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Gait Analysis

Tracking Locomotion & Motor Deficits

Explore locomotion and motor disorders in rodents like never before with automated Gait Analysis. Reveal key gait parameters—like paw state, base of support, and phase difference—using AI-driven tracking.

Key Results Metrics

What is Gait Analysis?

Gait analysis measures how subjects walk and tracks potential abnormalities. It includes parameters such as

  • Paw details: Stance or swing state, base of support
  • Stride details: stride length, duration, duty cycle
  • Gait coordination: Phase difference in degrees

Click our parameters below for more details.

Stride Length

Stance vs. Swing Phase

Interlimb Coordination

Gait Symmetry

Base of Support

Paw Overlap

AI-Based Methods

ConductVision uses our advanced AI techniques with the Hidden Markov Model (HMM), smooth temporal segmentation of paw activities is achieved to classify paw movement metrics.

This is done through automatic tracking body key points (nose, fore- and hindpaws, and body) to calculate paw movement and spatial distance. No manual labelling, automatic recognition of each key body point.

Some of the Parameters Measured

Compatible Mazes

Definitions

Swing versus Stance: The stance and swing phases are defined periods of the Gait cycle that take place when the mouse is walking or running.

Parameter Stance Phase Swing Phase
Definition
The period during which a paw is in contact with the ground and supporting body weight. Includes braking and propulsion durations
The period when the paw is lifted off the ground and moves forward to initiate the next step.
Function
Provides stability, weight-bearing, and propulsion
Responsible for advancing the limb in preparation for the next stance.
Duration
Typically longer than swing phase during normal walking. Approximately 70% of the gait cycle
horter than stance phase in healthy gait. Approximately 30% of the gait cycle
Graph showing changes in paw contact area over time during gait analysis, illustrating stride, stance, swing, braking, and propulsion phases with overlapping bell curves.
Diagram illustrating gait phases in trisomic mice, including braking time, propulsion time, and swing time, mapped onto stance and stride durations.

↑ Stance Phase → Often indicates instability, weakness, or cautious movement.

↓ Stance Phase → Often due to pain avoidance or reduced weight-bearing.

↑ Swing Phase → May signal reluctance to place the paw, or dragging.

↓ Swing Phase → Indicates rigidity, bradykinesia, or hyperkinetic drive

Duty Cycle: The ratio of the Stance Duration (the time the paw is on the ground) to the total Stride Duration (Stance Duration + Swing Duration)

Typically expressed as a percentage.

 

↑ Duty Cycle Often indicates a strategy to maximize stability or increase weight-bearing time, typically due to weakness, general fatigue, or a compensation for instability (ataxia/loss of balance).

↓ Duty Cycle Often indicates a need to quickly remove the paw from the ground, typically due to pain avoidance (antalgic gait) or a compensation for rigidity/bradykinesia to maintain forward momentum, but is also a natural result of increased speed and gait efficiency.

 

Base of Support: The distance between the two paws on the same side (fore- or hind-) or the two hind paws or two front paws

unnamed (6)

↑ Base of Support (BoS) Often indicates a need for increased stability, typically due to loss of balance, general weakness, or ataxia (lack of voluntary coordination).

↓ Base of Support (BoS)→  May signal a shift toward cautious/narrow-based movement in some forms of rigidity, or is often a goal for increased gait efficiency (closer to a straight line) in healthy, confident movement.

Phase Difference: The relative timing between two limbs (often left vs. right or fore vs. hind). It tells you how synchronized two paws are within the stride cycle.

Our software defines the Phase difference in degrees:

Phase Difference (in ∘) Synchronization Pattern Interpretation
Zero
Synchronization (In-Phase)
Both paws are moving together; Paw B begins its stance phase at the exact same moment (or the end of a cycle) as Paw A’s stride begins. This is common for bilateral forelimbs or hindlimbs in abnormal/pathological gaits (e.g., “bunny hopping”) but is pathological if seen in alternating gaits.
180
Alternation (Anti-Phase)
Paw B begins its stance phase at the midpoint of Paw A’s stride cycle. This represents ideal coordination for alternating diagonal (e.g., right-fore to left-hind) or alternating lateral (e.g., right-fore to left-fore) movements in a typical healthy rodent gait.
90/270
Compromised Alternation
Paw B begins its stance phase when Paw A is one-quarter ($1/4$) of the way through its stride cycle. This indicates a significant mistiming or asymmetry often seen in uncoordinated or pathological gaits.
Close to 0
Clustering/Dragging
Paws are moving too close together, potentially indicating weakness or dragging.
High Variability/Spread
Ataxia/Lack of Control
The differences are highly inconsistent across strides. This suggests ataxia (loss of muscle control), or severe neurological deficits affecting rhythm and control

Neurological Disorders

Parkinson’s Disease (PD)

Shuffling steps, reduced stride, asymmetry

Stroke

Unilateral limb weakness, asymmetry

Multiple Sclerosis (MS)

Spasticity, Ataxia

Amyotrophic Lateral Sclerosis (ALS)

Progressive hindlimb weakness

Spinal Cord Injury (SCI)

Limb paralysis, recovery tracking

Peripheral Neuropathy

Foot dragging, altered paw placement

Cerebellar Ataxia

Wide stance, uncoordinated movements

Applications

Musculoskeletal Disorders

Osteoarthritis

Limping, reduced weight-bearing

Rheumatoid Arthritis

Joint stiffness

Muscular Dystrophy

Weak muscles, short stride

Pain Models

Inflammatory Pain

Altered weight-bearing, paw guarding

Neuropathic Pain

Limping, gait cycle changes

Genetic & Developmental Disorders

Autism Spectrum Disorders (ASD)

Motor coordination deficits

Huntington’s Disease

Hyper/hypokinetic movements

Metabolic Disorders

Diabetes Mellitus

Diabetic neuropathy impacts gait

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