PubMed)
- Sep 2026PMID: 42736231
Neuroprotective effects of Moringa oleifera leaf extract via potential NF-κB modulation in cuprizone-induced demyelination in rats: Integrated in vivo and in silico evidence.
Essawy AE, Fahmy RI, Gomaa RA, et al.. Tissue Cell. 2026 Sep 8.
Myelin sheath impairment is linked to several neurological disorders; however, effectively counteracting its pathological consequences remains a formidable challenge.
- Sep 2026PMID: 42711364
Neuroprotective effects of empagliflozin and metformin combined therapy on rotenone induced Parkinsonism in Wistar rats via PI3K and PPARγ modulation.
Mona MM, Borg HM, Ahmed AA, et al.. Sci Rep. 2026 Sep 8.
Parkinsonism is a progressive neurodegenerative disorder characterized by dopaminergic neuronal loss and α-synuclein aggregation within the substantia nigra pars compacta (SNc).
- Sep 2026PMID: 42128255
Anti-inflammatory and anti-oxidative effects of vanadium on motor and cerebellar cortices of juvenile hydrocephalic mice.
Olopade FE, Femi-Akinlosotu OM, Ugwuorah JC, et al.. Exp Neurol. 2026 Sep.
Hydrocephalus presents a significant clinical neurology challenge, manifesting complications such as neuronal degeneration, cognitive impairments and motor deficits. Ventricular shunting is the primary recourse for treatment, but it is fraught with complications such as infection and obstruction.
- Aug 2026PMID: 41225278
Widespread neuronal hemokinin-1 expression in motor regions and its protective role in age-related impairment.
Simon DV, Rozmer K, Kepe E, et al.. Geroscience. 2026 Aug.
Hemokinin-1 (HK-1) regulates several processes in the central and peripheral nervous systems, including musculoskeletal functions; however, its involvement in age-related motor coordination and muscle strength remains unknown.
- Jul 2026PMID: 42530254
Peripheral Administration of isoD7-Aβ Exacerbates Motor Deficits and Tau Pathology in P301S Mice.
Stepenko Y, Shcheblykina O, Zhunusov N, et al.. Front Biosci (Landmark Ed). 2026 Jul 24.
Alzheimer's disease (AD) is a progressive neurodegenerative disorder leading to dementia, disability, and premature death. Beta-amyloid (Aβ) and tau pathology are considered key components of its pathogenesis; however, the role of peripheral amyloid load in modulating pre-existing tau pathology remains understudied.
- Jul 2026PMID: 42384247
Neuroprotective effects of 2-(2-nitrophenyl)-1,3-thiazolidine-4-carboxylic acid in a rotenone-induced Parkinson's disease rat model: Modulation of oxidative stress, behavioral dysfunction, and iron accumulation.
Kousar S, Perveen S, Haider I, et al.. Metab Brain Dis. 2026 Jul 1.
Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by dopaminergic neuronal loss, oxidative stress, and abnormal iron accumulation in the substantia nigra. Targeting redox imbalance and metal dysregulation represents a promising therapeutic strategy.
§ 4
Discussion
Limitations of the paradigm, methodological caveats, and current directions.
4.1 Common confounds
Variables that can shift Horizontal Grid Test results apart from the effect under study.
Body weight
Heavier animals fall sooner from an inverted grid independent of neuromuscular function. Report weight and express results as holding impulse where possible.
Motivation
A poorly motivated or stressed animal may release early without a true strength deficit, so handling and habituation must be standardized.
Grid spacing and wire diameter
Grid geometry sets grip difficulty. Wire diameter and spacing must be held constant across groups for hang times and foot faults to be comparable.
Fatigue across trials
Repeated hangs with short rest reduce hold times through fatigue rather than a stable deficit. Standardize the inter-trial rest interval.
Repeated-trial learning
Animals can improve grip strategy across trials, so the first trial and later trials measure partly different things. Separate them when interpreting differences.
Preview exported markdown
## Horizontal Grid Test — methods controls Confounds controlled in this protocol: - **Body weight.** Heavier animals fall sooner from an inverted grid independent of neuromuscular function. Report weight and express results as holding impulse where possible. - **Motivation.** A poorly motivated or stressed animal may release early without a true strength deficit, so handling and habituation must be standardized. - **Grid spacing and wire diameter.** Grid geometry sets grip difficulty. Wire diameter and spacing must be held constant across groups for hang times and foot faults to be comparable. - **Fatigue across trials.** Repeated hangs with short rest reduce hold times through fatigue rather than a stable deficit. Standardize the inter-trial rest interval. - **Repeated-trial learning.** Animals can improve grip strategy across trials, so the first trial and later trials measure partly different things. Separate them when interpreting differences.
4.2 Construct validity caveats
The horizontal grid test is strongest when grid geometry, inversion timing, and inter-trial rest are fixed, body weight is reported, and the first trial is separated from later, learning-influenced trials. A single hang time is a screening signal; confirm neuromuscular deficits with holding impulse and an independent strength or coordination assay such as grip strength or the rotarod. 1
4.3 Special considerations
Inverted hang or horizontal walk?
Use the inverted-hang configuration for grip endurance and the horizontal-walk configuration for limb placement and foot faults. The two index different functions and should be reported as distinct endpoints.
Should I report body weight?
Yes. Body weight is one of the largest non-neuromuscular drivers of hang time and is required to compute holding impulse, which adjusts grip endurance for the load the animal supported.
How do I limit fatigue effects?
Standardize the inter-trial rest interval and cap the number of trials. Repeated hangs with short rest reduce hold times through fatigue rather than a stable deficit.
4.4 Current directions
Quarterly editorial review of emerging Horizontal Grid Test methodology. Q2 2026
Methods
Holding-impulse standardization
Expressing grip endurance as hang time multiplied by body weight improves comparability across cohorts that differ in mass.
Emerging
Video-based foot-fault scoring
High-frame-rate side-view video improves foot-fault counting consistency in the horizontal-walk configuration and reduces observer burden.
Methods
Standardized grid geometry
Reporting wire diameter and grid spacing is increasingly expected because grid geometry sets grip difficulty and hang times.
Emerging
Multi-assay motor batteries
The horizontal grid is paired with grip strength, rotarod, and gait analysis to separate neuromuscular strength from coordination and limb placement.
§ 5
References
5 selected methods and validation references for Horizontal Grid Test.
- Tillerson JL, Caudle WM, Reveron ME, Miller GW. Detection of behavioral impairments correlated to neurochemical deficits in mice treated with moderate doses of MPTP. Exp Neurol. 2002;178(1):80-90. doi:10.1006/exnr.2002.8021
- Deacon RM. Measuring the strength of mice. J Vis Exp. 2013;(76):2610. doi:10.3791/2610
- Aartsma-Rus A, van Putten M. Assessing functional performance in the mdx mouse model. J Vis Exp. 2014;(85):51303. doi:10.3791/51303
- Carlson CG, Rutter J, Bledsoe C, et al. A simple protocol for assessing inter-trial and inter-examiner reliability for two noninvasive measures of limb muscle strength. J Neurosci Methods. 2010;186(2):226-230. doi:10.1016/j.jneumeth.2009.11.006
- Brooks SP, Dunnett SB. Tests to assess motor phenotype in mice: a user's guide. Nat Rev Neurosci. 2009;10(7):519-529. doi:10.1038/nrn2652
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