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Zebrafish Y Maze Flow Modification

See more by: MazeEngineers

$1,090.00

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Ā / Availability: In Stock / Delivery Info ā“˜

Description

Meet the producer

Producer: MazeEngineers

MazeEngineers offers custom-built behavioral mazes at no extra cost—designed to fit your exact research needs. Eliminate reproducibility issues from poor sizing or lingering scent cues with precision-engineered, modular, and smart mazes that adapt in real time to animal behavior. Publish new protocols, run adaptive experiments, and push the boundaries of behavioral science.

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Description

Features

25 cm long; 8 cm wide and 15 cm high

Apparatus and Equipment

The Y-shaped maze consists of a transparent acrylic tank with dimensions designed as follows: the starting arm measures 18 cm in length and 10 cm in width, while each of the goal arms extends to 30 cm in length and 5 cm in width. The entire structure stands at a height of 20 cm. All three arms are fitted with 1 cm diameter polyethylene tubing at their intake (goal arms) and outflow (start arm) openings. To regulate and measure fluid flow rates, a flow meter is positioned at the intake opening. The inflow of fluid is achieved through the use of elevated water cylinders (utilizing gravity), which are connected to the tubing system.

Training Protocol

The experimental subjects should be housed in tanks that are consistently maintained at a stable temperature and pH level. These environmental conditions should be rigorously upheld throughout the experimental sessions to ensure the well-being and minimize stress for the fish. Adequate aeration of the water and maintaining its quality at optimal levels are equally crucial.

The Y-Maze experimental tank should be filled with water up to a depth of 7 cm to ensure minimal stress on the fish during trials. Position the Y-Maze tank inside a 50 cm tall opaque plastic enclosure placed on Styrofoam sheets to eliminate external visual cues and vibrations. Illuminate the tank from above for clear observation. For automated tracking and video recording of fish behaviors and movements, utilize specialized software like Noldus Ethovision XT installed on a ceiling-mounted system above the tank.

Between each experimental subject, ensure thorough emptying and rinsing of the tank to maintain cleanliness and eliminate residual influences.

Data Analysis

The following data can be observed in the flow-through Y-Maze tasks,

Strengths and Limitations

Summary

  • The flow-through Y-Maze is a modification of the Zebrafish Y-Maze.
  • The ā€˜Y’ shaped design uses a more natural turn angle.
  • Flow meter allows controlling and measuring the flow rate of fluids.
  • The flow-through Y-Maze can be effectively used to assess preference and avoidance behaviors in fishes.
  • During testing, it is important to eliminate any disturbances that could potentially affect the subject’s performance.
  • Handling and habituation process can also impact maze performance.

References

Grella, S. L, Kapur, N., & Gerlai, R. (2010). A Y-maze Choice Task Fails to Detect Alcohol Avoidance or Alcohol Preference in Zebrafish. International Journal of Comparative Psychology, 23(1)

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